Measuring Sleep in Drosophila

Miyazaki, Takeshi; Williams, Julie; Toda, Hirofumi

doi:10.1007/978-1-0716-2321-3_4

Cited by 2 publications

(1 citation statement)

References 65 publications

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“…Flies that were already asleep from before these time points were excluded from the analysis of latency values (in minutes). Data were processed using custom software, Insomniac 3 24 . For each locomotor assay, 8 flies (for MB5 monitors) to 16 flies (for DAM5H or DAM2 monitors) per group were loaded into monitors for each of 2-4 experimental replicates.…”

Section: Behavioral Assaysmentioning

confidence: 99%

The NFκBDifis required for behavioral and molecular correlates of sleep homeostasis inDrosophila

O’Hara,

Saul,

Handa

et al. 2023

Preprint

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The nuclear factor binding the κ light chain in B-cells (NFκB) is involved in a wide range of cellular processes including development, growth, innate immunity, and sleep. However, efforts have been limited toward understanding how specific NFκB transcription factors function in sleep.Drosophilafruit flies carry three genes encoding NFκB transcription factors,Dorsal,Dorsal Immunity Factor(Dif), andRelish. We previously found that loss of theRelishgene from fat body suppressed daily nighttime sleep, and abolished infection-induced sleep. Here we show thatDifregulates daily sleep and recovery sleep following prolonged wakefulness. Mutants ofDifshowed reduced daily sleep and suppressed recovery in response to sleep deprivation. Pan-neuronal knockdown ofDifstrongly suppressed daily sleep, indicating that in contrast toRelish,Diffunctions from the central nervous system to regulate sleep. Based on the distribution of aDif-associated GAL4 driver, we hypothesized that its effects on sleep were mediated by the pars intercerebralis (PI). While RNAi knock-down ofDifin the PI reduced daily sleep, it had no effect on the recovery response to sleep deprivation. However, recovery sleep was suppressed when RNAi knock-down ofDifwas distributed across a wider range of neurons. Induction of thenemuri(nur) antimicrobial peptide by sleep deprivation was suppressed inDifmutants and pan-neuronal over-expression ofnuralso suppressed theDifmutant phenotype. Together, these findings indicate thatDiffunctions from brain to targetnemuriand to promote sleep.

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Section: Behavioral Assaysmentioning

confidence: 99%

The NFκBDifis required for behavioral and molecular correlates of sleep homeostasis inDrosophila

O’Hara,

Saul,

Handa

et al. 2023

Preprint

Self Cite

View full text Add to dashboard Cite

show abstract

The NFκB Dif is required for behavioral and molecular correlates of sleep homeostasis in Drosophila

O’Hara,

Saul,

Handa

et al. 2024

Sleep

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The nuclear factor binding the κ light chain in B-cells (NFκB) is involved in a wide range of cellular processes including development, growth, innate immunity, and sleep. However, genetic studies of the role of specific NFκB transcription factors in sleep have been limited. Drosophila fruit flies carry three genes encoding NFκB transcription factors, Dorsal, Dorsal Immunity Factor (Dif), and Relish. We previously found that loss of the Relish gene from fat body suppressed daily nighttime sleep, and abolished infection-induced sleep. Here we show that Dif regulates daily sleep and recovery sleep following prolonged wakefulness. Mutants of Dif showed reduced daily sleep and suppressed recovery in response to sleep deprivation. Pan-neuronal knockdown of Dif strongly suppressed daily sleep, indicating that in contrast to Relish, Dif functions from the central nervous system to regulate sleep. Based on the unique expression pattern of a Dif- GAL4 driver, we hypothesized that its effects on sleep were mediated by the pars intercerebralis (PI). While RNAi knock-down of Dif in the PI reduced daily sleep, it had no effect on the recovery response to sleep deprivation. However, recovery sleep was suppressed when RNAi knock-down of Dif was distributed across a wider range of neurons. Induction of the nemuri (nur) antimicrobial peptide by sleep deprivation was reduced in Dif mutants and pan-neuronal over-expression of nur also suppressed the Dif mutant phenotype by significantly increasing sleep and reducing nighttime arousability. Together, these findings indicate that Dif functions from brain to target nemuri and to promote deep sleep.

show abstract

Measuring Sleep in Drosophila

Cited by 2 publications

References 65 publications

The NFκBDifis required for behavioral and molecular correlates of sleep homeostasis inDrosophila

The NFκBDifis required for behavioral and molecular correlates of sleep homeostasis inDrosophila

The NFκB Dif is required for behavioral and molecular correlates of sleep homeostasis in Drosophila

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