Background: Concerning demyelination's process in multiple sclerosis (MS), speech changes often occur earlier than other symptoms; thus early diagnosis of these changes is necessary. According to recent studies, phonation subsystem appears with the most symptoms compared with other subsystems, especially in the initial stages. In this study, the researchers aimed at comparing these symptoms in MS patients with healthy people using dysphonia severity index (DSI) and its parameters. Methods: This study was conducted on 40 MS patients and 20 healthy individuals in Ahvaz city. The subjects were asked to phonate vowel /a/ for calculating maximum phonation time (MPT), maximum F0, jitter, minimum intensity, and DSI score with the Praat software. For statistical analysis of data, Kolmogorov-Smirnov and independent t-test were used.