Measuring the estrus cycle and its effect on superovulation in mice

Zhu, Na; HongXiang, Jia; Liu, Xiaokun; Zhao, Xiaoe; Wei, Qiang; Ma, Baohua

doi:10.3724/sp.j.1141.2012.03276

Cited by 2 publications

(2 citation statements)

References 11 publications

(5 reference statements)

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“…the prooestrus, oestrus, metoestrus and dioestrus stages. The results were consistent with previous reports (Fu et al 2005;Zhu et al 2012;Gravina et al 2014). …”

Section: Discussionsupporting

confidence: 83%

“…The synchronisation effect in mice decreased with successive generations within 12 generations (Schuler 1977). However, there is little information available about the comparative effects of different exogenous hormones on fertility and uterine development in mice (Zhu et al 2012). The oestrus synchronization of mice was successful in this study.…”

Section: Discussionmentioning

confidence: 70%

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Cloprostenol and eCG influence oestrus synchronisation and uterine development in mice

Wei

Gong

et al. 2015

Vet. Med. - Czech

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ABSTRACT:The aim of this study was to investigate the efficacy of equine chorionic gonadotropin (eCG) and cloprostenol (CLO) administration on oestrus synchronisation, uterine development as well as serum LH and FSH concentrations in mice. One hundred and five KM mice were assigned into eCG-1, eCG-2, eCG-3, CLO-1, CLO-2, CLO-3 and control groups (CG, n = 15). The eCG-1, eCG-2 and eCG-3 groups were intramuscularly injected with 10, 20 and 40 IU eCG. CLO-1, CLO-2 and CLO-3 were intramuscularly injected with 10, 15 and 20 μg cloprostenol acetate. The results showed that 93.33% and 66.67% of synchronised mice displayed oestrus within 18.68-37.59 h. Oestrus numbers, oestrus onset time (EOT) and oestrus rate in CLO and eCG groups were greater than in CG (P < 0.05). EOT in CLO and eCG groups were 19.88 ± 2.91 h and 34.84 ± 5.05 h. Uterine weights of treatment groups were larger than CG. Uterine weights of the eCG group were higher than those in the CLO group. Uterine horn longitudial diameters (ULD) in treatment groups were larger than CG during the experiment. ULD in eCG-2 and eCG-3 were significantly greater when compared to CG (P < 0.05 or P < 0.01). On days 14 and 21, uterine horn transverse diameters (UTD) in CLO-1, eCG-1, eCG-2 and eCG-3 subgroups were significantly larger than that of CG (P < 0.05 or P < 0.01). Serum LH concentrations in eCG and CLO increased. Increments in eCG and CLO groups were greater than that of CG. FSH concentrations in eCG mice were higher than those in CLO and CG mice (P < 0.05) on day 21. Thus, eCG and cloprostenol treatments in mice can improve uterine development and promote the secretion of LH and FSH.

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“…the prooestrus, oestrus, metoestrus and dioestrus stages. The results were consistent with previous reports (Fu et al 2005;Zhu et al 2012;Gravina et al 2014). …”

Section: Discussionsupporting

confidence: 83%

Section: Discussionmentioning

confidence: 70%

Cloprostenol and eCG influence oestrus synchronisation and uterine development in mice

Wei

Gong

et al. 2015

Vet. Med. - Czech

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16S rRNA Gene Sequencing Revealed Changes in Gut Microbiota Composition during Pregnancy and Lactation in Mice Model

Guo

Cao

Zhang

et al. 2022

Veterinary Sciences

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The gut microbiota play a vital role in regulating endocrine-mediated metabolism, immunity, and energy metabolism. However, little is known about the gut microbiota and metabolite composition and development throughout pregnancy and lactation. Here, we used amplicon sequencing to analyze the gut microbiota composition of 35 female mice in five stages of pregnancy and lactation, namely, non-receptive (NR) stages, sexually-receptive (SR) stages, at day 15 of pregnancy (Pre_D15), at the day of birth (Del), and at day 10 of lactation (Lac_D10). The results revealed that the α diversity of gut microbiota was significantly increased during pregnancy and lactation. In addition, the principal coordinate analysis (PCoA) conducted on the amplicon sequence variants’ (ASVs’) distribution of the 16S rRNA amplicons indicated that the microbiota composition was significantly different among the five groups. Based on a random forest analysis, Oscillospira, Dehalobacterium, and Alistipes were the most important microbiota. The abundance of Allobaculum, Oscillospira, and Ruminococcus were negatively correlated with the serum progesterone concentration, while the abundance of Oscillospira was positively correlated with the propionate and valerate concentration in the caecal contents. Moreover, the concentration of acetate and propionate in the Del and Lac_D10 stages was significantly lower than in the SR and Pre_D15 stages. Our findings indicate that some gut microbes and metabolites vary considerably at the different stages of pregnancy and during lactation stages, which can potentially be used as microbial biomarkers. These results provide information on the potential use of the identified microbes as probiotics to maintain a healthy pregnancy and lactation.

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Measuring the estrus cycle and its effect on superovulation in mice

Cited by 2 publications

References 11 publications

Cloprostenol and eCG influence oestrus synchronisation and uterine development in mice

Cloprostenol and eCG influence oestrus synchronisation and uterine development in mice

16S rRNA Gene Sequencing Revealed Changes in Gut Microbiota Composition during Pregnancy and Lactation in Mice Model

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