How to effectively prevent land degradation and ecosystem deterioration in the process of urbanization has been the focus of land degradation researches in urban areas. Urban ecological land can be defined as the natural base on which a city relies to ecologically survive. It closely links the social economy with the natural eco‐environment, providing an important integrated approach to resolve the contradiction between urban expansion and natural ecosystems conservation in the process of urbanization. The research question addressed in this study is how to accurately identify the conservation priority areas for urban ecological land. Taking Zhuhai City, located in China, as an example, an approach based on seven kinds of water ecosystem services was put forward, combining social demand and natural supply for the services to determine service targets and conservation priority areas. The results showed that the conservation priority areas in Zhuhai City covered 868 km2, accounting for 51.03% of the total land area, which were mainly covered by woodlands or paddy fields and fish ponds. In addition, by synthesizing ecological importance and ecological sensitivity, management zones for urban ecological land were delineated, including 510 km2 of primary control areas and 358 km2 of secondary control areas. In the supply and demand view of water ecosystem services, this study put forward an integrated ecosystem‐based approach for conservation priority area identification of urban ecological land, aiming to prevent land degradation and achieve urban ecological sustainability.