Mechanical loading and the control of stem cell behavior

Manokawinchoke, Jeeranan; Pavasant, Prasit; Limjeerajarus, Chalida Nakalekha; Limjeerajarus, Nuttapol; Osathanon, Thanaphum; Egusa, Hiroshi

doi:10.1016/j.archoralbio.2021.105092

Cited by 20 publications

(15 citation statements)

References 174 publications

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“…In addition, due to the layer-by-layer curing mode of DLP 3D printing, the spiral structure with stepped characteristic similar to stairs was produced in the CL scaffolds. This “stair-like” morphology of the scaffolds can mediate the actin, vinculin, and fibronectin expression of loaded cells, thus affecting their adhesion and proliferation ability on scaffolds [ 38 , [51] , [52] , [53] , [54] ]. Overall, these results demonstrated the improved cell delivery efficiency of the CL scaffolds.…”

Section: Resultsmentioning

confidence: 99%

“…Previous studies found that surface topography could regulate osteogenic differentiation of MSCs via crosstalk between FAK/MAPK and ILK/ β -catenin pathways [ 61 ]. In addition, the macro-microstructure would influence the tension of intracellular skeleton, and then activate signaling pathways such as Runx2, Wnt, to stimulate osteogenic differentiation [ 49 , 53 , 55 , 62 ].…”

Section: Resultsmentioning

confidence: 99%

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3D printing of conch-like scaffolds for guiding cell migration and directional bone growth

Feng

Zhang

Chen

et al. 2023

Bioactive Materials

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Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

3D printing of conch-like scaffolds for guiding cell migration and directional bone growth

Feng

Zhang

Chen

et al. 2023

Bioactive Materials

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“…Several studies have demonstrated that mechanical forces induce the differentiation and maturation of stem cells toward bone 4,24,25 and cartilage cells 11,[26][27][28] . Laminar shear stress mediates early lineage specification of singlecell monolayer-cultured mouse ES cells 6 .…”

Section: Discussionmentioning

confidence: 99%

Application of shear stress for enhanced osteogenic differentiation of mouse induced pluripotent stem cells

Limraksasin

Nattasit

Manokawinchoke

et al. 2022

Sci Rep

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The self-organizing potential of induced pluripotent stem cells (iPSCs) represents a promising tool for bone tissue engineering. Shear stress promotes the osteogenic differentiation of mesenchymal stem cells, leading us to hypothesize that specific shear stress could enhance the osteogenic differentiation of iPSCs. For osteogenesis, embryoid bodies were formed for two days and then maintained in medium supplemented with retinoic acid for three days, followed by adherent culture in osteogenic induction medium for one day. The cells were then subjected to shear loading (0.15, 0.5, or 1.5 Pa) for two days. Among different magnitudes tested, 0.5 Pa induced the highest levels of osteogenic gene expression and greatest mineral deposition, corresponding to upregulated connexin 43 (Cx43) and phosphorylated Erk1/2 expression. Erk1/2 inhibition during shear loading resulted in decreased osteogenic gene expression and the suppression of mineral deposition. These results suggest that shear stress (0.5 Pa) enhances the osteogenic differentiation of iPSCs, partly through Cx43 and Erk1/2 signaling. Our findings shed light on the application of shear-stress technology to improve iPSC-based tissue-engineered bone for regenerative bone therapy.

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“…5 The effect of biophysical forces on the JAK/STAT pathway \Cells can sense their extracellular environment and respond to chemical (Armstrong et al, 2017), optical (Pail et al, 2011), thermal (McGlynn et al, 2021, and biophysical forces (Pan et al, 1999;Ruwhof and van der Laarse, 2000) signals, which can affect downstream cellular signaling pathways via second messenger cascades (Pan et al, 1999). Ultimately, these changes alter cell behavior and functions (Komuro et al, 1990;Sadoshima and Izumo, 1993;Manokawinchoke et al, 2021). Unlike the effects of drug stimulation or gene editing on cellular activity and pharmacological responses (Bhardwaj et al, 2014), cells are often subjected to continuous and weak mechanical forces from the surroundings (Wang et al, 2010;Wang et al, 2016;Ding et al, 2017).…”

Section: Other Cytokines In Jak/stat Pathwaymentioning

confidence: 99%

JAK/STAT pathway: Extracellular signals, diseases, immunity, and therapeutic regimens

Bian

Rong

et al. 2023

Front. Bioeng. Biotechnol.

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Janus kinase/signal transduction and transcription activation (JAK/STAT) pathways were originally thought to be intracellular signaling pathways that mediate cytokine signals in mammals. Existing studies show that the JAK/STAT pathway regulates the downstream signaling of numerous membrane proteins such as such as G-protein-associated receptors, integrins and so on. Mounting evidence shows that the JAK/STAT pathways play an important role in human disease pathology and pharmacological mechanism. The JAK/STAT pathways are related to aspects of all aspects of the immune system function, such as fighting infection, maintaining immune tolerance, strengthening barrier function, and cancer prevention, which are all important factors involved in immune response. In addition, the JAK/STAT pathways play an important role in extracellular mechanistic signaling and might be an important mediator of mechanistic signals that influence disease progression, immune environment. Therefore, it is important to understand the mechanism of the JAK/STAT pathways, which provides ideas for us to design more drugs targeting diseases based on the JAK/STAT pathway. In this review, we discuss the role of the JAK/STAT pathway in mechanistic signaling, disease progression, immune environment, and therapeutic targets.

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Mechanical loading and the control of stem cell behavior

Cited by 20 publications

References 174 publications

3D printing of conch-like scaffolds for guiding cell migration and directional bone growth

3D printing of conch-like scaffolds for guiding cell migration and directional bone growth

Application of shear stress for enhanced osteogenic differentiation of mouse induced pluripotent stem cells

JAK/STAT pathway: Extracellular signals, diseases, immunity, and therapeutic regimens

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