Mechanical properties and cellular content of leukocyte‐ and platelet‐rich fibrin membranes of patients on antithrombotic drugs

Ockerman, Anna; Hendrickx, Amber; Willekens, Wouter; Fehervary, Heleen; Vastmans, Julie; Coucke, Wim; Verhamme, Peter; Politis, Constantinus; Vanassche, Thomas; Braem, Annabel; Quirynen, Marc; Jacobs, Reinhilde

doi:10.1111/jre.12991

Cited by 6 publications

(4 citation statements)

References 29 publications

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“…This may suggest that for a certain treatment or application in the clinic, different responses can be expected between different patients and that maybe not all patients would benefit in the same way from L-PRF treatment. It is known already that the patient’s medical history, such as diabetes and the use of anticoagulant treatment, can influence the mechanical and healing properties of L-PRF [ 72 ].…”

Section: Discussionmentioning

confidence: 99%

The Effect of Leukocyte- and Platelet-Rich Fibrin on Central and Peripheral Nervous System Neurons—Implications for Biomaterial Applicability

Lambrichts,

Wolfs,

Bronckaers

et al. 2023

IJMS

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Leukocyte- and Platelet-Rich Fibrin (L-PRF) is a second-generation platelet concentrate that is prepared directly from the patient’s own blood. It is widely used in the field of regenerative medicine, and to better understand its clinical applicability we aimed to further explore the biological properties and effects of L-PRF on cells from the central and peripheral nervous system. To this end, L-PRF was prepared from healthy human donors, and confocal, transmission, and scanning electron microscopy as well as secretome analysis were performed on these clots. In addition, functional assays were completed to determine the effect of L-PRF on neural stem cells (NSCs), primary cortical neurons (pCNs), and peripheral dorsal root ganglion (DRG) neurons. We observed that L-PRF consists of a dense but porous fibrin network, containing leukocytes and aggregates of activated platelets that are distributed throughout the clot. Antibody array and ELISA confirmed that it is a reservoir for a plethora of growth factors. Key molecules that are known to have an effect on neuronal cell functions such as brain-derived neurotrophic factor (BDNF), nerve growth factor (NGF), vascular endothelial growth factor (VEGF), and platelet-derived growth factor (PDGF) were slowly released over time from the clots. Next, we found that the L-PRF secretome had no significant effect on the proliferative and metabolic activity of NSCs, but it did act as a chemoattractant and improved the migration of these CNS-derived stem cells. More importantly, L-PRF growth factors had a detrimental effect on the survival of pCNs, and consequently, also interfered with their neurite outgrowth. In contrast, we found a positive effect on peripheral DRG neurons, and L-PRF growth factors improved their survival and significantly stimulated the outgrowth and branching of their neurites. Taken together, our study demonstrates the positive effects of the L-PRF secretome on peripheral neurons and supports its use in regenerative medicine but care should be taken when using it for CNS applications.

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Section: Discussionmentioning

confidence: 99%

The Effect of Leukocyte- and Platelet-Rich Fibrin on Central and Peripheral Nervous System Neurons—Implications for Biomaterial Applicability

Lambrichts,

Wolfs,

Bronckaers

et al. 2023

IJMS

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“…32,33 Lastly, due to the nature of autologous materials, there may be variability in materials produced based upon patient-related factors. 34,35 Given these caveats, patient and case selection are important when considering the use of adjunctive ABPs.…”

Section: Discussionmentioning

confidence: 99%

“…Depending upon the materials and/or system used, there may be variability in preparation protocols and limited evidence exists regarding the validation of some materials and protocols 32,33 . Lastly, due to the nature of autologous materials, there may be variability in materials produced based upon patient‐related factors 34,35 . Given these caveats, patient and case selection are important when considering the use of adjunctive ABPs.…”

Section: Discussionmentioning

confidence: 99%

Clinical applications of mucogingival therapies utilizing adjunctive autologous blood products

Geisinger

Kaur

Arraj

et al. 2022

Clin Adv Periodontics

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Background Mucogingival deformities are prevalent oral conditions and can result in esthetic compromise, dentinal hypersensitivity, and an increase in radicular caries rates. Mucogingival treatments address thin periodontal phenotype and/or gingival recession defects. Although many of these grafting procedures are predictable in improving soft‐tissue quality and quantity around teeth and dental implants, patients often complain of discomfort at both the donor and recipient sites. Free gingival grafts and coronally advanced flaps alone or in combination with subepithelial connective tissue graft and/or acellular dermal matrix are among the most common surgical procedures employed to achieve root coverage and enhance periodontal phenotype. Autologous blood products (ABPs) contain platelets, growth factors, leukocytes, and stem cells that contribute to cell mitosis, collagen production, and angiogenesis, leading to the healing and regeneration of hard and soft tissue. Evaluation of the adjunctive role of ABPs in mucogingival surgery and their impacts on clinical and patient‐centered data is critical to achieve optimal patient‐reported outcome measures based upon the current scientific evidence. Methods We present exemplar cases in which adjunctive ABPs were utilized in mucogingival treatment to enhance treatment outcomes. Results No adverse events were noted. Satisfactory treatment outcomes were achieved in patients with local and/or systemic compromise when mucogingival therapies were used in combination with ABPs. Conclusions Adjunctive use of ABPs may enhance outcomes of mucogingival therapy. Utilization of adjunctive ABPs may be particularly advantageous in situations where the predictability of clinical and esthetic outcomes is limited due to anatomical and/or patient factors.

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“…However, these regenerative therapeutic approaches, without the use of supporting osteoconductive materials, have been shown to have some limitations in inducing true periodontal regeneration with the formation of new tissue. Specifically, through investigation methods with the use of tissue engineering, various methods of periodontal regeneration were studied with the combination of bone grafts, growth factors, 3D bone scaffolds and amelogenins, to be used in regenerative periodontal surgery with and without the use of membranes [ 12 ]. To this end, both guided tissue regeneration (GTR) and guided bone regeneration (GBR) [ 13 ] have been introduced to properly support tissue healing mechanisms with or without the use of biomaterials.…”

mentioning

confidence: 99%

Advances in Clinical and Molecular Research of Biomaterials in Dentistry: The New Era for Dental Applications

Isola

Lombardi²

2022

JCM

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Mechanical properties and cellular content of leukocyte‐ and platelet‐rich fibrin membranes of patients on antithrombotic drugs

Cited by 6 publications

References 29 publications

The Effect of Leukocyte- and Platelet-Rich Fibrin on Central and Peripheral Nervous System Neurons—Implications for Biomaterial Applicability

The Effect of Leukocyte- and Platelet-Rich Fibrin on Central and Peripheral Nervous System Neurons—Implications for Biomaterial Applicability

Clinical applications of mucogingival therapies utilizing adjunctive autologous blood products

Advances in Clinical and Molecular Research of Biomaterials in Dentistry: The New Era for Dental Applications

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