Mechanism of melatonin combined with calcium carbonate on improving osteoporosis in aged rats

Zhu, Fuqiang; Liu, Zhendong; Ren, Yi

doi:10.3892/etm.2018.6141

Cited by 7 publications

(5 citation statements)

References 27 publications

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“…The indole hormone MLT influences bone metabolism by promoting bone matrix mineralization through the modulation of different signal transduction pathways . Previous studies, mainly carried out in animal models of osteoporosis, examined the safety and efficacy of MLT administered with different strategies and dosages.…”

Section: Discussionsupporting

confidence: 52%

“…43,61 The indole hormone MLT 26 influences bone metabolism by promoting bone matrix mineralization through the modulation of different signal transduction pathways. 62 Previous studies, mainly carried out in animal models of osteoporosis, examined the safety and efficacy of MLT administered with different strategies and dosages. Ladizesky et al 63 demonstrated an increase in bone alkaline phosphatase in ovariectomized rats receiving 25 µg/mL of MLT in drinking water compared with control rats.…”

Section: Discussionmentioning

confidence: 99%

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Melatonin prevents cadmium‐induced bone damage: First evidence on an improved osteogenic/adipogenic differentiation balance of mesenchymal stem cells as underlying mechanism

Knani

Bartolini

Kechiche

et al. 2019

Journal of Pineal Research

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Melatonin (MLT) plays a role in preserving bone health, a function that may depend on homeostatic effects on both mature osteoblasts and mesenchymal stem cells (MSCs) of the bone tissue. In this study, these functions of MLT have been investigated in rat bone (femur) and in human adipose MSC (hMSC) during chronic exposure to low‐grade cadmium (Cd) toxicity, a serious public health concern. The in vivo findings demonstrate that MLT protects against Cd‐induced bone metabolism disruption and accumulation of bone marrow adipocytes, a cue of impaired osteogenic potential of skeletal MSC niches. This latter symptom was recapitulated in hMSCs in which Cd toxicity stimulated adipogenic differentiation. MLT was found to rescue, at least in part, the osteogenic differentiation properties of these cells. This study reports on a new bone cytoprotection function of MLT pertinent to Cd toxicity and its interfering effect on skeletal MSC differentiation properties that is worth investigating for its possible impact on human bone pathophysiology.

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Section: Discussionsupporting

confidence: 52%

Section: Discussionmentioning

confidence: 99%

Melatonin prevents cadmium‐induced bone damage: First evidence on an improved osteogenic/adipogenic differentiation balance of mesenchymal stem cells as underlying mechanism

Knani

Bartolini

Kechiche

et al. 2019

Journal of Pineal Research

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“…NAC was injected once every 2 weeks for a total of 5 times. The dosage of TFRD and CaCO 3 was selected based on the research reports of Fang et al (21) and Zhu et al (22).…”

Section: Experimental Animalsmentioning

confidence: 99%

Total flavonoids of Rhizoma Drynariae combined with calcium attenuate osteoporosis by reducing reactive oxygen species generation

et al. 2021

Exp Ther Med

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In the present study, the effects of total flavonoids of Rhizoma Drynariae (TFRD) and calcium carbonate (CaCO 3 ) on osteoporosis (OP) were assessed in a rat model of OP. For this purpose, 36 Sprague-Dawley rats, aged 3 months, were randomly divided into a group undergoing sham surgery (sham-operated group), model group (OP group), CaCO 3 group (OP + CaCO 3 group), TFRD group (OP + TFRD group), TFRD combined with CaCO 3 group (OP + TFRD + CaCO 3 group) and TFRD and CaCO 3 combined with N-acetyl cysteine group (OP + TFRD + CaCO 3 + NAC group). The rat model of OP was established by bilateral ovariectomy. The changes in bone mineral density (BMD), bone volume parameters and bone histopathology in the rats from each group were observed. The levels of serum reactive oxygen species, superoxide dismutase (SOD), malondialdehyde, glutathione peroxidase (GSH-Px), interleukin (IL)-6, IL-1β, TNF-α, and the levels of bone tissue runt-related transcription factor 2 (RUNX2), osteoprotegerin (OPG), osteocalcin (BGP), PI3K, p-PI3K, AKT, p-AKT, mammalian target of rapamycin (mTOR) and p-mTOR were measured in the rats of each group. The induction of OP was associated with a marked decrease in BMD, bone mineral content, bone volume fraction and trabecular thickness, and decreased serum levels of SOD and GSH-Px. Moreover, the expressions of RUNX2, OPG, BGP were downregulated and an upregulation of p-PI3K, p-AKT and p-mTOR were observed in osteoporotic rats. However, treatment with TFRD and CaCO 3 restored all the aforementioned parameters to almost normal values. Furthermore, the findings on histopathological evaluation were consistent with the biochemical observations. Taken together, the findings of the present study demonstrated that TFRD and CaCO 3 significantly increased the antioxidant capacity in rats with OP, increased BMD and reduced bone mineral loss, and may be useful for the prevention and treatment of OP.

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“…Our research also demonstrated that MT can enhance bone microstructure and bone metabolism in rodents with oophorectomized osteoporosis. Despite the fact that numerous studies ( 29 – 32 ) suggest MT can reduce the harm caused by oxidative stress and inflammation to osteoblasts, as well as ward off osteolysis caused by reactive oxygen species and inflammatory factors, we still believe MT can be beneficial for the health of bone structure through its control of bone microenvironment and metabolism. Nevertheless, further research is needed for its clinical application in the prevention and treatment of osteoporosis.…”

Section: Discussionmentioning

confidence: 94%

Leptin and melatonin’s effects on OVX rodents’ bone metabolism

Lin

Xiong

et al. 2023

Front. Endocrinol.

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PurposeThis study aims to examine the effects of leptin and melatonin intervention on bone metabolism in ovariectomize (OVX) rodents, as well as their potential mechanisms of action.MethodsPrepare an OVX model of osteoporosis in rodents and validate the model by collecting bilateral tibia samples for Micro-CT scanning and histological analysis. A control group of normal size, the OVX group, the OVX+Sema4D (Semaphorin 4D) group, the OVX+Sema4D+Leptin group, the OVX+Sema4D+ Melatonin(MT) group and the OVX+Sema4D+Leptin+ MT group were the experimental groups. Adenovirus vector construction and tibial medullary injection validation were conducted in accordance with the aforementioned experimental groups. Four groups of rats were injected with the Sema4D overexpression adenovirus vector into the tibial medullary cavity, and two groups were injected with the Leptin overexpression adenovirus vector. The repair of osteoporosis was observed using micro-CT and histological analysis. Immunohistochemical detection of bone morphogenetic protein-2 (BMP-2) expression in bone tissue was employed to ascertain the amount of osteoclasts in the upper tibial metaphysis, utilizing TRAP(tartrate-resistant acid phosphatase) staining.ResultsIncreased levels of BV/TV, Tb.N, BMD, and BMC were seen in the OVX+ Sema4D+Leptin, OVX+ Sema4D+MT, and OVX+ Sema4D+Leptin+ MT groups compared to the OVX group, whereas Tb. Sp levels were lowered. When compared to the Sema4D overexpression group, the trabecular bone structure of the OVX + Sema4D + Leptin, OVX + Sema4D + MT, and OVX + Sema4D + Leptin + MT groups is largely intact, tends to be closer, and the amount of trabecular bone increases. The OVX + Sema4D + Leptin + MT group in particular.The expression of BMP-2 was dramatically upregulated (p<0.05), the number of TRAP-stained osteoclasts was significantly reduced (p<0.05), and BALP(bone-derived alkaline phosphatase) and TRAP-5b(tartrate-resistant acid phosphatase-5b) activities were significantly downregulated (p<0.05).ConclusionIn rats with osteoporosis, leptin and melatonin can be seen to augment the trabecular microstructure of the bone, augment bone growth, diminish trabecular harm, and mend the bone. The combined effect is more powerful.

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Mechanism of melatonin combined with calcium carbonate on improving osteoporosis in aged rats

Cited by 7 publications

References 27 publications

Melatonin prevents cadmium‐induced bone damage: First evidence on an improved osteogenic/adipogenic differentiation balance of mesenchymal stem cells as underlying mechanism

Melatonin prevents cadmium‐induced bone damage: First evidence on an improved osteogenic/adipogenic differentiation balance of mesenchymal stem cells as underlying mechanism

Total flavonoids of Rhizoma Drynariae combined with calcium attenuate osteoporosis by reducing reactive oxygen species generation

Leptin and melatonin’s effects on OVX rodents’ bone metabolism

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