Mechanisms of carbon nanotube-induced toxicity: Focus on oxidative stress

Shvedova, Anna A.; Pietroiusti, Antonio; Fadeel, Bengt; Kagan, Valerian E.

doi:10.1016/j.taap.2012.03.023

Cited by 451 publications

(302 citation statements)

References 145 publications

(148 reference statements)

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“…6 CBNs can exert toxic effects at the cellular level, primarily via generation of reactive oxygen species (ROS) and subsequent cellular oxidative stress. 7 Studies have shown that their ability to aggregate and physical contact of these particles with cell membranes plays a role in this toxicity. 8,9 In addition, due to methods of manufacture, there are often metal impurities (remnants of catalysis) that can cause adverse effects.…”

Section: Introductionmentioning

confidence: 99%

Mid-infrared spectroscopic assessment of nanotoxicity in Gram-negative vs. Gram-positive bacteria

Heys

Riding

Strong

et al. 2014

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Vibrational spectroscopy provides a spectral fingerprint identifying the effects of carbonbased nanoparticles in bacterial cells. 3 ABSTRACTNanoparticles appear to induce toxic effects through a variety of mechanisms including generation of reactive oxygen species (ROS), physical contact with the cell membrane and indirect catalysis due to remnants from manufacture. The development and subsequent increasing usage of nanomaterials has highlighted a growing need to characterize and assess the toxicity of nanoparticles, particularly those that may have detrimental health effects such as carbon-based nanomaterials (CBNs). Due to interactions of nanoparticles with some reagents, many traditional toxicity tests are unsuitable for use with CBNs. Infrared (IR) spectroscopy is a non-destructive, high throughput technique, which is unhindered by such problems. We explored the application of IR spectroscopy to investigate the effects of CBNs on Gram-negative (Pseudomonas fluorescens) and Gram-positive (Mycobacterium vanbaalenii PYR-1) bacteria. Two types of IR spectroscopy were compared: attenuated total reflection Fourier-transform infrared (ATR-FTIR) and synchrotron radiation-based FTIR (SR-FTIR) spectroscopy. This showed that Gram-positive and Gram-negative bacteria exhibit differing alterations when exposed to CBNs. Gram-positive bacteria appear more resistant to these agents and this may be due to the protection afforded by their more sturdy cell wall. Markers of exposure also vary according to Gram status; Amide II was consistently altered in Gram-negative bacteria and carbohydrate altered in Gram-positive bacteria. ATR-FTIR and SR-FTIR spectroscopy could both be applied to extract biochemical alterations induced by each CBN that were consistent across the two bacterial species; these may represent potential biomarkers of nanoparticle-induced alterations. Vibrational spectroscopy approaches may provide a novel means of fingerprinting the effects of CBNs in target cells.

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Section: Introductionmentioning

confidence: 99%

Mid-infrared spectroscopic assessment of nanotoxicity in Gram-negative vs. Gram-positive bacteria

Heys

Riding

Strong

et al. 2014

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“…However, it is also important to assess whether "oxidative stress" is merely a secondary event resulting inevitably from disruption of biochemical processes and the demise of the cell, or a specific, non-random event that plays a role in the induction of cellular damage, e.g., apoptosis? For a further discussion of these issues, readers are referred to Shedova et al [108]. The mechanism by which oxidative damage occurs appears to be via the inhibition of electron transfer in the TCA (tricarboxylic acid) cycle and the consequent accumulation of TCA cycle intermediates.…”

Section: The Signalling Concept: Interaction Of Nanoparticles With Mamentioning

confidence: 99%

The bio-nano-interface in predicting nanoparticle fate and behaviour in living organisms: towards grouping and categorising nanomaterials and ensuring nanosafety by design

et al. 2013

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Abstract:In biological media, nanoparticles acquire a coating of biomolecules (proteins, lipids, polysaccharides) from their surroundings, which reduces their surface energy and confers a biological identity to the particles. This adsorbed layer is the interface between the nanomaterial and living systems and therefore plays a significant role in determining the fate and behaviour of the nanoparticles. This review summarises the state of the art in terms of understanding the bio-nano interface and provides direction for potential future research and recommendations for future priorities and strategies to support the safe implementation of nanotechnologies. The central premise is that nanomaterials must be studied as biological entities under the appropriate exposure conditions and that this should be implemented in study design and reporting for nanosafety assessment. The implications of the bio-nano interface for nanomaterials fate and behaviour are described in light of four interlinked perspectives: the Coating concept; the Translocation concept; the Signalling concept, and the Kinetics concept. A key conclusion is that nanoparticles cannot be viewed as non-interacting species, but rather must be thought of, and studied as, biological entities, where their interaction with the environment is mediated by the proteins and other biomolecules that adsorb to them, and the key parameter to characterise then becomes the nature, composition and evolution of the bionano interface.

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“…[16][17][18][19] CNTs can elicit toxicity through membrane damage, DNA damage, oxidative stress, changes in mitochondrial activities, altered intracellular metabolic routes, and protein synthesis. 10,[20][21][22][23][24] The most common mechanisms of CNT cytotoxicity also encompass apoptosis and necrosis. [25][26][27] However, CNT cytotoxicity is significantly controversial, with a large number of studies reporting altered toxic responses to CNTs both in vitro and in vivo.…”

Section: Toxicity Of Carbon Nanotubesmentioning

confidence: 99%

Functionalized carbon nanotubes: biomedical applications

Vardharajula¹,

Ali²,

Tiwari³

et al. 2012

IJN

184

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Carbon nanotubes (CNTs) are emerging as novel nanomaterials for various biomedical applications. CNTs can be used to deliver a variety of therapeutic agents, including biomolecules, to the target disease sites. In addition, their unparalleled optical and electrical properties make them excellent candidates for bioimaging and other biomedical applications. However, the high cytotoxicity of CNTs limits their use in humans and many biological systems. The biocompatibility and low cytotoxicity of CNTs are attributed to size, dose, duration, testing systems, and surface functionalization. The functionalization of CNTs improves their solubility and biocompatibility and alters their cellular interaction pathways, resulting in muchreduced cytotoxic effects. Functionalized CNTs are promising novel materials for a variety of biomedical applications. These potential applications are particularly enhanced by their ability to penetrate biological membranes with relatively low cytotoxicity. This review is directed towards the overview of CNTs and their functionalization for biomedical applications with minimal cytotoxicity.

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Mechanisms of carbon nanotube-induced toxicity: Focus on oxidative stress

Cited by 451 publications

References 145 publications

Mid-infrared spectroscopic assessment of nanotoxicity in Gram-negative vs. Gram-positive bacteria

Mid-infrared spectroscopic assessment of nanotoxicity in Gram-negative vs. Gram-positive bacteria

The bio-nano-interface in predicting nanoparticle fate and behaviour in living organisms: towards grouping and categorising nanomaterials and ensuring nanosafety by design

Functionalized carbon nanotubes: biomedical applications

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