Mechanisms of structure generation during plastic compression of nanofibrillar collagen hydrogel scaffolds: towards engineering of collagen

Hadjipanayi, Ektoras; Ananta, M.; Binkowski, Marcin; Streeter, Ian; Lu, Zufu; Cui, Zhanfeng; Brown, Robert A.; Mudera, Vivek

doi:10.1002/term.343

Cited by 35 publications

(22 citation statements)

References 58 publications

(88 reference statements)

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“…20,21 In this study, cells are in contact with the FLS of the CC constructs and the nFLS of the UC constructs. It is well known that soluble factors, cell-extracellular matrix interactions as well as external mechanical loads influence cell proliferation and differentiation.…”

Section: Discussionmentioning

confidence: 99%

Plastic Compressed Collagen Constructs for Ocular Cell Culture and Transplantation: A New and Improved Technique of Confined Fluid Loss

Levis¹,

Menzel‐Severing²,

Drake³

et al. 2012

Current Eye Research

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This modification allows scaling up of the process in a well-plate format, which is essential for creation of multiple corneal epithelial models for in vitro testing. This improvement to the original plastic compression method also allows the process to be employed in custom-made cassettes, the design of which takes into consideration the manufacturing and regulatory requirements for delivery of a cell therapy.

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Section: Discussionmentioning

confidence: 99%

Plastic Compressed Collagen Constructs for Ocular Cell Culture and Transplantation: A New and Improved Technique of Confined Fluid Loss

Levis¹,

Menzel‐Severing²,

Drake³

et al. 2012

Current Eye Research

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show abstract

“…37 It has also been reported that the formation of a dense collagen region during plastic compression of collagen gels has increased hydraulic resistance of hydrated gels, as well as diminishing the fluid flow through the construct. 38,39 In addition, after transplantation, the bladder could have a tube for drainage during the time of healing. Risk for intraperitoneal leakage is then small.…”

Section: One-stage Tissue Engineering Of Bladder Wall Patchesmentioning

confidence: 99%

One-Stage Tissue Engineering of Bladder Wall Patches for an Easy-To-Use Approach at the Surgical Table

Ajalloueian

Zeiai²,

Rojas³

et al. 2013

Tissue Engineering Part C: Methods

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We present a method for producing a cell-scaffold hybrid construct at the bedside. The construct is composed of plastic-compressed collagen together with a poly(ɛ-caprolactone) (PCL)-knitted mesh that yields an integrated, natural-synthetic scaffold. This construct was evaluated by seeding of minced bladder mucosa, followed by proliferation in vitro. High mechanical strength in combination with a biological environment suitable for tissue growth was achieved through the creation of a hybrid construct that showed an increased tensile strength (17.9 ± 2.6 MPa) when compared to plastic-compressed collagen (0.6 ± 0.12 MPa). Intimate contact between the collagen and the PCL fabric was required to ensure integrity without delamination of the construct. This contact was achieved by surface alkaline hydrolysis of the PCL, followed by adsorption of poly(vinyl) alcohol. The improvement in hydrophilicity of the PCL-knitted mesh was confirmed through water contact angle measurements, and penetration of the collagen into the mesh was evaluated by scanning electron microscopy (SEM). Particles of minced bladder mucosa tissue were seeded onto this scaffold, and the proliferation was followed for 6 weeks in vitro. Results obtained from phase contrast microscopy, SEM, and histological staining indicated that cells migrated from the minced tissue particles and reorganized on the scaffold. Cells were viable and proliferative, with morphological features characteristic of urothelial cells. Proliferation reached the point at which a multilayer with a resemblance to stratified urothelium was achieved. This successful method could potentially be used for in vivo applications in reconstructive urology as an engineered autologous tissue transplant without the requirement for in vitro culture before transplantation.

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“…In addition, other techniques such as plastic compression could also be applied to reduce water content and increase the overall material stiffness. [55][56][57] …”

Section: Ahearne and Lynchmentioning

confidence: 99%

Early Observation of Extracellular Matrix-Derived Hydrogels for Corneal Stroma Regeneration

Ahearne

Lynch

2015

Tissue Engineering Part C: Methods

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A lack of healthy transplantable tissue to treat corneal blindness has led researchers to investigate the development and application of different scaffold materials for corneal tissue engineering and regeneration. In this study, hydrogels fabricated from decellularized corneal extracellular matrix were developed as a new approach to corneal stromal tissue regeneration. Porcine corneas were decellularized using a technique that combined freeze-thaw cycles with a nuclease treatment. The corneas were then freeze-dried, milled, and digested in an acidic pepsin solution that was used to form a hydrogel after adjusting the pH and gelation temperature. The resultant corneal matrix hydrogels (CMHs) were seeded with human corneal stromal cells and cultured for several days. When compared to collagens hydrogels, CMHs had superior optical transparency, similar mechanical properties, and were better able to retain the stromal cells native keratocyte phenotype. The CMHs also supported cell viability and proliferation and contained sulfated glycosaminoglycan, a vital constituent, for maintaining corneal transparency. These results suggest that the CMHs could provide an exceptional biomaterial for corneal stroma regeneration.

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Mechanisms of structure generation during plastic compression of nanofibrillar collagen hydrogel scaffolds: towards engineering of collagen

Cited by 35 publications

References 58 publications

Plastic Compressed Collagen Constructs for Ocular Cell Culture and Transplantation: A New and Improved Technique of Confined Fluid Loss

Plastic Compressed Collagen Constructs for Ocular Cell Culture and Transplantation: A New and Improved Technique of Confined Fluid Loss

One-Stage Tissue Engineering of Bladder Wall Patches for an Easy-To-Use Approach at the Surgical Table

Early Observation of Extracellular Matrix-Derived Hydrogels for Corneal Stroma Regeneration

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