Mechanistic aspects of protein corona formation: insulin adsorption onto gold nanoparticle surfaces

Graß, Stefan; Treuel, Lennart

doi:10.1007/s11051-014-2254-0

Cited by 18 publications

(37 citation statements)

References 90 publications

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“…Polymer coatings are frequently stable under such conditions but other ligands can well be replaced in equilibrium-type reactions even under chemically less complex conditions [54,71]. …”

Section: Reviewmentioning

confidence: 99%

PVP-coated, negatively charged silver nanoparticles: A multi-center study of their physicochemical characteristics, cell culture and in vivo experiments

Ahlberg¹,

Antonopulos²,

Diendorf³

et al. 2014

Beilstein J. Nanotechnol.

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122

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SummaryPVP-capped silver nanoparticles with a diameter of the metallic core of 70 nm, a hydrodynamic diameter of 120 nm and a zeta potential of −20 mV were prepared and investigated with regard to their biological activity. This review summarizes the physicochemical properties (dissolution, protein adsorption, dispersability) of these nanoparticles and the cellular consequences of the exposure of a broad range of biological test systems to this defined type of silver nanoparticles. Silver nanoparticles dissolve in water in the presence of oxygen. In addition, in biological media (i.e., in the presence of proteins) the surface of silver nanoparticles is rapidly coated by a protein corona that influences their physicochemical and biological properties including cellular uptake. Silver nanoparticles are taken up by cell-type specific endocytosis pathways as demonstrated for hMSC, primary T-cells, primary monocytes, and astrocytes. A visualization of particles inside cells is possible by X-ray microscopy, fluorescence microscopy, and combined FIB/SEM analysis. By staining organelles, their localization inside the cell can be additionally determined. While primary brain astrocytes are shown to be fairly tolerant toward silver nanoparticles, silver nanoparticles induce the formation of DNA double-strand-breaks (DSB) and lead to chromosomal aberrations and sister-chromatid exchanges in Chinese hamster fibroblast cell lines (CHO9, K1, V79B). An exposure of rats to silver nanoparticles in vivo induced a moderate pulmonary toxicity, however, only at rather high concentrations. The same was found in precision-cut lung slices of rats in which silver nanoparticles remained mainly at the tissue surface. In a human 3D triple-cell culture model consisting of three cell types (alveolar epithelial cells, macrophages, and dendritic cells), adverse effects were also only found at high silver concentrations. The silver ions that are released from silver nanoparticles may be harmful to skin with disrupted barrier (e.g., wounds) and induce oxidative stress in skin cells (HaCaT). In conclusion, the data obtained on the effects of this well-defined type of silver nanoparticles on various biological systems clearly demonstrate that cell-type specific properties as well as experimental conditions determine the biocompatibility of and the cellular responses to an exposure with silver nanoparticles.

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“…Polymer coatings are frequently stable under such conditions but other ligands can well be replaced in equilibrium-type reactions even under chemically less complex conditions [54,71]. …”

Section: Reviewmentioning

confidence: 99%

PVP-coated, negatively charged silver nanoparticles: A multi-center study of their physicochemical characteristics, cell culture and in vivo experiments

Ahlberg¹,

Antonopulos²,

Diendorf³

et al. 2014

Beilstein J. Nanotechnol.

Self Cite

122

View full text Add to dashboard Cite

show abstract

“…The Raman signal produces a precise spectral sample fingerprint, unique to each atom, group of atoms or individual molecule (Kengne-Momo et al 2012). A recent study has used surface enhanced Raman scattering spectroscopy to study changes in the proteins secondary structure as well as the effect on integrity and conformations of disulfide bonds immediately on the NP surface (Grass and Treuel 2014).…”

Section: Raman Spectroscopymentioning

confidence: 99%

Cellular Response of Therapeutic Nanoparticles

Kumari

Singla

Guliani

et al. 2016

Nanoscale Materials in Targeted Drug Delivery, Theragnosis and Tissue Regeneration

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Nanoparticles (NPs) are being extensively used in the field of nanomedicines. Different types of NPs are administered into the body by various routes. NPs come in contact with cells inside the body. Cellular response of NPs is affected by size, shape, surface chemistry, and cellular uptake pathways of NPs. In addition to this, type of cells, various cell lines, and growth media are also found to affect the cellular response of NPs. NPs induce diverse cellular responses like apoptosis, necrosis, and reactive oxygen species (ROS) production. NPs also form a protein corona inside the biological media which may alter their identity and behaviour as compared to bare NPs. In this chapter, we have made an attempt to throw light on cellular uptake pathways of NPs, monitoring of endocytic pathways followed by NPs, factors affecting cellular responses of therapeutic NPs, and protein corona formation, characterisation and its implications on fate of NPs.

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“…19 With the development of nanotechnology, gold nanoparticles were introduced to adsorb insulin as well. 20 Adsorption of insulin with porous hydroxyapatite microspheres also obtained good results. 21 In the meantime, other researchers studied the interactions between insulin and the adsorption medium using new technologies, such as label-free electroanalytical, circular dichroism (CD) and isothermal titration calorimetry.…”

Section: Introductionmentioning

confidence: 95%

“…Polyglycidyl methacrylate containing immobilized anti‐insulin antibodies was used as an adsorption sorbent to separate insulin . With the development of nanotechnology, gold nanoparticles were introduced to adsorb insulin as well . Adsorption of insulin with porous hydroxyapatite microspheres also obtained good results .…”

Section: Introductionmentioning

confidence: 99%

Preparation of pH-responsive metal chelate affinity polymer for adsorption and desorption of insulin

Ding

Lin

Liu

et al. 2016

J. Chem. Technol. Biotechnol

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BACKGROUND Insulin is a medicine for diabetes mellitus that is used worldwide. This study focuses on the preparation of a pH‐responsive metal chelated affinity polymer and the adsorption of insulin by this polymer. RESULTS The pH‐responsive polymer was synthesized and zinc ions were attached to the polymers as the affinity polymer PMMDN‐Zn2+, which can adsorb insulin. The molecular weight, isoelectric point (pI) which is the pH at which the net charge on the polymer is zero, and other characteristics of the polymers were determined. Circular dichroism spectroscopy was performed to analyze the interaction between the metal ions and insulin. The optimum adsorption conditions, including the time, pH, salt concentrations and insulin concentrations were investigated. The pI of the polymer was 3.41 and the recovery of the polymer was 97.5%. The maximum adsorption capacity of PMMDN‐Zn2+ towards insulin was 162.3 mg insulin g−1 polymer. CONCLUSION This results suggest that the affinity polymer PMMDN‐Zn2+ has potential for application on a large scale due to its high recovery rates and high adsorption capacity. © 2016 Society of Chemical Industry

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Mechanistic aspects of protein corona formation: insulin adsorption onto gold nanoparticle surfaces

Cited by 18 publications

References 90 publications

PVP-coated, negatively charged silver nanoparticles: A multi-center study of their physicochemical characteristics, cell culture and in vivo experiments

PVP-coated, negatively charged silver nanoparticles: A multi-center study of their physicochemical characteristics, cell culture and in vivo experiments

Cellular Response of Therapeutic Nanoparticles

Preparation of pH-responsive metal chelate affinity polymer for adsorption and desorption of insulin

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