Media for Enterobacteriaceae

Mossel, D. À. A.

doi:10.1016/0168-1605(85)90053-4

Cited by 25 publications

(7 citation statements)

References 23 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…The presence of Escherichia coli and Klebsiella spp. was determined using violet red bile glucose agar plates, which were incubated at 37 °C for 48 h [13]. Typical colonies were afterwards transferred to Levine agar and incubated at 45 °C for 24 h for E. coli isolation, and to MacConkey agar for Klebsiella spp.…”

Section: Methodsmentioning

confidence: 99%

Intravaginal Device-Type and Treatment-Length for Ovine Estrus Synchronization Modify Vaginal Mucus and Microbiota and Affect Fertility

Martínez-Ros

Lozano

Hernández³

et al. 2018

Animals

View full text Add to dashboard Cite

Simple SummaryThis study examined the effects of different intravaginal device types used for estrous cycle management in sheep, and the timing of their insertion, on vaginal features (characteristics of vaginal mucus discharge, pH and microbiota) and fertility under field conditions.AbstractInduction and synchronization of estrus and ovulation in sheep is based on intravaginal progestagen-impregnated polyurethane sponges or progesterone-loaded silicon-based devices (CIDR), in either short- (6–7 days) or long-term (12–14 days) protocols. Bearing in mind that the use of intravaginal sponges in long-term protocols has been related to the presence of vaginitis at removal, we compared the effects of sponges and CIDRs, maintained during either 7 or 14 days, on vaginal features (characteristics of vaginal mucus discharge, pH and microbiota) and fertility under field conditions. Almost all the ewes treated with intravaginal sponges showed vaginal discharge at device withdrawal, which was purulent and/or bloody in around 15% and 80% of the females treated for 7 and 14 days, respectively. The vaginal pH and microbiota changed in both groups when compared to control sheep, especially in ewes treated for 14 days, which showed a pH value around 8 and a higher incidence of Salmonella spp. and Staphylococcus aureus. On the other hand, independently of the length of the treatment, only around 15–20% of the sheep treated with CIDRs evidenced vaginal discharge (p < 0.00005 when compared to sponge groups), and such discharge was scarce, clear, and showed no changes in vaginal pH and microbiota when compared to control sheep. Fertility yields were associated with vaginal features, being higher in both short-term treatments (75%) and the long-term CIDR-based treatment (70%) than in the long-term sponge-based treatment (45%).

show abstract

Section: Methodsmentioning

confidence: 99%

Intravaginal Device-Type and Treatment-Length for Ovine Estrus Synchronization Modify Vaginal Mucus and Microbiota and Affect Fertility

Martínez-Ros

Lozano

Hernández³

et al. 2018

Animals

View full text Add to dashboard Cite

show abstract

“…The other half was aseptically diced, and a 50 g sample weighed out and blended with 450 mL 0.1 % peptone buffer @H 7.0) in a Waring Blendor for 2 min. Resulting slurries were serially diluted in peptone buffer, and duplicate samples plated on the following media: Tryptose Soy Agar (Difco) incubated at 30°C for 2 days and 5 "C for 10 days to give aerobic plate counts (APC) and psychrotroph counts, respectively; Violet Red Bile Agar (Difco) with 1% glucose (Mossel 1985) incubated at 32°C for 1 day to give Enterobacteriaceae (ENT) counts; acidified @H 3.5) Potato Dextrose Agar (PDA; Difco) incubated at 25 "C for 3 to 5 days to give yeast/mold counts; and de Man, Rogosa, Sharp-a agar + 0.1 % sorbic acid (Reuter 1985) incubated at 30°C for 2 days to give lactic acid bacteria counts. Plates to enumerate Enterobacteriaceae and all samples diluted 10-l were pour plated by depositing 1.0 mL of sample into the plate before addition of the melted media.…”

Section: Microbiological Analysismentioning

confidence: 99%

Changes in the Microflora of Packaged Fresh Tomatoes

Brackett

1988

Journal of Food Quality

View full text Add to dashboard Cite

Fresh tomatoes were shrink-wrapped in j l m , sealed in gas-flushed film pouches, or stored in cardboard packing crates. All samples were stored at 13 "C, and changes in populations of total aerobes, psychrotrophs, yeasts and molds, members of Enterobacteriaceae, and lactic acid bacteria followed. Shrink-wrapping and gas-packaging increased aerobic counts over no packaging, and selected for yeasts and molds. Shrink-wrapping additionally selected for lactic acid bacteria. 2he greatest number of psychrotrophs but the least number of yeasts and molds developed in unpackaged tomatoes. Shrink-wrapped and gas-packaged tomatoes remained unspoiled at least 6 weeks whereas controls spoiled in 4 weeks.

show abstract

“…Coliform bacterial counts were enumerated at 37°C for 18 h according to Mossel (1985) using violet red bile agar medium.…”

Section: Coliform Bacterial Countsmentioning

confidence: 99%

Manufacture of Functional Labneh Using Uf-Retentate and Artichoke Puree

Saad¹,

Salama²,

El‐Sayed³

2015

International J. of Dairy Science

View full text Add to dashboard Cite

Artichoke, UF-retentate and Bifidobacteria were used in success to manufacture novel functional labneh treatments in this study. Artichoke (Cynara scolymus L.) puree was added in ratios of 0, 15, 30 and 45% of the formula. Bifidobacterium bifidum, Lactobacillus bulgaricus and Streptococcus thermophilus (1:1) were added to produce lactic acid and healthy compounds. Total solids, fat/dry matter, ash, pH values, Total Nitrogen (TN), Soluble Nitrogen (SN) contents and Total Volatile Fatty Acids (TVFA) were determined. All samples texture profile measurements were also carried out. Streptococcus thermophilus continuously increased in all treatments followed by slight decrease towards the end of the storage period especially with control. Also, Bifidobacterium bifidium numerous increased during storage period to reach up to log 9.00 CFU gG 1 especially in the treatment that contained 30% artichoke puree. Results demonstrated that artichoke is a suitable food carrier that allows the survival of potentially probiotic strains. Preferences of treated samples 15 and 30% artichoke puree were as well as the control especially in outer appearance and flavour.

show abstract

Media for Enterobacteriaceae

Cited by 25 publications

References 23 publications

Intravaginal Device-Type and Treatment-Length for Ovine Estrus Synchronization Modify Vaginal Mucus and Microbiota and Affect Fertility

Intravaginal Device-Type and Treatment-Length for Ovine Estrus Synchronization Modify Vaginal Mucus and Microbiota and Affect Fertility

Changes in the Microflora of Packaged Fresh Tomatoes

Manufacture of Functional Labneh Using Uf-Retentate and Artichoke Puree

Contact Info

Product

Resources

About