Medicago sativa as a source of secondary metabolites for agriculture and pharmaceutical industry

Rafińska, Katarzyna; Pomastowski, Paweł; Wrona, Olga; Górecki, Ryszard J.

doi:10.1016/j.phytol.2016.12.006

Cited by 95 publications

(98 citation statements)

References 111 publications

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“…In accordance with some previous works, [31] the results have shown that the main phenolic compound in the extracts was ferulic acid which constituted approximately 5-15% of the prepared extracts. In addition to that, chromatograms of the extracts subjected to hydroliysis (Figure 4) shows that the glycosides of apigenin and kaempferol were present, as well as a small amount of p-coumaric acid.…”

Section: Total Phenols Flavonoids and Phenolic Acidssupporting

confidence: 92%

Multi-response Optimization of Ultrasound-assisted Extraction of Bioactive Components from Medicago Sativa L.

2017

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Response surface methodology was used to optimize experimental conditions for ultrasound-assisted extraction of functional components from Medicago sativa L. (Fabaceae) aerial parts. The procedure was performed according to the Box-Behnken design. The independent variables were ethanol concentration (X1), temperature (X2), and pH (X3), while the responses were extraction yield, DPPH radical scavenging activity of the extracts (RSA IC50), content of total phenols (TP), total flavonoids (TF) and total phenolic acids (TPA). The response values of the extracts prepared at optimum conditions (X1, X2, X3) were: yield (162 mg/ml, 20 %, 60 °C, 5.5), TP (6.63 mg/ml, 100 %, 60 °C, 5.5), TF (0.292 mg/ml, 100 %, 23 °C, 5.5), TPA (0.275 mg/ml, 100 %, 23 °C, 8.5) and RSA IC50 (3.97 mg/ml, 40 %, 60 °C, 7.0). The predicted values matched well with the observed ones indicating the suitability of the selected models for extraction of bioactive compounds from M. sativa.

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Section: Total Phenols Flavonoids and Phenolic Acidssupporting

confidence: 92%

Multi-response Optimization of Ultrasound-assisted Extraction of Bioactive Components from Medicago Sativa L.

2017

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“…They consist of sugar (glycon) and nonsugar parts (sapogenin) [1][2][3][4][5][6][7]. They consist of sugar (glycon) and nonsugar parts (sapogenin) [1][2][3][4][5][6][7].…”

Section: Introductionmentioning

confidence: 99%

“…They consist of sugar (glycon) and nonsugar parts (sapogenin) [1][2][3][4][5][6][7]. The properties of saponins are diverse and comprise fungicidal, antibacterial, cytotoxic, nematocidal, antiviral, and anticancer ones [1][2][3][4][5]12,13]. Holothuroidea, Asteroidea, and Porifera [5,[8][9][10][11][12][13].…”

Section: Introductionmentioning

confidence: 99%

“…Holothuroidea, Asteroidea, and Porifera [5,[8][9][10][11][12][13]. They are triterpenic pentacyclic glycosides with the following major sapogenins: medicagenic acid, hederagenin, bayogenin, and soyasapogenol B [3,7]. These compounds are used as preservatives, surfactants, Article Related Abbreviations: ASE, accelerated solvent extraction; ELSD, evaporative light scattering detection; SFE, supercritical fluid extraction.…”

Section: Introductionmentioning

confidence: 99%

“…The properties of saponins are diverse and comprise fungicidal, antibacterial, cytotoxic, nematocidal, antiviral, and anticancer ones [1][2][3][4][5]12,13]. Many studies have proven their effectiveness in combating insects (Leptinotarsa decemlineata, Lobesia botrana, Adoxophyes orana, Ostrinia nubilalis, the possibility of their application in agriculture against pests [3,17,19]. emulsifiers, or bronchodilators [11,14].…”

Section: Introductionmentioning

confidence: 99%

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Distribution of sapogenins in morphological Medicago sativa L. parts: Comparison of various extraction techniques

Kiełbasa

Krakowska-Sieprawska

Kowalkowski

et al. 2019

J of Separation Science

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Saponins in plant extracts were indirectly determined by estimation of the content of sapogenins. The first step of determination is extraction with high efficiency. One conventional extraction technique (maceration) and two modern ones (accelerated solvent extraction and supercritical fluid extraction) were compared. Methanol and ethanol were used as solvents or co-solvents. The results were supported by statistical analysis. Saponins were extracted from leaves, roots, and sprouts of Medicago sativa. Acid hydrolysis, purification, and determination by high-performance liquid chromatography with evaporative light scattering detector were used. The content of sapogenins was the highest in the roots. Smaller amounts of sapogenins were found in sprouts and the smallest ones in leaves. The main ingredient was medicagenic acid with mean concentration of 621.8 μg/g in roots, 456.7 μg/g in sprouts, and 471.3 μg/g in leaf extract. The highest content of sapogenins in extract was obtained after maceration with methanol; however, this method is nonselective in relation to biologically active compounds. Due to the possibility of using the obtained extracts with sapogenins in the cosmetic or pharmaceutical industry, the selection of extraction techniques and solvents is a very important aspect. Additionally, the chosen technique should be considered eco-friendly and consistent with the assumptions of "green chemistry." K E Y W O R D Sextraction methods, liquid chromatography, Medigaco sativa, sapogenins, statistical analysis Tenebrio molitor, and Spodoptera littoralis) and nematodes (Meloidogyne incognita, Globodera rostochiensis, and Xiphinema index) [15][16][17][18]. Therefore, plant materials especially rich in saponins are searched for because of J Sep Sci 2020;43:671-680.

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Do plant secondary metabolite‐containing forages influence soil processes in pasture systems?

et al. 2020

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Grazed pastures are susceptible to N loss from urine/manure additions, which increases eutrophication, affecting the global N cycle. Plant secondary metabolites (PSM), such as condensed tannins (CT) and terpenes, influence silviculture soil dynamics by generally decreasing N mineralization. We investigated whether cattle-grazed pastures of non-traditional grass and legume forage monoculture strips including CT-containing sainfoin (Onobrychis viciifolia Scop.) and tall fescue (TF) [Schedonorus arundinaceus (Schreb.) Dumort.] influenced soil dynamics compared with traditional grass and legume forage monoculture strips of alfalfa (Medicago sativa L.), without tannins, and TF. Throughout the study, CT in sainfoin averaged 58.9 g kg −1 whereas alfalfa saponins averaged 5.7 g kg −1. We observed greater soil microbial respiration (p = .01) in TF strips than legume strips, indicating greater microbial activity, and between legumes we found greater soil NO 3 (p = .01) in alfalfa than in sainfoin, although aboveground biomass and N differences were negligible. We also conducted a laboratory soil-feces incubation study to determine if feces from cattle foraging diets of legumes with or without CT influenced soil dynamics. Both feces treatments showed lower NO 3 (p < .001) than without feces, suggesting microbial inhibition. Dehydrogenase activity (DHEA) was lower (p = .03) in sainfoin than alfalfa feces, suggesting CT from sainfoin inhibit DHEA. To our knowledge this study is the first considering whether CT-containing sainfoin and saponin-containing alfalfa influence soil dynamics by assessing general differences in soil parameters. More research is needed to determine whether specific PSM mitigate N loss in pasture systems by slowing N mineralization.

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Medicago sativa as a source of secondary metabolites for agriculture and pharmaceutical industry

Cited by 95 publications

References 111 publications

Multi-response Optimization of Ultrasound-assisted Extraction of Bioactive Components from Medicago Sativa L.

Multi-response Optimization of Ultrasound-assisted Extraction of Bioactive Components from Medicago Sativa L.

Distribution of sapogenins in morphological Medicago sativa L. parts: Comparison of various extraction techniques

Do plant secondary metabolite‐containing forages influence soil processes in pasture systems?

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