Medicago truncatula resources to study legume biology and symbiotic nitrogen fixation

Nandety, Raja Sekhar; Wen, Jiangqi; Mysore, Kirankumar S.

doi:10.1016/j.fmre.2022.06.018

Cited by 4 publications

(4 citation statements)

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“…Mapping of the hairy vetch contigs against M. truncatula genome (V4.0) resulted in the identification of 76,422 predicted genes in hairy vetch. The rational for the use of M. truncatula genome is that it is a model legume species, and it's genome, genes, and transcriptome data are well characterized, well curated, and updated (Carrere et al., 2021; Garmier et al., 2017; He et al., 2009; Nandety et al., 2022).…”

Section: Resultsmentioning

confidence: 99%

Transcript profiling of hairy vetch (Vicia villosaRoth) identified interesting genes for seed dormancy

et al. 2023

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Hairy vetch, a diploid annual legume species, has a robust growth habit, high biomass yield, and winter hardy characteristics. Seed hardness is a major constraint for growing hairy vetch commercially. Hard seeded cultivars are valuable as forages, whereas soft seeded and shatter resistant cultivars have advantages for their use as a cover crop. Transcript analysis of hairy vetch was performed to understand the genetic mechanisms associated with important hairy vetch traits. RNA was extracted from leaves, flowers, immature pods, seed coats, and cotyledons of contrasting soft and hard seeded “AU Merit” plants. A range of 31.22–79.18 Gb RNA sequence data per tissue sample were generated with estimated coverage of 1040–2639×. RNA sequence assembly and mapping of the contigs against the Medicago truncatula (V4.0) genome identified 76,422 gene transcripts. A total of 24,254 transcripts were constitutively expressed in hairy vetch tissues. Key genes, such as KNOX4 (a class II KNOTTED‐like homeobox KNOXII gene), qHs1 (endo‐1,4‐β‐glucanase), GmHs1‐1 (calcineurin‐like metallophosphoesterase), chitinase, shatterproof 1 and 2 (SHP1, SHP2), shatter resistant 1–5 (SHAT1–5)(NAC transcription factor), PDH1 (prephenate dehydrogenase 1), and pectin methylesterases with a potential role in seed hardness and pod shattering, were further explored based on genes involved in seed hardness from other species to query the hairy vetch transcriptome data. Identification of interesting candidate genes in hairy vetch can facilitate the development of improved cultivars with desirable seed characteristics for use as a forage and as a cover crop.

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Section: Resultsmentioning

confidence: 99%

Transcript profiling of hairy vetch (Vicia villosaRoth) identified interesting genes for seed dormancy

et al. 2023

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“…Alfalfa ( Medicago sativa ) is a leguminous plant widely cultivated around the world, which is used mainly as fodder. From researchers’ point of view, alfalfa is considered a crop model plant because Medicago truncatula , another species of the same genus, is a model legume whose genome is fully sequenced and many other genetic tools (mutants, small secreted peptides (SSPs) database, transporter protein database, gene expression atlas, proteomic atlas, and metabolite atlas) are available [ 78 ]. The results indicate that this plant is very sensitive to metal pollution because growth and physiology were significantly affected [ 79 , 80 ].…”

Section: Discussionmentioning

confidence: 99%

A Culturomics-Based Bacterial Synthetic Community for Improving Resilience towards Arsenic and Heavy Metals in the Nutraceutical Plant Mesembryanthemum crystallinum

Flores-Duarte

Pajuelo

Mateos‐Naranjo

et al. 2023

IJMS

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Plant-growth-promoting bacteria (PGPB) help plants thrive in polluted environments and increase crops yield using fewer inputs. Therefore, the design of tailored biofertilizers is of the utmost importance. The purpose of this work was to test two different bacterial synthetic communities (SynComs) from the microbiome of Mesembryanthemum crystallinum, a moderate halophyte with cosmetic, pharmaceutical, and nutraceutical applications. The SynComs were composed of specific metal-resistant plant-growth-promoting rhizobacteria and endophytes. In addition, the possibility of modulating the accumulation of nutraceutical substances by the synergetic effect of metal stress and inoculation with selected bacteria was tested. One of the SynComs was isolated on standard tryptone soy agar (TSA), whereas the other was isolated following a culturomics approach. For that, a culture medium based on M. crystallinum biomass, called Mesem Agar (MA), was elaborated. Bacteria of three compartments (rhizosphere soil, root endophytes, and shoot endophytes) were isolated on standard TSA and MA media, stablishing two independent collections. All bacteria were tested for PGP properties, secreted enzymatic activities, and resistance towards As, Cd, Cu, and Zn. The three best bacteria from each collection were selected in order to produce two different consortiums (denominated TSA- and MA-SynComs, respectively), whose effect on plant growth and physiology, metal accumulation, and metabolomics was evaluated. Both SynComs, particularly MA, improved plant growth and physiological parameters under stress by a mixture of As, Cd, Cu, and Zn. Regarding metal accumulation, the concentrations of all metals/metalloids in plant tissues were below the threshold for plant metal toxicity, indicating that this plant is able to thrive in polluted soils when assisted by metal/metalloid-resistant SynComs and could be safely used for pharmaceutical purposes. Initial metabolomics analyses depict changes in plant metabolome upon exposure to metal stress and inoculation, suggesting the possibility of modulating the concentration of high-value metabolites. In addition, the usefulness of both SynComs was tested in a crop plant, namely Medicago sativa (alfalfa). The results demonstrate the effectiveness of these biofertilizers in alfalfa, improving plant growth, physiology, and metal accumulation.

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“…Genome Wide Association Studies (GWAS) is a newer experimental approach that uses natural genomic variations correlated with observed phenotypic variations in a population, using statistical methods to identify novel determinants underlying a trait (Uffelmann et al, 2021). Resources in M. truncatula that make it a model plant to study root nodule symbiosis include a robust sequenced population of Haplotype mapping or ‘HapMap’ lines with sufficient genomic sequence variation to conduct GWA studies (Nandety et al, 2023). GWAS using the Medicago HapMap lines have helped uncover genomic loci that influence the phenotype of interest such as climatic adaptation, salt stress, seed size and composition, disease resistance and, ability to form nodules (Rey et al, 2017; Guerrero et al, 2018; Kang et al, 2019; Bonhomme et al, 2021; Chen et al, 2021a; Chen et al, 2021b).…”

Section: Introductionmentioning

confidence: 99%

A Multitrait Genome-Wide Association Study Reveals a Requirement for the Strigolactone Receptor MtDWARF14 in Optimal GOLVEN Signaling

Roy,

Kang,

Zhang

et al. 2024

Preprint

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GOLVEN/ROOT MERISTEM GROWTH FACTOR family of signaling peptides have been shown to control root lateral organ number, density and positioning in plants, although the signaling pathways involved remain obscure. A diverse set of 171Medicago truncatulaHapMap accessions with variation in responses to the GOLVEN 10 peptide, GLV10, were used to identify 74 significant loci controlling seven traits related to nodule formation and root architecture. Importantly, a single nucleotide polymorphism (SNP) in the upstream region of the MtGLV10 peptide-inducible strigolactone receptor gene,MtDWARF14was significantly associated with insensitivity of nodule density to GLV10, suggesting a link between strigolactone signaling and GLV10 responsiveness. Three independentd14mutants of the DWARF14gene were found to hypernodulate, while overexpression of the gene led to reduction in nodule number, phenocopying GLV10. A null mutant,mtd14-1, remained sensitive to GLV10’s effect on nodule density. However, at the transcriptional level, the mutant failed to effectively induce the expression of the GOLVEN marker genes,MtPLETHORA3andMtPINLIKES2. Our study uncovers a hitherto unknown link between the strigolactone and GLV peptide signaling pathways using genotype x environment analysis of Medicago HapMap lines and provides a putative molecular mechanism for recovery from frost damage to fine roots.

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Medicago truncatula resources to study legume biology and symbiotic nitrogen fixation

Cited by 4 publications

References 95 publications

Transcript profiling of hairy vetch (Vicia villosaRoth) identified interesting genes for seed dormancy

Transcript profiling of hairy vetch (Vicia villosaRoth) identified interesting genes for seed dormancy

A Culturomics-Based Bacterial Synthetic Community for Improving Resilience towards Arsenic and Heavy Metals in the Nutraceutical Plant Mesembryanthemum crystallinum

A Multitrait Genome-Wide Association Study Reveals a Requirement for the Strigolactone Receptor MtDWARF14 in Optimal GOLVEN Signaling

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