To God, for giving me strength, to illuminate my ways and to allow me to achieve this goal.To my family, for my education, support, affection, and dedication, and for being always by my side, helping me.To the adviser Silvio Hamacher, for the knowledge, guides, lessons, and motivations. He is one of the most notable examples I've had in my life. I am eternally grateful for all the doors that he opened for me, his continuous support, and trust.It was an honor for me having the opportunity to work with Prof. Kalyan S. Pasupathy and Prof. Mustafa Y. Sir at Mayo Clinic. It was an incredible experience to have the chance to work with them and his group. My sincere thanks go to them for the support and dedication to me during my stay.To Leila, Igor, Victor, and Leonardo, for their partnership in studies and moments of relaxation that has become a friendship that I want to take forever.To Stephanie, for patience and unconditional support, believing and making me believe in my potential during my PhD.To Luana, Iuri, Pedro, João Gabriel, Luiza, Gabriela, Danuza, and other colleagues, for their receptivity and collaboration in the work environment.To the many colleagues and professors of PUC-Rio, for providing me with subsidies that allowed me to obtain a valuable academic improvement.To CNPq, FAPERJ, Tecgraf, and PUC-Rio, for the aid granted and the excellent study environment, without which this work would not have been possible.I want to thank to my special friends (Giovanna, Romerito, Jânia, Juliane, Bruna, my EJC's friends, my friends from PSM, and many others) and all those people that were around me providing emotional support and comfort.To all those who, in some way, contributed to this work. This study was financed in part by the Coordenação de Aperfeiçoamento de Pessoal de Nível Superior -Brasil (CAPES) -Finace Code 001.