Medicinal plants extracts affect virulence factors expression and biofilm formation by the uropathogenic Escherichia coli

Wojnicz, Dorota; Kucharska, Alicja Z.; Sokół‐Łętowska, Anna; Kicia, Marta; Tichaczek−Goska, Dorota

doi:10.1007/s00240-012-0499-6

Cited by 103 publications

(64 citation statements)

References 34 publications

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“…The loss of congo red-binding abilities by the tested rods was observed only at concentrations of 40 and 50 µg/mL of both acids used. We obtained similar results in our previous research (Wojnicz et al, 2012). Results of the current study indicate that AA and UA have relatively similar inhibitory activities on curli fiber synthesis, although it should be noted that AA caused the , 11, 824 5 060, 11, 824, 529, 964 5 060, 11, 824, 529, 964 6 060, 11, 824, 529, 964, 590 6 060, 11, 824, 529, 964, 590 30, 399 4 11, 30, 399, 618 Alpha-hemolysin (n = 11) 0 ---0 - --1 192 2 192, 060 7 192,060,14,419,448,679,964 -4 14, 419, 448, 679 8 14, 419, 448, 679, 060, 192, 590, 964 loss of curli fibers from cell surfaces of 7 strains, while UA caused loss in only 4 cases.…”

Section: Curli Fiber Expressionsupporting

confidence: 92%

“…The presence of curli fibers coded by this gene allows bacteria to bind to the host cell membrane proteins, colonize the renal tubular epithelium more efficiently, and produce biofilm. The prevalence of the papC gene among clinical isolates collected by us corresponds to results reported earlier (Borisova et al, 2004;Wojnicz et al, 2012). However, the distribution of the hlyA gene found in the current study was higher than reported by Arisoy et al (2008) and Farshad et al (2009).…”

Section: Molecular Characterization Of Bacterial Strainsupporting

confidence: 89%

“…Sequence coding for 16S rRNA was used as a positive control. The characteristics of all used primers, except draE primers, as well as amplicon lengths, were listed in our previous study (Wojnicz et al, 2012). Primers 5'-GACAGTTTACTGATTCTGGGA-3' and 5'-ACGTCCAATAATGCTTACCC-3' were used in the PCR analysis to amplify the draE gene (Matar et al, 2005).…”

Section: Phylogenetic Classification and Virulence-associated Gene Camentioning

confidence: 99%

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Effect of asiatic and ursolic acids on growth and virulence factors of uropathogenic Escherichia coli strains

Wojnicz¹,

Tichaczek−Goska²,

Kicia³

2013

Turk J Biol

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This work was aimed at determining the possible antimicrobial potential of asiatic acid (AA) and ursolic acid (UA) against clinical uropathogenic Escherichia coli strains (UPECs). Phylogenetic affiliations of UPECs and virulence-associated gene carriage have been identified. PCR analysis revealed that the studied E. coli strains belong to phylogenetic group B2. All bacterial isolates were grouped into 4 pathotypes. The following were determined: the minimum inhibitory concentrations (MICs) according to CLSI; P-fimbriae synthesis, based on erythrocyte agglutination; curli fibers, by using YESCA agar with congo red; swimming motility, in motility agar; and alpha-hemolysin, on sheep's-blood agar plates. The differences in antigrowth activities of AA and UA were recorded. Inhibition of P-fimbriae, curli fibers, and alpha-hemolysin production after exposure of UPECs to both AA and UA were observed. Reduction of bacterial motility was also noticed. Our results clearly show the influence of AA and UA on virulence factors of UPEC strains. However, differences between antivirulence activities of AA and UA were not found, although a higher level of antigrowth activity of AA in comparison with UA was shown.

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Section: Curli Fiber Expressionsupporting

confidence: 92%

Section: Molecular Characterization Of Bacterial Strainsupporting

confidence: 89%

Section: Phylogenetic Classification and Virulence-associated Gene Camentioning

confidence: 99%

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Effect of asiatic and ursolic acids on growth and virulence factors of uropathogenic Escherichia coli strains

Wojnicz¹,

Tichaczek−Goska²,

Kicia³

2013

Turk J Biol

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“…This therapy involves the combination of photosensitizer (PS), light and molecular oxygen (de Melo et al, 2013). In addition, strategies involving nanoparticles (Allaker, 2010; Ramasamy et al, 2016), plant extracts (Wojnicz et al, 2012), and chitosan (Carlson et al, 2008) have been applied against microbial biofilm, with significant results. Further studies are necessary for identifying means to overcome fungal biofilm resistance.…”

Section: Biofilm and Antifungal Resistancementioning

confidence: 99%

Antifungal Therapy: New Advances in the Understanding and Treatment of Mycosis

et al. 2017

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The high rates of morbidity and mortality caused by fungal infections are associated with the current limited antifungal arsenal and the high toxicity of the compounds. Additionally, identifying novel drug targets is challenging because there are many similarities between fungal and human cells. The most common antifungal targets include fungal RNA synthesis and cell wall and membrane components, though new antifungal targets are being investigated. Nonetheless, fungi have developed resistance mechanisms, such as overexpression of eﬄux pump proteins and biofilm formation, emphasizing the importance of understanding these mechanisms. To address these problems, different approaches to preventing and treating fungal diseases are described in this review, with a focus on the resistance mechanisms of fungi, with the goal of developing efficient strategies to overcoming and preventing resistance as well as new advances in antifungal therapy. Due to the limited antifungal arsenal, researchers have sought to improve treatment via different approaches, and the synergistic effect obtained by the combination of antifungals contributes to reducing toxicity and could be an alternative for treatment. Another important issue is the development of new formulations for antifungal agents, and interest in nanoparticles as new types of carriers of antifungal drugs has increased. In addition, modifications to the chemical structures of traditional antifungals have improved their activity and pharmacokinetic parameters. Moreover, a different approach to preventing and treating fungal diseases is immunotherapy, which involves different mechanisms, such as vaccines, activation of the immune response and inducing the production of host antimicrobial molecules. Finally, the use of a mini-host has been encouraging for in vivo testing because these animal models demonstrate a good correlation with the mammalian model; they also increase the speediness of as well as facilitate the preliminary testing of new antifungal agents. In general, many years are required from discovery of a new antifungal to clinical use. However, the development of new antifungal strategies will reduce the therapeutic time and/or increase the quality of life of patients.

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“…Rosemary water extracts (RWE1 and RWE2) were prepared from the fresh rosemary leafs collected from Taif and Cairo, respectively, according to the method of Wojnicz et al . (), with some modifications. Rosemary leaves were dried at 37 °C for 48 h (to reach about 9.6% moisture content; dry basis).…”

Section: Methodsmentioning

confidence: 97%

Antibiofilm formation and anti‐adhesive (to HEp‐2 cells) effects of rosemary water extract against some food‐related pathogens

Elhariry

Abuzaid

Khiralla

et al. 2013

Int J of Food Sci Tech

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Summary The present work aimed to determine the bioactive compounds in two rosemary water extracts (RWE1 and RWE2) and to assess their antimicrobial, anti‐adhesive and antibiofilm potentials against the food‐related Bacillus and Pseudomonas species at concentrations; 4, 8, 12, 16 and 20 mg mL−1. Phenolic compounds and isoflavones in the RWEs were determined using HPLC. The concentrations of most bioactive compounds of RWE1 (benzoic, ellagic, gallic and rosmarinic acids, daidzein and genistein) were higher than that of RWE2. The MIC90 of RWE1 and RWE2 against all tested bacteria was 12 and 16 mg mL−1, respectively. The anti‐adhesive and antibiofilm doses were higher than MIC90. RWE1 and RWE2 showed potential reduction in the bacterial cell adhesion to HEp‐2 cells – 17.5–64.7 and 12.2–52.9%, respectively. In conclusion, this study emphasises the effective use of RWE as a natural anti‐adhesive and antibiofilm agent against Bacillus and Pseudomonas, without difficult extraction procedure.

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Medicinal plants extracts affect virulence factors expression and biofilm formation by the uropathogenic Escherichia coli

Cited by 103 publications

References 34 publications

Effect of asiatic and ursolic acids on growth and virulence factors of uropathogenic Escherichia coli strains

Effect of asiatic and ursolic acids on growth and virulence factors of uropathogenic Escherichia coli strains

Antifungal Therapy: New Advances in the Understanding and Treatment of Mycosis

Antibiofilm formation and anti‐adhesive (to HEp‐2 cells) effects of rosemary water extract against some food‐related pathogens

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