Medium composition, coconut water, and chitosan effects on <i>Phalaenopsis</i> protocorm growth and development

Sukma, Dewi; Aziz, Sandra Arifin; Andini, Nur; Putri, Halida Adistya; Sudarsono, Sudarsono

doi:10.17660/actahortic.2019.1262.18

Cited by 3 publications

(1 citation statement)

References 13 publications

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“…Similar results were recorded by Anusuya and Sathiyabama [27], who revealed that growth parameters (shoot height, leaf number/plant, plant fresh weight) increased with application of chitosan. However, in contrast, Sukma et al [28] suggested that chitosan had a negative effect on Phalaenopsis protocorm growth by decreasing protocorm multiplication and plantlet regeneration. Optimal orchid growth at various chitosan concentrations depends on several factors, including plant species, explants, and developmental stage.…”

Section: Chitosan Treatmentmentioning

confidence: 94%

Effect of Chitosan, Coconut Water and Potato Extract on Protocorm Growth and Plantlet Regeneration of Cymbidium aloifolium (L.) Sw.

Taratima

Rohmah

2021

Curr. Appl. Sci. Technol.

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Effective and rapid in vitro plantlet regeneration from protocorms of Cymbidium aloifolium using chitosan, coconut water and potato extract were investigated. Two experiments were performed using twelve week old protocorms of size 2-3 mm. In the first experiment, the protocorms were propagated on solid New Dogashima (ND) medium supplemented with various concentrations of chitosan (0, 0.05, 0.10 and 1.00 mg/l). For the second experiment, the protocorms were cultured on the same medium supplemented with coconut water (0, 100, 150 and 200 ml/l) and potato extract (0, 5, 10 and 15 g/l). Survival and response percentages, shoot number and length, root number and length, leaf number and fresh and dry weight were collected after 10 weeks of culture. The results showed that 0.05 mg/l chitosan treatment was the most effective for protocorm growth, producing the highest shoot number and length, root number and length, leaf number, and fresh and dry weight. The combination of coconut water and potato extract treatments revealed that 150 ml/l coconut water alone was the best for plantlet regeneration and growth, shoot length, root length, and fresh and dry weight. This treatment gave significantly different results from the other treatments. Findings will provide useful information for other in vitro commercial or conservation orchid propagation programs.

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Section: Chitosan Treatmentmentioning

confidence: 94%

Effect of Chitosan, Coconut Water and Potato Extract on Protocorm Growth and Plantlet Regeneration of Cymbidium aloifolium (L.) Sw.

Taratima

Rohmah

2021

Curr. Appl. Sci. Technol.

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Micropropagation of Trichopilia suavis Lindl. & Paxton

Hussien,

Molkanova,

Mitrofanova

2023

Ornam. Hortic.

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Trichopilia suavis is one of the important representatives of the genus Trichopilia, which can be used as a potted plant for the interior design. also, its beautiful inflorescences are added unusual element to bouquet compositions and actively used in the production of perfumes. This study was carried out in order to develop and improve micropropagation method for mass clonal production of T. suavis through protocorm culture. Half-strength Murashige and Skoog culture medium (½) has been modified by adding different organic components and growth regulators. The protocorms obtained from seeds germinated by asymbiotic method were used as explants. The results showed that the optimal treatment for formation of new protocorms (7.50 ± 0.7 units per protocorm) was obtained by culture on ½ MS medium with 0.5 mg L-1 of 6-Benzylaminopurine (6-BAP) and 100 mL L-1 of coconut water. At the stage of rooting, the highest number and length of roots (7.0 ± 0.45 units per plantlet, 2.64 ± 0.18 cm) were obtained through subsequent cultivation of plantlets on ½ MS medium with 0.5 mg L-1 indolyl-3-butyric acid (IBA), 1.0 g L-1 charcoal with the addition of 50 g L-1 of banana puree. The obtained seedlings were successfully adapted in a substrate consisting of bark, perlite and peat in a ratio of 1:1:1.

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Optimization of Rice (Oryza sativa L.) ‘Tubtim Chumphae', for Callus Induction, Proliferation and Plantlet Regeneration

Taratima¹,

Plaikhunto²,

Wichachai³

et al. 2022

Asian J. of Plant Sciences

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Medium composition, coconut water, and chitosan effects on Phalaenopsis protocorm growth and development

Cited by 3 publications

References 13 publications

Effect of Chitosan, Coconut Water and Potato Extract on Protocorm Growth and Plantlet Regeneration of Cymbidium aloifolium (L.) Sw.

Effect of Chitosan, Coconut Water and Potato Extract on Protocorm Growth and Plantlet Regeneration of Cymbidium aloifolium (L.) Sw.

Micropropagation of Trichopilia suavis Lindl. & Paxton

Optimization of Rice (Oryza sativa L.) ‘Tubtim Chumphae', for Callus Induction, Proliferation and Plantlet Regeneration

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