Medium design from corncob hydrolyzate for pigment production by Talaromyces atroroseus GH2: Kinetics modeling and pigments characterization

Morales‐Oyervides, Lourdes; Ruiz‐Sánchez, Juan Pablo; Oliveira, Jorge C.; Sousa‐Gallagher, Maria J.; Morales-Martínez, Thelma K.; Albergamo, Ambrogina; Salvo, Andrea; Giuffrida, Daniele; Dufossé, Laurent; Montañez, Julio

doi:10.1016/j.bej.2020.107698

Cited by 25 publications

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“…Indeed, it is well known that if a fungus has favorable nutrient concentrations and appropriate C/N ratio, it will mainly produce biomass representing the fungal growth. However, when an imbalance appears, it will produce pigments expressing as a stress factor for the fungus [ 106 , 107 , 108 ]. Thus, carbon and nitrogen concentrations as well as C:N ratios have significant impact, which is proven to be strain dependent [ 109 ].…”

Section: Discussionmentioning

confidence: 99%

OVAT Analysis and Response Surface Methodology Based on Nutrient Sources for Optimization of Pigment Production in the Marine-Derived Fungus Talaromyces albobiverticillius 30548 Submerged Fermentation

et al. 2021

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Pigment production from filamentous fungi is gaining interest due to the diversity of fungal species, the variety of compounds synthesized, and the possibility of controlled massive productions. The Talaromyces species produce a large panel of metabolites, including Monascus-like azaphilone pigments, with potential use as natural colorants in industrial applications. Optimizing pigment production from fungal strains grown on different carbon and nitrogen sources, using statistical methods, is widespread nowadays. The present work is the first in an attempt to optimize pigments production in a culture of the marine-derived T. albobiverticillius 30548, under the influence of several nutrients sources. Nutrient combinations were screened through the one-variable-at-a-time (OVAT) analysis. Sucrose combined with yeast extract provided a maximum yield of orange pigments (OPY) and red pigments (RPY) (respectively, 1.39 g/L quinizarin equivalent and 2.44 g/L Red Yeast pigment equivalent), as well as higher dry biomass (DBW) (6.60 g/L). Significant medium components (yeast extract, K2HPO4 and MgSO4·7H2O) were also identified from one-variable-at-a-time (OVAT) analysis for pigment and biomass production. A five-level central composite design (CCD) and a response surface methodology (RSM) were applied to evaluate the optimal concentrations and interactive effects between selected nutrients. The experimental results were well fitted with the chosen statistical model. The predicted maximum response for OPY (1.43 g/L), RPY (2.59 g/L), and DBW (15.98 g/L) were obtained at 3 g/L yeast extract, 1 g/L K2HPO4, and 0.2 g/L MgSO4·7H2O. Such optimization is of great significance for the selection of key nutrients and their concentrations in order to increase the pigment production at a pilot or industrial scale.

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Section: Discussionmentioning

confidence: 99%

OVAT Analysis and Response Surface Methodology Based on Nutrient Sources for Optimization of Pigment Production in the Marine-Derived Fungus Talaromyces albobiverticillius 30548 Submerged Fermentation

et al. 2021

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“…The production of azaphilones increases as a survival mechanism directed to the neutralization of fungicide effects rather than a decrease in antifungal concentration [101]. Cost minimization for industrial production of azaphilones can be reduced by using agro-industrial waste as material for fungal growth, which also helps to solve the problem of pollution associated with the disposal of residues in the environment [19,98]. Liu et al Cost minimization for industrial production of azaphilones can be reduced by using agro-industrial waste as material for fungal growth, which also helps to solve the problem of pollution associated with the disposal of residues in the environment [19,98].…”

Section: Yield Improvementmentioning

confidence: 99%

“…Cost minimization for industrial production of azaphilones can be reduced by using agro-industrial waste as material for fungal growth, which also helps to solve the problem of pollution associated with the disposal of residues in the environment [19,98]. Liu et al Cost minimization for industrial production of azaphilones can be reduced by using agro-industrial waste as material for fungal growth, which also helps to solve the problem of pollution associated with the disposal of residues in the environment [19,98]. Liu et al [102] used rice straw hydrolysate for pigment production by M. purpureus M630 but reported that this substrate and does not have the ideal carbon content required by the fungus.…”

Section: Yield Improvementmentioning

confidence: 99%

“…Pigments production by T. atroroseus strain GH2 was studied in two different culture media (pH 5.0, 30 ± 2 °C, 200 rpm, 8 days) [ 98 ]. The first one was composed by synthetic Czapek-dox modified medium containing high levels of xylose, with and without nutrients supplementation and the second medium was composed by hydrolyzed corncob, a lignocellulosic waste.…”

Section: Processing and Innovations In Azaphilones Productionmentioning

confidence: 99%

“…T. atroroseus GH2 demonstrated a significantly different response to the carbon and nitrogen composition of the culture media, with improved growth and enhanced pigments production in the hydrolyzed corncob medium without any nutrient supplementation. Therefore, T. atroroseus was pointed by the authors as a promising pigment-producing microorganism for economically competitive large-scale fermentation at lower cost [ 98 ].…”

Section: Processing and Innovations In Azaphilones Productionmentioning

confidence: 99%

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Recent Findings in Azaphilone Pigments

Pimenta

Gomes

Cardoso

et al. 2021

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Filamentous fungi are known to biosynthesize an extraordinary range of azaphilones pigments with structural diversity and advantages over vegetal-derived colored natural products such agile and simple cultivation in the lab, acceptance of low-cost substrates, speed yield improvement, and ease of downstream processing. Modern genetic engineering allows industrial production, providing pigments with higher thermostability, water-solubility, and promising bioactivities combined with ecological functions. This review, covering the literature from 2020 onwards, focuses on the state-of-the-art of azaphilone dyes, the global market scenario, new compounds isolated in the period with respective biological activities, and biosynthetic pathways. Furthermore, we discussed the innovations of azaphilone cultivation and extraction techniques, as well as in yield improvement and scale-up. Potential applications in the food, cosmetic, pharmaceutical, and textile industries were also explored.

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