Medium Effect on Heterologous Protein Leaky Expression in
            <i>Escherichia coli</i>

Mirzahoseini, Hasan; Shahbazzadeh, Delavar; Enayati, Somayeh; Razzaghi-Abyaneh, Mehdi; Mahboudi, F

doi:10.1002/9783527611904.ch115

Cited by 2 publications

(2 citation statements)

References 5 publications

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“…The expression of recombinant products is an essential method for producing target proteins and one of the most important unit processes for production of therapeutic protein and structural study [1][2][3][4][5][6]. There are different expression systems for producing recombinant protein in biotechnology industry.…”

Section: Introductionmentioning

confidence: 99%

Retracted: Chemical Assistance in Refolding of Bacterial Inclusion Bodies

Alibolandi¹,

Mirzahosei²

2011

American J. of Biochemistry and Molecular Biology

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Escherichia coli is one of the most widely used hosts for the production of recombinant proteins but insoluble expression of heterologous proteins is a major bottleneck in production of recombinant proteins in E. coli. In vitro refolding of inclusion body into proteins with native conformations is a solution for this problem but there is a need for optimization of condition for each protein specifically. Several approaches have been described for in vitro refolding; most of them involve the use of additives for assisting correct folding. Cosolutes play a major role in refolding process and can be classified according to their function as aggregation suppressors and folding enhancers. This paper presents a review of additives that are used in refolding process of insoluble recombinant proteins in small scale and industrial processes.

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Section: Introductionmentioning

confidence: 99%

Retracted: Chemical Assistance in Refolding of Bacterial Inclusion Bodies

Alibolandi¹,

Mirzahosei²

2011

American J. of Biochemistry and Molecular Biology

View full text Add to dashboard Cite

show abstract

“…The expression of recombinant products is an essential method for producing target proteins and one of the most important unit processes for production of therapeutic protein and structural study [ 1 – 6 ]. There are different expression systems for producing recombinant protein in biotechnology industry.…”

Section: Introductionmentioning

confidence: 99%