Medium effects on micropropagation and genetic stability of &amp;lt;i&amp;gt;Citrullus lanatus&amp;lt;/i&amp;gt; oleaginous type

Gnamien, Yah Gwladys; Bi, Irié Arsène Zoro; Kouadio, Yatty Justin; Brostaux, Yves; Baudoin, Jean-Pierre

doi:10.4236/as.2013.47a005

Cited by 2 publications

(2 citation statements)

References 46 publications

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“…On the other contarary, Gnamien et al (2013) tested different concentrations of MS mineral elements and BAP to regenerate adventitious shoots from the cotyledons of Citrullus lanatus. They found that the medium containing 3/2 strength of MS mineral elements and 1 mg/l BAP allowed the production of numerous shoots.…”

Section: Means With the Same Letter In The Same Column Are Not Signifmentioning

confidence: 99%

Tissue Culture Studies on Propagation of Some Ornamental Plants 1. Starting and Shooting Stages of Phytolacca dioica L.

El-Afry

Nofal

Saadawy³

et al. 2017

Journal of Plant Production

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This work was carried out in the Tissue Culture Egypt from the period elapsed from 2014 to 2016 to study the effect of sterilization on propagation of Phytolacca dioica, L.. Results of this study could be briefed in the following outlines: Chlorox concentrations at 30 or 35 % gave the highest survival percentage of Phytolacca dioica, L.. In addition, soaking explants in the sterilant solution for 20 or 25 minutes resulted in the highest survival percentage. Similarly, the interaction treatment demonstrated that using chlorox at 35% for 25 minutes resulted in the highest survival%. In the multiplication stage it was found that using BAP at 10 ppm resulted in the highest number of shoots and leaves. BAP at 6 or 8 ppm induced the highest content of total chlorophyll. The longest shoots were a result of applying BAP at 3 ppm. Inoculating explants on half strength medium resulted in the highest values of number of shoots and leaves, as well as highest contents of total chlorophyll and carotenoids. MS medium at 3 quarters strength shared in the first position of number of leaves. The longest shoots were a result of using MS medium at full strength. The interaction between BAP levels and MS medium strength, indicated that using MS medium at half strength supplemented with BAP at 10 ppm produced highest number of shoots. The same medium strength with BAP at 8 or 6 ppm gave the highest contents of total chlorophyll and carotenoids. MS medium at 3 quarters strength combined with PAP at 10 ppm gave highest number of shoots and leaves. MS medium at full strength plus BAP at either 3 or 10 ppm resulted in the highest shoot length and number of leaves.

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Section: Means With the Same Letter In The Same Column Are Not Signifmentioning

confidence: 99%

Tissue Culture Studies on Propagation of Some Ornamental Plants 1. Starting and Shooting Stages of Phytolacca dioica L.

El-Afry

Nofal

Saadawy³

et al. 2017

Journal of Plant Production

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“…For example, weaker salt medium such as WPM promoted the formation of axillary bud development in forest plant species (McCown and Sellmer, 1987) and it was probably due to lower concentration of salts and sucrose than those of B5 and MS (Bhatt and Dhar, 2004). In the other side, application of double strength MS medium resulted in increase the number of formed shoots compared to full strength MS (Gnamien et al, 2013).…”

Section: Introductionmentioning

confidence: 99%

Some important aspects in Moringa oleifera Lam. micropropagation

El-nagish

Hassanein

Salem

2019

AAS

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Type and source of explant as well as the type of cytokinin were important factors for successful moringa micropropagation. Explants obtained from in vitro grown plant materials were better than others obtained from soil growing seedlings. In addition, nodal segments were better than shoot cuttings in terms of number of shoots/ explants, frequency of shoot formation and number of nodes/shoot. While callus formation on the base of nodal segment on BAP containing media were higher than those of KIN, especially under the influence of high concentration as an aspect of vitrification, BAP was better than KIN in moringa multiplication. Low nutrient medium (half strength MS) supplemented with 0.5 mg l-1 IAA was essential for successful root formation. The suitable conditions for moringa micropropagation on full strength MS or SH may exert low stress and low need to raise the expression of SOD and POX. On the other side, stress due to over increase of chemical components of double MS medium or low nutrient content of half strength MS, B5 or WPM expressed the highest number and staining intensity of SOD and POX bands, vice versa was detected in case of CAT.

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Medium effects on micropropagation and genetic stability of <i>Citrullus lanatus</i> oleaginous type

Cited by 2 publications

References 46 publications

Tissue Culture Studies on Propagation of Some Ornamental Plants 1. Starting and Shooting Stages of Phytolacca dioica L.

Tissue Culture Studies on Propagation of Some Ornamental Plants 1. Starting and Shooting Stages of Phytolacca dioica L.

Some important aspects in Moringa oleifera Lam. micropropagation

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