Medium optimization for glutathione production by Saccharomyces cerevisiae

Liu, Chi-Hsien; Hwang, Chin‐Fa; Liao, Chii-Cherng

doi:10.1016/s0032-9592(98)00055-7

Cited by 45 publications

(26 citation statements)

References 16 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Figure 1B presents Comparing the results of biomass and GSH concentrations obtained in the flask culture by other authors using S. cerevisiae strains, the present study obtained very satisfactory results. The highest biomass concentration observed (25.3 g/L) was two times higher compared to other authors (Liu et al 1999;Wen et al 2004;Rollini and Manzoni 2006;Liang et al 2008c). Liu et al (1999) studied GSH production using glucose and peptone with the same Saccharomyces strain and obtained a maximum GSH concentration (124.93 mg/L) similar to the present work (119.6 mg/L).…”

Section: ∑ ∑ ∑contrasting

confidence: 53%

“…The highest biomass concentration observed (25.3 g/L) was two times higher compared to other authors (Liu et al 1999;Wen et al 2004;Rollini and Manzoni 2006;Liang et al 2008c). Liu et al (1999) studied GSH production using glucose and peptone with the same Saccharomyces strain and obtained a maximum GSH concentration (124.93 mg/L) similar to the present work (119.6 mg/L). As the yeast extract and peptone are expensive, the present study applied yeast extract supplemented with whey protein and corn steep liquor.…”

Section: ∑ ∑ ∑contrasting

confidence: 53%

See 1 more Smart Citation

A cost effective fermentative production of glutathione by Saccharomyces cerevisiae with cane molasses and glycerol

Anschau

Santos

Alegre

2013

Braz. arch. biol. technol.

View full text Add to dashboard Cite

This work aimed to evaluate the effect of sugar cane molasses and glycerol on glutathione (GSH) fermentation by Saccharomyces cerevisiae ATCC 7754 in flask culture using response surface methodology. Under optimized conditions (80 g/L of molasses and 60 g/L of glycerol), the highest GSH and biomass concentration achieved were 119.6 mg/L and 25.3 g/L, respectively. Further studies done in 5 L bioreactor resulted 241.3 mg/L GSH after 96 h in batch fermentation without amino acids addition and the concentration of biomass was 12.1 g/L. In batch fermentation with the addition of the three amino acids (4 mM cysteine, glycine and glutamic acid at 32 h), biomass reached to 25 g/L and GSH, 236.1 mg/L at 96 h of fermentation. The strategy of precursor amino acids addition is a key aspect in increasing the synthesis of GSH.

show abstract

Section: ∑ ∑ ∑contrasting

confidence: 53%

Section: ∑ ∑ ∑contrasting

confidence: 53%

A cost effective fermentative production of glutathione by Saccharomyces cerevisiae with cane molasses and glycerol

Anschau

Santos

Alegre

2013

Braz. arch. biol. technol.

View full text Add to dashboard Cite

show abstract

“…In general, glucose is considered the best carbon source. Liu et al (1999) showed glucose to be the best carbon source for the production of glutathione. However, glucose as the carbon source for L. acidophilus A12 resulted in a lower maximum cell density that with lactose, glycerol, mannitol, ethanol, or fructose.…”

Section: Effects Of Various Carbon Nitrogen Sources and Inorganic Smentioning

confidence: 99%

Medium Optimization for the Production of Probiotic Lactobacillus acidophilus A12 Using Response Surface Methodology

Lee¹,

Park²,

Choe³

et al. 2010

Korean Journal for Food Science of Animal Resources

View full text Add to dashboard Cite

Lactobacillus acidophilus A12 was isolated from chicken feces for use as an immunostimulating livestock probiotic. The purpose of this study was to optimize the production of L. acidophilus A12 using response surface methodology (RSM). Initially, the influence of growth medium was studied in terms of carbon sources (glucose, fructose, lactose, glycerol, sucrose, ethanol, and mannitol), nitrogen sources (beef extract, yeast extract, malt extract, and tryptone), and inorganic salts (CaCl , and NaCl). Through one factor-at-a time experiment, lactose, yeast extract, and CaCl 2 were determined to be the best sources of carbon, nitrogen, and inorganic salt, respectively. The optimum composition was found to be 17.7 g/L lactose, 18.6 g/L yeast extract, and 0.9 g/L CaCl 2 . Under these conditions, a maximum cell density of 9.33 Log CFU/mL was produced, similar to the predicted value.

show abstract

“…Sakato and Tanaka [11] used CSL as the nitrogen source in the production media for batch culture of S. cerevisiae KY 6186. In the reports of Udeh and Achremowicz [1] and Liu et al [10], peptone was chosen for GSH production by S. cerevisiae S-8H and BCRC 21727, respectively. They also found that increased peptone level (up to 4%) positively affected the yield of intracellular GSH.…”

Section: Effect Of Nitrogen Sourcementioning

confidence: 99%

“…Being an intracellular product in yeast, various approaches have been adapted to enhance GSH productivity by either increasing the cell biomass or improving GSH content. In addition, various statistic experimental designs were also employed for optimizing the medium composition to maximize the yield of GSH by S. cerevisiae S-8H [1], S. cerevisiae BCRC 21727 [10], and S. cerevisiae T65 [16], respectively. Furthermore, certain amino acids have important effects on both cell growth and GSH biosynthesis in yeast [5, 6, 8, 12 -16].…”

mentioning

confidence: 99%

Co‐production of γ ‐glutamylcysteine and glutathione by mutant strain Saccharomyces cerevisiae FC‐3 and its kinetic analysis

Chen

Huang

Cheng

et al. 2009

J. Basic Microbiol.

View full text Add to dashboard Cite

Co-production of gamma -glutamylcysteine (gamma -GC) and glutathione (GSH) by a novel mutant strain Saccharomyces cerevisiae FC-3 and its kinetic analysis were investigated. The strain could produce gamma -GC and GSH with high yields (4.22 and 14.3 mg/g-DCW, respectively) in batch submerged cultures. Effects of medium components and cultivation conditions on cell growth and the contents of intracellular gamma -GC and GSH were examined. Results show that 2% (w/v) sucrose and 2.5% (w/v) yeast extract are the best carbon and nitrogen sources, respectively, and supplement of three amino acids (glycine, cysteine and glutamate), each at 0.08% (w/v), in the medium could enhance gamma -GC and GSH production. In addition, optimal operating conditions are at the initial pH value of 7.0, 30 degrees C and 200 rev/min. Moreover, results obtained from kinetic analyses reveal that gamma -GC production is mixed-type growth associated, but GSH production is growth-associated.

show abstract

Medium optimization for glutathione production by Saccharomyces cerevisiae

Cited by 45 publications

References 16 publications

A cost effective fermentative production of glutathione by Saccharomyces cerevisiae with cane molasses and glycerol

A cost effective fermentative production of glutathione by Saccharomyces cerevisiae with cane molasses and glycerol

Medium Optimization for the Production of Probiotic Lactobacillus acidophilus A12 Using Response Surface Methodology

Co‐production of γ ‐glutamylcysteine and glutathione by mutant strain Saccharomyces cerevisiae FC‐3 and its kinetic analysis

Contact Info

Product

Resources

About