Medium optimization for keratinase production by a local Streptomyces sp. NRC 13S under solid state fermentation

Shata, Hoda Mohamed Abdel Halim; Farid, Mohamed A.

doi:10.3839/jabc.2013.020

Cited by 3 publications

(4 citation statements)

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“…(13). These results disagree with the results of [16], which found that the best carbon source for maximum keratinase production from Streptomyces sp. under solid-state fermentation was glucose.…”

Section: Effect Of Carbon Sourcecontrasting

confidence: 93%

“…Mazotto,et.al.,[17], showed that the better nitrogen and carbon source for keratinase production from Aspergillus niger was keratin. Other studies that employed other sugars like; starch, sorbitol, lactose, maltose, and sucrose also found increased alkaline keratinase production [9,13,16], suggesting that the optimum carbon source for enzyme production varies depending on the kind of microbe.…”

Section: Effect Of Carbon Sourcementioning

confidence: 96%

“…[15], who demonstrated that submerged fermentation was a better system for the production of keratinase by Aspergillus flavipes. While Shata and Farid [16] found that solid-state fermentation was best system for the production of keratinase from Streptomyces sp. Previously published reports provided supporting evidence that different paradigms of solid substrates, including orange peel, wheat bran, barley, rice straw, sugarcane bagasse, etc., can increase the yield of primary metabolisms in A.terreus [9,13].…”

Section: Type Of Fermentation Systemmentioning

confidence: 99%

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Optimize and Purification of Keratinase Produced from Local Aspergillus Terreus A13 Isolate Using A Feather as Substrate

Al-Sa'ady

2023

Iraqi Journal of Science

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Keratin is a fibrous, insoluble structural protein that is highly cross-linked with hydrophobic, hydrogen, and disulfide bonds. Keratinases are enzymes that belong to the category of serine hydrolases that are capable of breaking down keratin. The results of the determination of the better fermentation system showed that the production of keratinase from local A.terreus A13 isolate by submerged fermentation (SmF) system was the best system to give the highest specific activity (113.4 U/mg) of keratinase compared with solid-state fermentation (SSF). The optimum conditions for keratinase production by SmF, were determined via cultivation conditions, including carbon source, nitrogen source, temperature, pH of the medium, and time of incubation were optimized to enhance the production of total keratinase production in a culture of A.terreus A13 with incubator shaker. The highest product of total keratinase was achieved in feather broth with 2 % sucrose, and 0.5 % soya bean, with a pH of 5.5 at 28 °C for 8 days. Separation and purification of keratinase from a local isolate of A.terreus A13 was done by precipitating with 0-75 % saturated ammonium sulfate, then by ion-exchange chromatography on DEAE-Cellulose column and sephadex G-150 gel. Partially purified keratinase gave an activity of 5.1 U/ml, protein concentration of 0.004 mg/ml, and specific activity of 1275 U/mg with purification fold of 4.96 and 49 % as yield. The aim of the present study was to optimize the production of keratinase from A. terreus A13, cultivated using optimum conditions, and its use for the biodegradation of feathers.

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Section: Effect Of Carbon Sourcecontrasting

confidence: 93%

Section: Effect Of Carbon Sourcementioning

confidence: 96%

Section: Type Of Fermentation Systemmentioning

confidence: 99%

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Optimize and Purification of Keratinase Produced from Local Aspergillus Terreus A13 Isolate Using A Feather as Substrate

Al-Sa'ady

2023

Iraqi Journal of Science

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“…The usual way of handling a dead chicken is by combustion or incineration, burial, composting (for fertilizer) and processing as raw materials for animal or fish (Shata and Farid, 2013). In addition, a technology that is also quite appropriate for the handling of dead chickens is the fermentation technology.…”

Section: Introductionmentioning

confidence: 99%

Effect of different accellerators and inoculums used in fermentation on quality of dead chicken silage flour as feed ingredient for catfish

Bakrie¹,

Sastro²,

Sudolar³

2017

J. Indonesian Trop. Anim. Agric.

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This research aimed at investigating the effect using molasses and cornmeal as additives with Lactobacillus sp. and Streptomyces sp. as inoculums during fermentation on the quality of silage flour made from dead chickens. The study was conducted using a completely randomized factorial design, consisting of 2 factors with 5 replications. The materials used were the newly dead chickens which were chopped and mixed thoroughly with all ingredients; then transferred into a 5 liters plastic box for fermentation. Observations were made after 3 weeks fermentation, including: a) physical characteristics, b) microbial contents, and c) nutritional contents. The data were calculated using variance analysis utilizing computer program of SPSS version 21.0. It was found that based on the protein contents the Lactobacillus sp. (19.0%) was better than the Streptomyces sp. (17.8%) if combined with molasses and corn meal as the accelerators. However, the fat contents produced were relatively similar for both of the inoculums (mean of 37.8%). It can be concluded that in order to obtain a best fermented product in terms of the protein and fat content, the dead chicken should be fermented using molasses and cornmeal as the accelerator and Lactobacillus sp. as the inoculum.

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Keratinases

Kothari

Rani

Goyal

2017

Current Developments in Biotechnology and Bioengineering

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Medium optimization for keratinase production by a local Streptomyces sp. NRC 13S under solid state fermentation

Cited by 3 publications

References 50 publications

Optimize and Purification of Keratinase Produced from Local Aspergillus Terreus A13 Isolate Using A Feather as Substrate

Optimize and Purification of Keratinase Produced from Local Aspergillus Terreus A13 Isolate Using A Feather as Substrate

Effect of different accellerators and inoculums used in fermentation on quality of dead chicken silage flour as feed ingredient for catfish

Keratinases

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