Medium optimization of carbon and nitrogen sources for the production of spores from Bacillus amyloliquefaciens B128 using response surface methodology

Rao, Yerra Koteswara; Tsay, Kuen-Juh; Wu, Wen-Jong; Tzeng, Yew‐Min

doi:10.1016/j.procbio.2006.10.007

Cited by 48 publications

(45 citation statements)

References 22 publications

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“…In the agricultural industry, spores are used as alternatives to agrochemicals as Plant Growth Promoting Rhizobacteria (PGPR) or as Biological Control Agents (BCA) [1][2][3][4]. In the market economy, Bacillus-based products represent about 85 % of the commercially available bacterial BCA [5] but to be able to commercialize these bio-products, industrial exploitation of spores requires high cell density bio-reaction and good sporulation efficiency; for this reason, spore production is a key step in bio-products development when AEFB are the active ingredient [6].…”

Section: Introductionmentioning

confidence: 99%

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Effect of medium components and culture conditions in Bacillus subtilis EA-CB0575 spore production

Posada-Uribe

Romero-Tabarez

Villegas-Escobar

2015

Bioprocess Biosyst Eng

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Bacillus subtilis spores have important biotechnological applications; however, achieving both, high spore cell densities and sporulation efficiencies in fermentation, is poorly reported. In this study, medium components and culture conditions were optimized with different statistical methods to increase spore production of the plant growth promoting rhizobacteria B. subtilis EA-CB0575. Key medium components were determined with Plackett-Burman (PB) design, and the optimum concentration levels of two components (glucose, MgSO4·7H2O) were optimized with a full factorial and central composite design, achieving 1.37 × 10(9) CFU/mL of spore cell density and 93.5 % of sporulation efficiency in shake flask. The optimized medium was used to determine the effect of culture conditions on spore production at bioreactor level, finding that maintaining pH control did not affect significantly spore production, while the interaction of agitation and aeration rates had a significant effect on spore cell density. The overall optimization generated a 17.2-fold increase in spore cell density (8.78 × 10(9) CFU/mL) and 1.9-fold increase in sporulation efficiency (94.2 %) compared to that of PB design. These results indicate the potential of B. subtilis EA-CB0575 to produce both, high spore cell densities and sporulation efficiencies, with very low nutrient requirements and short incubation period which can represent savings of process production.

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Section: Introductionmentioning

confidence: 99%

“…30-80 %, respectively using serial dilution and plate count methods [11][12][13][14]. Other studies have reported higher sporulation efficiencies or higher spore cell densities for other Bacillus species different from B. subtilis [3,6,15,16], but to our knowledge obtaining high values of both variables has not been reported yet.…”

Section: Introductionmentioning

confidence: 99%

Effect of medium components and culture conditions in Bacillus subtilis EA-CB0575 spore production

Posada-Uribe

Romero-Tabarez

Villegas-Escobar

2015

Bioprocess Biosyst Eng

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“…The final spore concentration obtained was higher than those reported by many authors; for example, Rao and colleagues [14] had a final concentration of 5.93 × 10 8 spores/ml in the optimization of a culture medium for the production of spores of B. amyloliquefaciens B128, whereas Warriner and Walter [21] obtained a concentration of 3.0 × 10 9 spores/ml with the use of simple carbohydrates in the production of spores of B. subtilis. With respect to pH, a final value of 8.06 was obtained because after the depletion of the carbon source (46 hours), the medium began to alkalize, probably because of the reuptake of organic acids and deamination of peptones [21].…”

Section: Validation Of the Best Treatment In Liquid Fermentationmentioning

confidence: 55%

“…The salts contained in the mixture have been one of the most frequently found in the culture media optimization for the production of spores [12]- [14]; because they are involved in the gene expression for dipicolinic acid synthesis, a specific component of bacterial endospores which is responsible of spore heat resistance [15]- [17].…”

Section: Sporulation Induction With Individual Factorsmentioning

confidence: 99%

Improvement of Sporulation Conditions of a New Strain of <i>Bacillus amyloliquefaciens</i> in Liquid Fermentation

Díaz-García¹,

García-Riaño²,

Zapata-Narvaez³

2015

ABB

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The desirable active ingredients for the development of bioproducts based in Bacillus sp. for the control of soil pathogens are the spores because these structures exhibit more resistance and stability to conditions present during the fermentation, formulation, and storage processes. To improve the sporulation of a native strain of Bacillus amyloliquefaciens (Bs006) using liquid fermentation, some modifications in the concentrations of the components in a previously standardized culture media were made. Subsequently, five sporulation inducers (iron nitrate, mixture of salts, peroxide hydrogen, temperature, and initial cell concentration) were evaluated. The treatment with a mixture of salts in combination with an initial cell concentration of 1 × 10 8 cells/ml was selected because a final spore concentration of 1.05 × 10 10 spores/ml after 66 hours with a fully substrate consumption and sporulation efficiency of 88.7% was obtained. To demonstrate the biological activity of B. amyloliquefaciens Bs006 in Cape gooseberry seedlings, a greenhouse bioassay was conducted, and statistical differences in plant growth-promoting parameters compared with previous media were not found. Additionally, the proposed modified media (coded as JM) presented a benefit-cost ratio 2.65 times higher compared with the baseline media.

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“…It is an efficient mathematical approach widely applied in the optimization of the fermentation process and media component, e.g., production of enzymes, biomass, spore, and other metabolites [12][13][14][15] . RSM, which includes factorial design and regression analysis, can be used to help evaluate the effective factors www.scienceasia.org and build models 16 .…”

Section: Introductionmentioning

confidence: 99%

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Romsomsa¹,

Chim-anagae²,

Jangchud³

2010

ScienceAsia

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Bacillus subtilis C4 was isolated from waste water of a Thai-silk dyeing factory, and identified as a potent strain for producing silk-degumming protease. To optimize the protease production of this bacterial strain, seven fermentation variables were screened using a Plackett-Burman design, and were then further optimized via response surface methodology based on a central composite design. Three significant variables, i.e., soy flour, skimmed milk, and shaker speed, were selected. The optimal values were 2.0% soy flour, 0.1% skimmed milk, and a shaker speed of 280 rpm. The experimental result (1537 units/ml) in a medium optimized for protease production was in good agreement with the predicted value of a quadratic model (1576 units/ml), thus confirming its validity. In addition, the adequacy of the model was supported by a coefficient of determination (R 2 ) of 0.912. Protease production in the optimized medium (1537 units/ml) increased 2.2-fold over that of the non-optimized medium (729 units/ml) in the shaken flask culture. When the experiment was scaled up in a stirred tank reactor, 1891 units/ml protease activity was achieved at 27 h of cultivation, which was an overall 2.6-fold increase over the basal medium.

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Medium optimization of carbon and nitrogen sources for the production of spores from Bacillus amyloliquefaciens B128 using response surface methodology

Cited by 48 publications

References 22 publications

Effect of medium components and culture conditions in Bacillus subtilis EA-CB0575 spore production

Effect of medium components and culture conditions in Bacillus subtilis EA-CB0575 spore production

Improvement of Sporulation Conditions of a New Strain of <i>Bacillus amyloliquefaciens</i> in Liquid Fermentation

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Medium optimization of carbon and nitrogen sources for the production of spores from Bacillus amyloliquefaciens B128 using response surface methodology

Cited by 48 publications

References 22 publications

Effect of medium components and culture conditions in Bacillus subtilis EA-CB0575 spore production

Effect of medium components and culture conditions in Bacillus subtilis EA-CB0575 spore production

Improvement of Sporulation Conditions of a New Strain of &lt;i&gt;Bacillus amyloliquefaciens&lt;/i&gt; in Liquid Fermentation

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Improvement of Sporulation Conditions of a New Strain of <i>Bacillus amyloliquefaciens</i> in Liquid Fermentation