Medium-term and long-term in vitro conservation and safe international exchange of yam (<i>Dioscorea spp.</i>) germplasm

Malaurie, Bernard; Trouslot, Marie-France; Berthaud, Julien; Bousalem, M.; Pinel, Alexandre; Dubern, Jean‐Frédéric

doi:10.2225/vol1-issue3-fulltext-2

Cited by 24 publications

(13 citation statements)

References 13 publications

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“…De acordo com Malaurie et al (1998), cerca de 20 espécies de inhame são conservadas no Institut de Recherche pour le Développement (IRD) sob condições de crescimento mínimo.…”

Section: Introductionunclassified

Conservação in vitro de germoplasma de cana-de-açúcar

Lemos

Ferreira

Alencar

et al. 2002

Pesq. agropec. bras.

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Resumo -O objetivo deste trabalho foi avaliar o efeito da temperatura, de sacarose, manitol e sorbitol, como fontes de carbono e reguladores osmóticos, e do ácido abscísico, como regulador de crescimento na conservação in vitro de germoplasma de cana-de-açúcar. Foram utilizadas, como material vegetal, gemas apicais de plantas de 10 meses de idade, do banco de germoplasma in vivo, da Universidade Federal de Alagoas. Brotos da quarta repicagem, no estádio de multiplicação in vitro, foram a fonte de explantes para três experimentos. Houve efeito positivo da diminuição da temperatura e da utilização da sacarose como fonte de carbono e regulador osmótico na manutenção da viabilidade dos explantes conservados in vitro. O ácido abscísico (1 mg/L) foi essencial para manter os explantes em crescimento mínimo por 12 meses (52 semanas). O uso das concentrações de 1 mg/L de ácido abscísico e de 20 g/L de sacarose associadas às condições de temperatura reduzida (15°C) demonstraram que os brotos permaneceram viáveis por um ano no mesmo meio de cultura, sem a necessidade de serem subcultivados.Termos para indexação: Saccharum, explante, cultura de tecido, micropropagação, melhoramento de plantas. In vitro conservation of sugarcane germplasmAbstract -The objective of this work was to evaluate the effect of temperature, of sucrose, sorbitol and manitol, as carbon source and osmotic regulator, and of abscisic acid, as growth regulator, on in vitro germplasm conservation of sugarcane. Stem tips of 10 month old plants collected from the sugarcane germplasm bank of the Universidade Federal de Alagoas in Brazil were introduced in vitro and secondary shoots were produced in a multiplication medium. New shoots collected after four subcultures were used in three conservation experiments. There was a positive effect of the low temperature and sucrose as an osmotic regulator and carbon source in maintaining the viability of the explants cultured in vitro. Abscisic acid (1 mg/L) was essential to maintain the explants in a reduced growth condition for 52 weeks without any subculture. The explants promptly returned to normal growth in vitro or were readily acclimatized after 52 weeks of conservation, in the medium with abscisic acid (1 mg/L) plus sucrose (20 g/L) at 15ºC. IntroduçãoO melhoramento de plantas tem contribuído para o aumento da produção em diversas culturas comerciais de grande relevância em todo o mundo. A obtenção de variedades de alto rendimento e resistência a pragas e doenças requer que os recursos genéticos desejáveis de determinada espécie encontrem-se disponíveis para sua utilização. Com isso, as coleções de germoplasma passam a ter papel fundamental nos programas de melhoramento (Silva et al., 1997).A conservação das coleções de cana-de-açúcar é comercialmente feita mediante o cultivo de touceiras em campo. Os elevados custos de manutenção, os riscos de perda por intempéries, pragas e enfermidades, tornam o banco de germoplasma in vitro um meio bastante atrativo (Withers, 1991).A conservação de plantas in vitro se base...

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“…De acordo com Malaurie et al (1998), cerca de 20 espécies de inhame são conservadas no Institut de Recherche pour le Développement (IRD) sob condições de crescimento mínimo.…”

Section: Introductionunclassified

Conservação in vitro de germoplasma de cana-de-açúcar

Lemos

Ferreira

Alencar

et al. 2002

Pesq. agropec. bras.

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“…Pertumbuhan tinggi tanaman pada media MS yang mengandung manitol 40 dan 50 g/l sampai umur 24 minggu masih lambat sehingga belum diperlukan subkultur. Interval subkultur yang lama akan menghemat biaya, mengurangi laju kontaminasi dan menghindarkan terjadinya mutasi pada plasma nutfah yang disimpan (Malaurie et al, 1998).…”

Section: Tinggi Tanamanunclassified

Perbanyakan dan Konservasi In Vitro Plasma Nutfah Talas (Colocasia esculenta (L.) Schoot)

et al. 2016

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In Vitro Micropropagation and Conservation of Taro (Colocasia esculenta (L.) Schott). Nurwita Dewi, Bambang S. Purwoko, Ida Hanarida, Agus Purwito, and Iswari S. Dewi. Taro is a potential source of carbohydrate for anticipation of climate change. In vitro technology have not been widely implemented for tuber crops conservation. Conservation of the crops is mostly conducted in field. Such conservation is very susceptible to biotic and abiotic stress. The research consisted of two activities i.e: micropropagation and conservation. The objectives were to obtain taro in vitro propagation and conservation method. The trial was arranged in a factorial design with six replications. Five taro accessions were used as the first factor for each study. The second factor in propagation study was propagation medium i.e: MS; MS + 2.9 μM IAA + 4.4 μM BA and MS + 2.9 μM IAA + 22,2 μM BA. Shoot tip from taro sucker was used as explant. The second factor in conservation study was MS medium containing mannitol (0, 30, 40, and 50 g/l). Twoleaves in vitro shoots from micropropagation study was used as explants. The addition of BA in MS medium + 2.9 μM IAA increased the number of shoot of taro germplasm. The best medium for micropropagation of taro germplasm No. 21 and Talas Jahe is MS + 2,9 μM IAA + 4,4 μM BA, whereas the best medium for No. 503, Talas Jahe and Lumbu Banten is MS + 2,9 μM IAA + 22,2 μM BA. Based on data of plant height, percentage of leaf life and shelf life, MS medium + manitol 40 g/l was the best medium for taro germplasm conservation with prolong sub-culture interval.

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“…Sucrose concentrations range from 0.3 to 1 M and treatment is for 16-4 weeks. In the encapsulation-dehydration protocol sugar concentrations up to 1 M are applied (Malaurie et al 1998a). For lily (Bouman et al 2003) and garlic (Kim et al 2004a), cold acclimation and sugar hardening are applied simultaneously.…”

Section: Asparagusmentioning

confidence: 99%

“…The encapsulation-dehydration method was successfully applied to Cynodon, Zoysia, and Lolium grasses ), sugarcane (González-Arnao et al 1993Paulet et al 1993), yam (Malaurie et al 1998a;Malaurie et al 1998b;Mandal et al 1996), lily and banana (Panis 1995) After encapsulation in alginate beads, meristems are exposed to liquid medium with 0.75 M sucrose. In yam, sugar concentrations are increased step-by-step to 0.9 or 1 M, then beads are dehydrated in a container with dry silica gel or in the laminar airflow bench to achieve moisture contents between 19% and 26% and then plunged into LN (Chap.…”

Section: Cryoprotection and Cryopreservationmentioning

confidence: 99%

Plant Cryopreservation: A Practical Guide

Reed¹

2008

198

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Medium-term and long-term in vitro conservation and safe international exchange of yam (Dioscorea spp.) germplasm

Cited by 24 publications

References 13 publications

Conservação in vitro de germoplasma de cana-de-açúcar

Conservação in vitro de germoplasma de cana-de-açúcar

Perbanyakan dan Konservasi In Vitro Plasma Nutfah Talas (Colocasia esculenta (L.) Schoot)

Plant Cryopreservation: A Practical Guide

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