Meiotic irregularities in Capsicum L. species

Souza, Margarete Magalhães; Pereira, Telma Nair Santana; Sudré, Cláudia Pombo; Rodrigues, Rosana

doi:10.1590/s1984-70332012000200007

Cited by 8 publications

(6 citation statements)

References 26 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Different chromosomal association such as univalent, trivalent, and tetravalent (evidence for structural change) found in this study suggest similar genetic structure between the species involved in the crossing and are the most common for Passi ora species (Souza et al 2012). Furthermore, these irregular chromosome pairing con gurations did not interfere with regular chromosome segregation at the end of meiosis, because, there was the presence of at least one chiasmas per bivalent, con rming chromosomal pairing in hybrid genotypes.…”

Section: Discussionsupporting

confidence: 48%

See 1 more Smart Citation

Meiosis and male fertility in F1 interspecific hybrids (Passiflora vitifolia vs. Passiflora hatschbachii)

Souza

Silva

et al. 2022

Euphytica

Self Cite

View full text Add to dashboard Cite

Interspeci c hybrids can be studied using methodologies in which the male gamete with high reproduction potential, viability, and fertility is prioritized. Passi ora species, with lush, showy, and exotic colors, have great potential for the ornamental plant market. In addition, arti cial Passi ora hybrids were developed without many di culties because of weak reproductive barriers. Thus, meiotic and post-meiotic behaviors were analyzed with 2% acetic carmine staining. Con rmation of interspeci c hybridization was performed using SSR markers and GISH technique was used to detect genomic differentiation. The pollen viability of the parental and hybrids genotypes was tested using Alexander solution, uorescein diacetate and in vitro germination tests were performed using culture medium. The meiotic behavior was regular and displayed haploid number n = 9 with nine bivalent (II) chromosomal, pairing in 90% of the cells in diakinesis. There was a signi cant difference (p < 0.05) in terminal and interstitial chiasma frequencies. Meiotic irregularities observed were as follows: early and/or delayed chromosomes, disorientation of spindle bers, transverse spindles, tripolar spindles, and asynchrony; and consequently irregular post-meiotic products were observed: monads, dyads, triads, and polyads. GISH was used in the interspeci c hybrids and pairing between homeologous chromosomes, and bivalent and tetravalent formation were observed. From this study, we could conclude that hybrid genotypes are fertile and pollen grains are viable and can be used in breeding programs. We also hypothesize that interspeci c hybrid genotypes of Passi ora can be obtained with regular meiosis, which could be viable and fertile.

show abstract

Section: Discussionsupporting

confidence: 48%

“…However, ampli cation of a few primers can con rm interspeci c crossings. A speci c locus of each genitor present in the hybrid progeny was su cient to con rm hybridization (Conceição et al 2011;Souza et al 2012;Melo et al 2016).…”

Section: Discussionmentioning

confidence: 99%

Meiosis and male fertility in F1 interspecific hybrids (Passiflora vitifolia vs. Passiflora hatschbachii)

Souza

Silva

et al. 2022

Euphytica

Self Cite

View full text Add to dashboard Cite

show abstract

“…Genetic diversity (H e ) was estimated from the 6,426 C. annuum polymorphic SPP markers using PowerMarker [53]. H e is defined as the probability that two randomly chosen alleles from a population are different [54].…”

Section: Methodsmentioning

confidence: 99%

Characterization of Capsicum annuum Genetic Diversity and Population Structure Based on Parallel Polymorphism Discovery with a 30K Unigene Pepper GeneChip

et al. 2013

View full text Add to dashboard Cite

The widely cultivated pepper, Capsicum spp., important as a vegetable and spice crop world-wide, is one of the most diverse crops. To enhance breeding programs, a detailed characterization of Capsicum diversity including morphological, geographical and molecular data is required. Currently, molecular data characterizing Capsicum genetic diversity is limited. The development and application of high-throughput genome-wide markers in Capsicum will facilitate more detailed molecular characterization of germplasm collections, genetic relationships, and the generation of ultra-high density maps. We have developed the Pepper GeneChip® array from Affymetrix for polymorphism detection and expression analysis in Capsicum. Probes on the array were designed from 30,815 unigenes assembled from expressed sequence tags (ESTs). Our array design provides a maximum redundancy of 13 probes per base pair position allowing integration of multiple hybridization values per position to detect single position polymorphism (SPP). Hybridization of genomic DNA from 40 diverse C. annuum lines, used in breeding and research programs, and a representative from three additional cultivated species (C. frutescens, C. chinense and C. pubescens) detected 33,401 SPP markers within 13,323 unigenes. Among the C. annuum lines, 6,426 SPPs covering 3,818 unigenes were identified. An estimated three-fold reduction in diversity was detected in non-pungent compared with pungent lines, however, we were able to detect 251 highly informative markers across these C. annuum lines. In addition, an 8.7 cM region without polymorphism was detected around Pun1 in non-pungent C. annuum. An analysis of genetic relatedness and diversity using the software Structure revealed clustering of the germplasm which was confirmed with statistical support by principle components analysis (PCA) and phylogenetic analysis. This research demonstrates the effectiveness of parallel high-throughput discovery and application of genome-wide transcript-based markers to assess genetic and genomic features among Capsicum annuum.

show abstract

“…According to Pagliarini (2000), irregular chromosome segregation is the most common meiotic abnormality in both cultivated and native species, being characterized by the presence of chromosomes in precocious chromosome ascension and laggard choromosome. These abnormalities have been reported in Zeas mays (Ricci et al 2007), Saccharum officinarum (Pagliarini et al 1990), Avena sativa (Baptista-Giacomelli et al 2000), in species of the genus Paspalum Dahmer et al 2008), in genus Capsicum L. (Souza et al 2012), and in Brachiaria (Boldrini et al 2009, Boldrini et al 2011, Mendes-Bonato et al 2006.…”

Section: Krb Tolomeotti Et Almentioning

confidence: 64%

“…The presence of univalent in diakinesis and precocious chromosome migration to the poles in metaphase I may be a result of the precocious chiasma terminalization, causing the migration of precocious chromosomes to the poles, since chiasmas are generally responsible for the maintenance of the bivalents for the perfect segregation occurrence (Singh 1993, Pagliarini 2000, or due to the presence of synaptic or disynaptic genes in prophase I (Koduru and Rao 1981). In Capsicum baccatum accession, small chromosome alterations can occur in the process of domestication and improvement (Souza et al 2012).…”

Section: Krb Tolomeotti Et Almentioning

confidence: 99%

Cytogenetic characterization of Tropaeolum pentaphyllum Lam

Tolomeotti

Felippi

Donazzolo

2018

Crop Breed. Appl. Biotechnol.

View full text Add to dashboard Cite

The species Tropaeolum pentaphyllum Lam. (batata-crem or crem) is of great economic and medicinal importance. For being an endangered species, the knowledge of its genetic variability is necessary. Considering the lack of cytological studies on this species, this work describes the chromosome numbers, the meiotic behavior, the meiotic index, and the viability of the pollen grains of three populations of T. pentaphyllum from Rio Grande do Sul, Brazil. The three populations presented 2n = 28 chromosomes. Meiotic behavior was regular, with low abnormality frequency (<1%), which culminated in IMx> 90% in the three accessions. This meiotic stability resulted in high production of viable pollen grains, which will favor the use of these accessions in selection programs for commercial purposes, conservation and sustainable use.

show abstract

Meiotic irregularities in Capsicum L. species

Cited by 8 publications

References 26 publications

Meiosis and male fertility in F1 interspecific hybrids (Passiflora vitifolia vs. Passiflora hatschbachii)

Meiosis and male fertility in F1 interspecific hybrids (Passiflora vitifolia vs. Passiflora hatschbachii)

Characterization of Capsicum annuum Genetic Diversity and Population Structure Based on Parallel Polymorphism Discovery with a 30K Unigene Pepper GeneChip

Cytogenetic characterization of Tropaeolum pentaphyllum Lam

Contact Info

Product

Resources

About