Melatonin: a growth-stimulating compound present in lupin tissues

is an indoleamine which is structurally related to tryptophan, serotonin and indole-3-acetic acid (IAA), among other important substances. Many studies have clearly demonstrated its presence in different plant organs, including roots, stems, leaves, flowers, fruits and seeds. Since it discovery in plants in 1995, authors have postulated many physiological roles for melatonin, although research into this molecule in plants is still in its infancy. The data presented in this study demonstrate that melatonin stimulates the expansion of etiolated cotyledons of lupin (Lupinus albus L.) to a similar extent to that observed for IAA but less than in the case of kinetin. Endogenous melatonin in imbibed cotyledons has been quantified using a liquid chromatography method with fluorescence detection and capacity of cotyledons to absorb melatonin has been determined. The observed effect of melatonin on lupin cotyledon expansion can be added to the other effects demonstrated by our group such as its role as growth promoter and rooting promotor in adventitious and lateral roots.

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“…1) (Hernández-Ruiz et al 2004). Melatonin and IAA were also seen to be distributed in lupin tissues in a similar concentration gradient.…”

Section: Introductionmentioning

confidence: 73%

Melatonin stimulates the expansion of etiolated lupin cotyledons

2008

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“…Melatonin extraction was done at 4°C according to the modified methods of Guerrero et al [26] and HernandezRuiz et al [27]. Lyophilized V. radiata roots (0.3 g) were homogenized with chilled K-phosphate buffer (pH 8.0), supplemented with EDTA and BHT, shaken horizontally (darkness/room temperature/15 min) and centrifuged (20 000 x g/5°C/10 min).…”

Section: Melatonin Extraction and Assaymentioning

confidence: 99%

Involvement of melatonin applied to Vigna radiata L. seeds in plant response to chilling stress

2014

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The impact of melatonin (50 µM L−1) applied to Vigna radiata seeds by hydro-priming on phenolic content, L-phenylalanine ammonialyase (PAL) activity, MEL level, antioxidant properties of ethanol extracts as well as electrolyte leakage from chilled and re-warmed Vigna radiata roots of seedlings were examined. Seedlings obtained from non-primed seeds, hydro-primed and hydro-primed with MEL were investigated after 2 days of chilling and 2 days of re-warming. At 25°C, the level of MEL in roots derived from seeds hydro-primed with MEL was 7-fold higher than in roots derived from non-primed seeds. However, the content of MEL significantly decreased in all variants investigated after re-warming, in contrast to PAL activity and phenolic levels, which reached the highest values. The antioxidant capacity of ethanol extracts from chilled and re-warmed roots, determined by ABTS+· assay, was correlated with phenolic content while the reducing ability of these extracts, determined by the FRAP method, correlated with PAL activity. However, both were the highest in rewarmed roots with applied MEL, which was accompanied by a significant decline in electrolyte leakage. Taken together, results may indicate that MEL can play a positive role in plant acclimation to stressful conditions and activation of phenolic pathway by this molecule.

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“…The significance of these indoleamines in plant physiology may be related to auxin metabolism. 11 Similarly, SER is reported stimulates the germination of radish seeds, 24 and the pollen of Hippeastrum hybridum. 25 Therefore our finding of high levels of SER/MEL in seeds ( Table 4) may be of significance in this context.…”

Section: Resultsmentioning

confidence: 85%

“…Ravishankar* role in various developmental aspects of plant morphogenesis is worth investigating. 11 Interestingly, high concentrations of SER is found in organs of movements, the pulvini and tendrils of Albizzia julibrissin, Pisum sativum, Mimosa pudica and Passiflora quadrangularis compared with other vegetative parts. 12 SER is implicated in adaptation to environmental changes, flowering, morphogenesis and ion permeability.…”

Section: Introductionmentioning

confidence: 98%