Melatonin acts through different mechanisms to control oxidative stress and primordial follicle activation and survival during in vitro culture of bovine ovarian tissue

Silva, Bianca R.; Costa, Francisco C.; De Lima Neto, Miguel F.; Caetano Filho, Francisco F.; de Assis, Ernando I.T.; Aguiar, Francisco L.N.; Silva, Anderson W.B.; Martins, Solano D.; Araújo, Valdevane R.; Matos, Maria H.T.; Costa, José J.N.; Silva, José R.V.

doi:10.1016/j.domaniend.2023.106824

Cited by 6 publications

(3 citation statements)

References 60 publications

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“…In vitro culture of ovarian tissues in control medium results in a significant reduction in the expression of mRNA for NRF2, SOD, CAT, GPX1, and PRDX6. Similar results were also reported recently by Silva et al [7]. An imbalance in oxidant/antioxidant signaling due to an increase in supraphysiological ROS levels was also reported in in vitro cultured oocytes and embryos [28].…”

Section: Discussionsupporting

confidence: 91%

“…Collagen, elastin, fibronectin, and laminin are compounds present in the ECM that form a structurally stable structure that works as a reservoir of hormones and growth factors, regulating their diffusion and availability within the ovarian environment [3]. Despite advances in understanding the control of early follicular development, culture of ovarian tissues is still associated with high production of reactive oxygen species (ROS), which trigger oxidative stress [4] and, consequently, result in high follicular degeneration [5][6][7].…”

Section: Introductionmentioning

confidence: 99%

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α-Pinene Improves Follicle Morphology and Increases the Expression of mRNA for Nuclear Factor Erythroid 2-Related Factor 2 and Peroxiredoxin 6 in Bovine Ovarian Tissues Cultured In Vitro

Azevedo,

De Assis,

Silva

et al. 2024

Animals

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Oxidative stress during in vitro of ovarian tissues has adverse effects on follicle survival. α-pinene is a monoterpenoid molecule with antioxidant activity that has great potential to maintain cell survival in vitro. This study investigated the effect of α-pinene (1.25, 2.5, 5.0, 10.0, or 20.0 μg/mL) on primordial follicle growth and morphology, as well as on stromal cells and collagen fibers in bovine ovarian slices cultured for six days. The effect of α-pinene on transcripts of catalase (CAT), superoxide dismutase (SOD), peroxiredoxin 6 (PRDX6), glutathione peroxidase (GPX1), and nuclear factor erythroid 2-related factor 2 (NRF2) was investigated by real-time PCR. The tissues were processed for histological analysis to evaluate follicular growth, morphology, stromal cell density, and collagen fibers. The results showed that 2.5, 5.0, or 10.0 µg/mL α-pinene increased the percentages of normal follicles but did not influence follicular growth. The α-pinene (10.0 µg/mL) kept the stromal cell density and collagen levels in cultured bovine ovarian tissue like uncultured tissues. Ovarian tissues cultured in control medium had reduced expression of mRNA for NRF2, SOD, CAT, GPX1, and PRDX6, but α-pinene (10.0 µg/mL) increased mRNA levels for NRF2 and PRDX6. In conclusion, 10.0 µg/mL α-pinene improves the follicular survival, preserves stromal cell density and collagen levels, and increases transcripts of NRF2 and PRDX6 after in vitro culture of bovine ovarian tissue.

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Section: Discussionsupporting

confidence: 91%

Section: Introductionmentioning

confidence: 99%

α-Pinene Improves Follicle Morphology and Increases the Expression of mRNA for Nuclear Factor Erythroid 2-Related Factor 2 and Peroxiredoxin 6 in Bovine Ovarian Tissues Cultured In Vitro

Azevedo,

De Assis,

Silva

et al. 2024

Animals

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“…This characteristic has unveiled a new realm of possibilities, suggesting that besides its receptormediated hormonal actions, melatonin may also exert receptor-independent effects in regulating reproduction (Gao et al 2022). Melatonin critically scavenges oxygen free radicals during oocyte maturation and ovulation, mitigating the detrimental effects of reactive oxygen species (ROS) and protecting oocytes from oxidative damage (Silva et al 2024). Ovarian melatonin follows a seasonal pattern in carp, reaching its peak at spawning and this temporal variation shows a signi cant negative correlation with malondialdehyde (MDA) level, an indicator of intracellular oxidative stress (Hasan et al 2014).…”

Section: Introductionmentioning

confidence: 99%

Correlation of testicular melatonin and cellular stress in an annual spermatogenic cycle of Clarias batrachus

Acharyya,

Hasan

2024

Preprint

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Melatonin, primarily produced by pineal gland, shows a rhythmic pattern of synthesis in response to environmental cues. Hitherto, the role of testicular melatonin in the regulation of annual spermatogenic cycle is indeterminate in any fish species. Present study aims to explore a correlation, for the first time, of melatonin with cellular stress levels in accordance to the rhythmic progression of spermatogenesis through six distinct reproductive phases of an annual gonadal cycle of catfish, Clarias batrachus under natural photo-thermal conditions. We assessed gonadosomatic index (GSI), testicular melatonin concentrations, relative percentage of haploid cells by using DNA dye in the seminiferous tubules, and different intracellular stress markers. The concentration of testicular melatonin displayed a distinct seasonal pattern, reaching their peak during the “phase of functional maturity”. Interestingly, the levels of 2’,7’–dichlorofluorescein [reactive oxygen species (ROS) marker], malondialdehyde (MDA) and RNS (total nitrate) were found significantly lowered at the verge of “mature state”. Correlation and LOESS regression analyses showed a striking positive relationship between testicular melatonin concentration and the activity of antioxidative enzymes, including superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GPx) and glutathione S-transferase (GST). Principal component (PC) analysis also identified the key components of “mature state” viz., GSI, testicular melatonin, haploid cell population (spermatid and spermatozoa), and the activity of enzymatic antioxidants. Cumulatively, melatonin may have a role in growth and maturation of spermatogenic cells by reducing the cellular stress to augment the quality of germ cells required for seed production in the culture of any fish species.

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Improving survival and growth of caprine preantral follicles cultured in medium commonly used for MSC: Role of oxidative stress regulation and epigenetic changes

Silva,

Lima,

Ferreira

et al. 2025

Domestic Animal Endocrinology

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Melatonin acts through different mechanisms to control oxidative stress and primordial follicle activation and survival during in vitro culture of bovine ovarian tissue

Cited by 6 publications

References 60 publications

α-Pinene Improves Follicle Morphology and Increases the Expression of mRNA for Nuclear Factor Erythroid 2-Related Factor 2 and Peroxiredoxin 6 in Bovine Ovarian Tissues Cultured In Vitro

α-Pinene Improves Follicle Morphology and Increases the Expression of mRNA for Nuclear Factor Erythroid 2-Related Factor 2 and Peroxiredoxin 6 in Bovine Ovarian Tissues Cultured In Vitro

Correlation of testicular melatonin and cellular stress in an annual spermatogenic cycle of Clarias batrachus

Improving survival and growth of caprine preantral follicles cultured in medium commonly used for MSC: Role of oxidative stress regulation and epigenetic changes

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