Melatonin alters neuronal architecture and increases cysteine‐rich protein 1 signaling in the male mouse hippocampus

Ang, Mary Jasmin; Kang, Sohi; Moon, Changjong

doi:10.1002/jnr.24708

Cited by 8 publications

(11 citation statements)

References 97 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Golgi staining was used to visualize the dendritic branching complexity and spines of the neurons in the mice hippocampi. The methods and protocols employed were previously described [ 30 , 58 ]. The FD Rapid Golgistain™ Kit (FD Neurotechnologies, Ellicott City, MD, USA) was used, according to the manufacturer’s instructions.…”

Section: Methodsmentioning

confidence: 99%

“…The methods for quantifying dendritic complexity, dendritic length, and the number of branch points were described for our previous studies [ 30 , 58 ] with some modifications. Briefly, Sholl’s concentric method [ 59 ] used with the ImageJ (National Institutes of Health, Bethesda, MD, USA) program was used to analyze the neuronal tracings visualized by the 200× Leica DM750 optical microscope (Leica Microsystems, Wetzlar, Germany) and traced using the Leica Application Suite (version 4.12, Leica Microsystems) and Adobe Photoshop CS6 (Adobe Systems, San Jose, CA, USA).…”

Section: Methodsmentioning

confidence: 99%

“…The measurement of dendritic spine density and morphology was performed according to our previous studies [ 20 , 58 ]. Briefly, all protruding dendritic spines were counted on 30 µm dendritic segments.…”

Section: Methodsmentioning

confidence: 99%

“…The immunofluorescence protocol was based on our previous study with some modifications [ 58 ]. The brains were collected and perfused with 4% ( w / v ) paraformaldehyde (Sigma-Aldrich, St. Louis, MO, USA) and transferred into 30% ( w / v ) sucrose solution for 4 days, and 30 µm-thick coronal sections were made using a frozen sliding microtome (SM2010R; Leica Microsystems) at around −2.06 mm relative to the bregma.…”

Section: Methodsmentioning

confidence: 99%

See 3 more Smart Citations

SREBP-1c Deficiency Affects Hippocampal Micromorphometry and Hippocampus-Dependent Memory Ability in Mice

Ang

Lee

Wada

et al. 2021

IJMS

Self Cite

View full text Add to dashboard Cite

Changes in structural and functional neuroplasticity have been implicated in various neurological disorders. Sterol regulatory element-binding protein (SREBP)-1c is a critical regulatory molecule of lipid homeostasis in the brain. Recently, our findings have shown the potential involvement of SREBP-1c deficiency in the alteration of novel modulatory molecules in the hippocampus and occurrence of schizophrenia-like behaviors in mice. However, the possible underlying mechanisms, related to neuronal plasticity in the hippocampus, are yet to be elucidated. In this study, we investigated the hippocampus-dependent memory function and neuronal architecture of hippocampal neurons in SREBP-1c knockout (KO) mice. During the passive avoidance test, SREBP-1c KO mice showed memory impairment. Based on Golgi staining, the dendritic complexity, length, and branch points were significantly decreased in the apical cornu ammonis (CA) 1, CA3, and dentate gyrus (DG) subregions of the hippocampi of SREBP-1c KO mice, compared with those of wild-type (WT) mice. Additionally, significant decreases in the dendritic diameters were detected in the CA3 and DG subregions, and spine density was also significantly decreased in the apical CA3 subregion of the hippocampi of KO mice, compared with that of WT mice. Alterations in the proportions of stubby and thin-shaped dendritic spines were observed in the apical subcompartments of CA1 and CA3 in the hippocampi of KO mice. Furthermore, the corresponding differential decreases in the levels of SREBP-1 expression in the hippocampal subregions (particularly, a significant decrease in the level in the CA3) were detected by immunofluorescence. This study suggests that the contributions of SREBP-1c to the structural plasticity of the mouse hippocampus may have underlain the behavioral alterations. These findings offer insights into the critical role of SREBP-1c in hippocampal functioning in mice.

show abstract

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

See 2 more Smart Citations

SREBP-1c Deficiency Affects Hippocampal Micromorphometry and Hippocampus-Dependent Memory Ability in Mice

Ang

Lee

Wada

et al. 2021

IJMS

Self Cite

View full text Add to dashboard Cite

show abstract

“…Immunohistochemical analysis of free-floating sections was performed as per protocols described in previous studies [32][33][34]. After sacrificing the animals, brain tissues were collected and fixed with 4% (w/v) paraformaldehyde in phosphate-buffered saline (PBS).…”

Section: Immunohistochemical Analysismentioning

confidence: 99%

Behavioral characterization in MPTP/p mouse model of Parkinson’s disease

Wada¹,

Ang²,

Weerasinghe-Mudiyanselage³

et al. 2021

J. Integr. Neurosci.

Self Cite

View full text Add to dashboard Cite

We evaluated the practicability of using the rarely utilized C57BL/6N mouse as a Parkinson's disease model established via the acute MPTP/probenecid (MPTP/p) protocol. We confirmed dopaminergic degeneration in terms of decreased expression levels of tyrosine hydroxylase in the substantia nigra and striatum of MPTP/plesioned mice. In addition, acute MPTP/p-lesioned mice demonstrated initial motor dysfunctions followed by spontaneous recovery. Interestingly, these MPTP/p-lesioned mice exhibited anxiolytic and antidepressive behaviors upon recovery from these motor deficits. Additionally, increased expression of norepinephrine transporters in several brain regions, including the hippocampus, medial prefrontal cortex, and striatum, and an elevated rate of adult neurogenesis (in terms of increased numbers of doublecortin-positive neuroblasts) in the hippocampus were observed a ter recovery from motor dysfunctions. We suggest that the emotional alterations observed under these experimental conditions may be associated with enhanced adult neurogenesis, increased levels of norepinephrine transporters, and/or a possible interplay between these two factors. Consequently, this acute MPTP/p model adequately satisfies the criteria for the validity of a Parkinson's disease model regarding dopaminergic loss and motor impairment. However, the non-motor findings may o fer novel evidence against the practicability of utilizing the acute MPTP/p-lesioned mice for modeling the emotional aberrations found in Parkinson's disease patients.

show abstract

Polyethylene Glycol–Based Polymer-Drug Conjugates: Novel Design and Synthesis Strategies for Enhanced Therapeutic Efficacy and Targeted Drug Delivery

Verma,

Pandey,

Jha

et al. 2024

Appl Biochem Biotechnol

View full text Add to dashboard Cite

Due to their potential to enhance therapeutic results and enable targeted drug administration, polymer-drug conjugates that use polyethylene glycol (PEG) as both the polymer and the linker for drug conjugation have attracted much research. This study seeks to investigate recent developments in the design and synthesis of PEG-based polymer-drug conjugates, emphasising fresh ideas that fill in existing knowledge gaps and satisfy the increasing need for more potent drug delivery methods. Through an extensive review of the existing literature, this study identifies key challenges and proposes innovative strategies for future investigations. The paper presents a comprehensive framework for designing and synthesising PEG-based polymer-drug conjugates, including rational molecular design, linker selection, conjugation methods, and characterisation techniques. To further emphasise the importance and adaptability of PEG-based polymer-drug conjugates, prospective applications are highlighted, including cancer treatment, infectious disorders, and chronic ailments.

show abstract

Melatonin alters neuronal architecture and increases cysteine‐rich protein 1 signaling in the male mouse hippocampus

Cited by 8 publications

References 97 publications

SREBP-1c Deficiency Affects Hippocampal Micromorphometry and Hippocampus-Dependent Memory Ability in Mice

SREBP-1c Deficiency Affects Hippocampal Micromorphometry and Hippocampus-Dependent Memory Ability in Mice

Behavioral characterization in MPTP/p mouse model of Parkinson’s disease

Polyethylene Glycol–Based Polymer-Drug Conjugates: Novel Design and Synthesis Strategies for Enhanced Therapeutic Efficacy and Targeted Drug Delivery

Contact Info

Product

Resources

About