Melatonin and calcium modulate the production of rosmarinic acid, luteolin, and apigenin in Dracocephalum kotschyi under salinity stress

Vafadar, Farinaz; Amooaghaie, Rayhaneh; Ehsanzadeh, Parviz; Ghanadian, Mustafa; Talebi, Majid; Ghanati, Faezeh

doi:10.1016/j.phytochem.2020.112422

Cited by 57 publications

(17 citation statements)

References 40 publications

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“…The total flavonoid content (TFC) of dry extract was calculated using spectrometry method of AlCl3-flavonoid complex as reported before [ 20 ]. Briefly, a 10% diluted solution of dry extract (100 μ L) was added to the test tubes containing 100 μ L of 20% AlCl3 and 20 μ L of glacial acetic acid, and methanol was added to 3 mL.…”

Section: Methodsmentioning

confidence: 99%

“…Flavonoid Content. For standardization of the extract, the content of polyphenols was determined by Folin-Ciocalteu method [20]. For this purpose, 1 g of the extract was transferred to a volumetric flask and reached the volume of 10 ml with methanol.…”

Section: Extract Standardization By Total Phenolic and Totalmentioning

confidence: 99%

“…After preparing different concentrations of gallic acid solution, absorption reading at 765 nm, and drawing the standard curve(y = 0.0551x + 0.0303), total phenol content (TPC) of the extract was calculated as 86.88 ± 0.246 mg gallic acid equivalent per gram of dry extract. e total flavonoid content (TFC) of dry extract was calculated using spectrometry method of AlCl3-flavonoid complex as reported before [20]. Briefly, a 10% diluted solution of dry extract (100 μL) was added to the test tubes containing 100 μL of 20% AlCl3 and 20 μL of glacial acetic acid, and methanol was added to 3 mL.…”

Section: Extract Standardization By Total Phenolic and Totalmentioning

confidence: 99%

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The Effects of Berberis integerrima Fruit Extract on Glycemic Control Parameters in Patients with Type 2 Diabetes Mellitus: A Randomized Controlled Clinical Trial

Soltani

Ghanadian

Iraj

et al. 2021

Evidence-Based Complementary and Alternative Medicine

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Background. Berberis integerrima Bunge fruits have been utilized in traditional medicine to control diabetes mellitus (DM). However, no clinical survey has been done in this regard. This study was conducted to clinically evaluate the effects of fruit extract of this plant in improving glycemic control indices in patients with type 2 DM (T2DM). Methods. In a randomized controlled clinical trial, patients with T2DM who met the inclusion criteria were randomly divided into two groups of drug (Berberis) and control to receive the extract solution 5 ml twice daily (equivalent to 1000 mg of dry extract) with standard treatment (metformin) or only standard treatment, respectively, for 8 weeks. Before and after the intervention, fasting blood sugar (FBS), serum glycosylated hemoglobin (HbA1c), serum insulin, the homeostasis assessment model for insulin resistance (HOMA-IR), body mass index (BMI), and systolic and diastolic blood pressure were determined and compared between the two groups. Results. During the study, 30 and 35 patients in the drug and control groups, respectively, completed the study. Although no significant changes occurred in any parameter within each group, postintervention FBS (117.5 [107–128.8] versus 134 [120–142], P = 0.001 ) and HbA1c (7 [6.4–7.7] versus 7.5 [6.8–7.9], P = 0.045 ) were significantly lower in the drug group than in the control one. In terms of other parameters, there were no significant differences between the groups. Conclusion. Consumption of B. integerrima Bunge fruit extract at a dosage of 1000 mg daily decreases FBS and HbA1c but does not affect HOMA-IR in patients with type 2 diabetes mellitus.

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Section: Methodsmentioning

confidence: 99%

Section: Extract Standardization By Total Phenolic and Totalmentioning

confidence: 99%

Section: Extract Standardization By Total Phenolic and Totalmentioning

confidence: 99%

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The Effects of Berberis integerrima Fruit Extract on Glycemic Control Parameters in Patients with Type 2 Diabetes Mellitus: A Randomized Controlled Clinical Trial

Soltani

Ghanadian

Iraj

et al. 2021

Evidence-Based Complementary and Alternative Medicine

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“…Overall, caffeic, chlorogenic, and rosmarinic acids are the major phenolic compounds of the Mentha genus. Regarding these, 1) rosmarinic acid was found to accumulate in Melissa officinalis L. under heat stress (Pistelli et al, 2019) and in Dracocephalum kotschyi Boiss under saline stress (that also led to luteolin increases) (Vafadar et al, 2020); 2) caffeic acid and its derivatives are actively implicated in different plant defense mechanism toward biotic and abiotic stress agents (Riaz et al, 2019). Generally, the biosynthesis is enhanced under abiotic stress conditions (drought, heavy metal, salinity, high/low temperature, and ultraviolet radiations), resulting in accumulation of various phenols, including caffeic, gallic, and ferulic acids (Sharma et al, 2019).…”

Section: Influence Of Ecological Factors On Mentha Speciesmentioning

confidence: 99%

Impact of Growth Sites on the Phenolic Contents and Antioxidant Activities of Three Algerian Mentha Species (M. pulegium L., M. rotundifolia (L.) Huds., and M. spicata L.)

et al. 2022

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Research studies about the effect of environmental agents on the accumulation of phenolic compounds in medicinal plants are required to establish a set of optimal growth conditions. Hence, in this work, we considered the impact of habitat types, soil composition, climatic factors, and altitude on the content of phenolics in Mentha species [M. pulegium L. (MP), M. rotundifolia (L.) Huds. (MR), and M. spicata L. (MS)] grown in different regions of Algeria. The phenolic contents and antioxidant activities were analyzed using spectrophotometric and HPTLC methods. The harvesting localities differ by their altitudes and climates, but their soils are quite similar, characterized by slight alkalinity, moderate humidity, no-salinity, and high levels in organic matter. Both the contents in total phenolics (TPC), total flavonoids (TFC), and rosmarinic acid (RAC), and the antioxidant activities of Mentha samples collected from these Algerian localities are affected by the geographical regions of origin. The samples of MS and MP from the Khemis–Miliana region showed the highest concentration in TPC (MS, 7853 ± 265 mg GAE/100 g DW; MP, 5250 ± 191 mg GAE/100 g DW), while in Chemini, the MR samples were the richest in these compounds (MR, 3568 ± 195 mg GAE/100 g DW). Otherwise, the MP (from Tichy), MR (from Tajboudjth), and MS (from Khemis–Miliana) specimens exhibited the highest levels of TFC and RAC. The antioxidant levels in a total activity test (reduction of phosphomolybdate) appear correlated with the total phenolic contents, but this was not the case for most of the important ROS-scavenging and iron-chelating capacities for which the quality of polyphenols is probably more important than their amounts. A principal component analysis (PCA) score plot indicates that all of the Mentha samples can be divided into four groups. These discriminated groups appear comparatively similar in phenolic contents and antioxidant activities. As for the harvest localities, the Mentha samples were divided into four groups in which the phenolic contents and antioxidant activities were comparatively equivalent.

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“…Fortunately, in recent years, effective efforts have been made to upregulation of the biosynthetic pathways of this plant and increase the production of its valuable metabolites in the hairy root platform 8 and leaf tissue 9 . However, no research has been conducted on whole transcriptome studies and specific biosynthetic pathways analysis of this to identify the genes involved in the biosynthetic pathways of valuable metabolites (e.g., xanthomicrol, calycopterin, penduletin, and rosmarinic acid) of D. kotschyi .…”

Section: Introductionmentioning

confidence: 99%

Toward understanding of the methoxylated flavonoid biosynthesis pathway in Dracocephalum kotschyi Boiss

Poursalavati

Rashidi-Monfared

Ebrahimi

2021

Sci Rep

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Nowadays, with the development and advancement of next-generation sequencing technologies, a new path has been provided for transcriptomic studies. In this study, the transcriptome of Dracocephalum kotschyi Boiss., as an endemic and endangered plant which is contained a large amount of valuable secondary metabolites with antioxidant and anticancer properties, was sequenced. Then functional annotation and gene ontology analysis for 165,597 assembled transcripts were performed, most were associated with the metabolic pathways. This might be because there are various active biochemical pathways in this plant. Furthermore, after comprehensive transcript annotation, the putative genes involved in the main metabolic pathways of D. kotschyi were identified. Then, the biosynthetic pathway of its valuable methoxylated flavones was proposed. Finally, the accumulations of important methoxylated-flavone metabolites in three different tissues were quantified by HPLC. The relative expression of the genes involved in the proposed pathway was investigated by qRT-PCR, which indicated high expression levels in the bud tissue. The present results may lead to the design strategies to preserve the genetic diversity of endangered D. kotschyi plants and apply the new methods for engineering its valuable methoxylated-flavones pathway.

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Melatonin and calcium modulate the production of rosmarinic acid, luteolin, and apigenin in Dracocephalum kotschyi under salinity stress

Cited by 57 publications

References 40 publications

The Effects of Berberis integerrima Fruit Extract on Glycemic Control Parameters in Patients with Type 2 Diabetes Mellitus: A Randomized Controlled Clinical Trial

The Effects of Berberis integerrima Fruit Extract on Glycemic Control Parameters in Patients with Type 2 Diabetes Mellitus: A Randomized Controlled Clinical Trial

Impact of Growth Sites on the Phenolic Contents and Antioxidant Activities of Three Algerian Mentha Species (M. pulegium L., M. rotundifolia (L.) Huds., and M. spicata L.)

Toward understanding of the methoxylated flavonoid biosynthesis pathway in Dracocephalum kotschyi Boiss

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