Melatonin and CIDR improved the follicular and luteal haemodynamics, uterine and ovarian arteries vascular perfusion, ovarian hormones and nitric oxide in cyclic cows

Abdelnaby, Elshymaa A.; El‐Maaty, Amal M. Abo

doi:10.1111/rda.13888

Cited by 20 publications

(8 citation statements)

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“…We used grey B-mode to detect the numerous testicular anatomical structures and diagnose possible pathologies. Testicular length, width, height and testicular volume (0.523 × height × length × width) were calculated according to the ellipsoid equation (Gouletsou et al, 2008) The ultrasound device used in this current work was EXAGO (Echo Control Medical, France) and was equipped with normal B-mode and modified with colour and pulsed Doppler modes using a linear array probe with a frequency of 5-7.5 MHz (Abdelnaby & Abo El-Maaty, 2021). The Doppler settings are (power: max (100%), pulse repetition frequency: 3.3-5 kHz, Doppler angle: 45°, size of the gate sample: 1 mm, brightness: 70%, two-colour map and maximum Doppler velocity scale is 30 cm/s; EXAGO, France; Fouad et al,2018;Maher, Farghali, Elsayed, et al, 2020).…”

Section: B-mode and Doppler Ultrasonographic Evaluationmentioning

confidence: 99%

Assessment of the accuracy of testicular dysfunction detection in male donkey (Equus asinus) with the aid of colour‐spectral Doppler in relation to plasma testosterone and serum nitric oxide levels

Abdelnaby

Emam

Fadl

2021

Reprod Domestic Animals

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This study aimed to determine the usefulness of colour and pulsed Doppler modes for the accurate diagnosis of donkeys suffering from subfertility to determine whether testicular vascularity assessment could be an indicator for sperm functionality. The study sample was composed of 10 male donkeys with normospermia (control group) and 10 donkeys with hypospermia. Animals underwent scrotal circumference measurement, testicular Doppler examination, seminal evaluation, blood sampling and hormonal assay. Semen volume and concentration were significantly (p ≤ .05) lower in the subfertile group (30.25 ± 1.22 ml and 89.44 ± 2.55 × 106/ml) as compared with the control group (82.76 ± 1.65 ml and 452.78 ± 1.25 × 106/ml), and total sperm/ejaculation was significantly (p ≤ .05) higher in the normal donkeys (28.30 ± 2.32 × 109/total ejaculated) as compared with the subfertile group. Intratesticular coloured area showed a marked decline in the hypospermic males. There was no significant difference between the two groups in testosterone level, although the normal group showed an increase in nitric oxide metabolites. Both Doppler indices of the three branches of the testicular artery were elevated significantly (p ≤ .05) in abnormal donkeys, whereas Doppler peak systolic and end‐diastolic velocities were increased in the normal group. Male donkeys with subfertility demonstrated lower arterial vascularity parameters in the form of intratesticular coloured area and blood flow rate; therefore, the most optimal parameters for differentiating subfertile hypospermic from normospermic donkeys were found to be the two Doppler indices, velocities parameters, testicular blood flow rate and nitric oxide levels.

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Section: B-mode and Doppler Ultrasonographic Evaluationmentioning

confidence: 99%

Assessment of the accuracy of testicular dysfunction detection in male donkey (Equus asinus) with the aid of colour‐spectral Doppler in relation to plasma testosterone and serum nitric oxide levels

Abdelnaby

Emam

Fadl

2021

Reprod Domestic Animals

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“…ER and progesterone receptor (PR) were distinguished in the buffalo ovarian sections [28], both luminal and glandular epithelial cells in addition to stromal cells in the uterus of bovine [28] during the follicular (FP) and luteal (LP) phases and in rabbits during pseudopregnancy [29]. Nitric oxide (NO) is a paracrine mediator with a wide range of physiological roles, including arterial dilation/permeability modulation [30] and neurotransmission [31]. Several lines of evidence imply that NO is involved in cycle-dependent ovarian events, such as ovulation and luteal function modulation [32,33].…”

Section: Introductionmentioning

confidence: 99%

Ovarian, uterine, and luteal vascular perfusions during follicular and luteal phases in the adult cyclic female rabbits with special orientation to their histological detection of hormone receptor

et al. 2022

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Understanding the does reproductive hemodynamic changes during the estrous cycle is crucial for improving reproductive competence and fertility potential in this species. The objective of this study is to investigate the hemodynamic variations in ovarian (OA) and uterine (UA) arteries, histological and morphometric changes in ovarian and uterine tissues throughout the follicular (FP) and luteal (LP) phases in rabbits and determine estrogen (ER), progesterone (PR) receptors, and vascular endothelial growth factor (VEGF) distributions using immunohistochemistry.Fourteen adults pluriparous New Zealand rabbits were divided into rabbits at the FP (Day − 1; n = 7) and those at the LP (Day 9; n = 7). Animals were subjected to Doppler, hormonal (estrogen [E2], progesterone [P4], insulin-like growth factor [ILGF], and VEGF), histological, and immunohistochemical analyses. In LP, OA Doppler indices were significantly increased, whereas peak systolic velocity (PSV) was decreased compared with that in FP. UA Doppler indices were significantly decreased in the LP, whereas PSV was increased (P < 0.05). E2 levels were increased in the FP, whereas P4 levels were increased in the LP. The morphometric analysis of uterine tissues during the LP revealed an increase in the mean uterine endometrium length, endometrial connective tissue area percentage (%), endometrial glands number, myometrial area (%) and thickness. Furthermore, ovarian follicles and corpus luteum (CL) displayed strong positive immunoreactivity for ER, PR, and VEGF-A during both phases. The ovarian sections displayed a substantial (P < 0.05) increase in the area % of VEGF-A in the ovarian follicles during FP while in the CL during LP. Conversely, area percentage of VEGF-A immunoreactivity in the uterine luminal and glandular epithelia during the FP and LP revealed no differences. However, the number of VEGF-A–stained blood capillaries revealed an increase during LP than FP. In conclusion, this study demonstrated for the first time the changes in both ovarian and uterine arteries during two different phases of the rabbit cycle in relation to the histo-morphometric analysis and distribution of ER, PR, and VEGF-A, which regulate uterine functions that play a role in reproduction.

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“…All alterations in uterine vascular perfusion were assessed via a Doppler ExaGo device (made in France) with a 5–7.5 MHz linear probe. The uterus was examined with a slow scan of the entire uterus by B‐mode and then colour mode was activated to capture a 15–30 s video for later recording (Abdelnaby & Abo El‐Maaty, 2021). The evaluation protocol includes the presence of pixels of the uterine body (using Image J program) represented by the blue and red colours of the colour Doppler, and the vascular distribution of the uterus, including all layers (Abdelnaby, El‐Maaty, & El‐Badry, 2021).…”

Section: Methodsmentioning

confidence: 99%

The therapeutic effect of stem cell‐derived exosomes in the treatment of chronic endometritis as assessed by histopathological, Doppler and hormonal expression in Arabian mares

Abdelnaby,

Abdallah,

Anwar

et al. 2023

Equine Veterinary Education

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SummaryBackgroundBone‐derived exosomes are considered nano‐sized extracellular vesicles secreted by the bone marrow stromal cells.ObjectivesThis study used exosomes derived from bone marrow mesenchymal stem cells to treat chronic endometritis in Arabian mares with follow‐up alterations in haemodynamics, hormonal and histopathological expressions.Methods and study designFourteen (n = 14) Arabian mares (aged 13–15 years old) were equally divided into the control group and an endometritis group (chronic type). Exosomes were infused twice 21 days apart at the first and second ovulations. Follow‐up was done ultrasonographically, and samples were taken for histopathology. Blood samples were taken to estimate hormonal levels.ResultsUterine biopsy samples before treatment showed a marked degree of degeneration, while the second exosomal treatment showed regression of fibrous tissue and restoration of healthy endometrial glands with normal epithelium. Uterine body and horns thickness/mm were decreased after treatment at day 0 of the 3rd ovulation compared to day −1 before treatment, the uterine body‐coloured area and total uterine coloured areas/pixels were elevated (p < 0.05) at day 0 of the 3rd ovulation compared to day −1 before treatment. Uterine artery blood parameters (pulsatility index, resistance index, peak systolic and end‐diastolic points) did not change before, during and after treatment, but the blood flow rate was elevated (p < 0.05) on day 0 of the 3rd ovulation compared to day −1 before treatment. Oestradiol level was decreased (p < 0.05), while progesterone level was increased (p < 0.05) on day 0 of the 3rd ovulation compared to day −1 before treatment.ConclusionTwo intrauterine administrations of exosomes restored the normal endometrial appearance after chronic endometritis resulting in the elevation of the conception rate in infertile mares.

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Melatonin and CIDR improved the follicular and luteal haemodynamics, uterine and ovarian arteries vascular perfusion, ovarian hormones and nitric oxide in cyclic cows

Cited by 20 publications

References 46 publications

Assessment of the accuracy of testicular dysfunction detection in male donkey (Equus asinus) with the aid of colour‐spectral Doppler in relation to plasma testosterone and serum nitric oxide levels

Assessment of the accuracy of testicular dysfunction detection in male donkey (Equus asinus) with the aid of colour‐spectral Doppler in relation to plasma testosterone and serum nitric oxide levels

Ovarian, uterine, and luteal vascular perfusions during follicular and luteal phases in the adult cyclic female rabbits with special orientation to their histological detection of hormone receptor

The therapeutic effect of stem cell‐derived exosomes in the treatment of chronic endometritis as assessed by histopathological, Doppler and hormonal expression in Arabian mares

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