Melatonin induces apoptosis and cell cycle arrest in cervical cancer cells via inhibition of NF-κB pathway

Minocha, Tarun; Das, Megha; Rai, Vipin; Verma, Sumit Singh; Awasthee, Nikee; Gupta, Subash C.; Haldar, Chandana; Yadav, Sanjeev Kumar

doi:10.1007/s10787-022-00964-6

Cited by 20 publications

(7 citation statements)

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“…To study the cytotoxicity of the extracts selected on the cell survival, the HepG2 cells were incubated using different concentrations of herbal extracts (1 µg, 10 µg, 50 µg, 100 µg, 200 µg, 500 µg, and 1000 µg) of all three plants. The cytotoxicity of Capsella bursa-pastoris , Inula racemosa, and Urtica dioica was assessed in the liver cancer HepG2 cell line, as per the method described earlier [ 64 ]. The HepG2 cells were grown-up in 75 cm 2 flasks in DMEM (Gibco Dulbecco’s Modified Eagle Medium), augmented with 100 U/mL penicillin, 5% fetal bovine serum (FBS), and 100 mg/mL streptomycin under standard conditions (humid environment, 5% CO 2 and 37 °C) and the cells were sub-cultured after every 4 days, and the growth media was renewed after every 2 days.…”

Section: Methodsmentioning

confidence: 99%

Appraisal of the Antioxidant Activity, Polyphenolic Content, and Characterization of Selected Himalayan Herbs: Anti-Proliferative Potential in HepG2 Cells

Yousuf

Shabir²,

Kauts³

et al. 2022

Molecules

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Natural antioxidants derived from plants have played a vital role in preventing a wide range of human chronic conditions and provide novel bioactive leads for investigators in pharmacotherapy discovery. This work was designed to examine the ethnopharmacological role of Urtica dioica (UD), Capsella bursa-pastoris (CBP), and Inula racemosa (IR). The total phenolic and flavonoid contents (TPC and TFC) were illustrated through colorimetric assays, while the antioxidant activity was investigated through DPPH and ABTS assays. The evaluation of phytochemicals by FT-IR of UD and CBP revealed high contents of aliphatic amines, while IR showed a major peak for ketones. The antioxidant activity, TPC and TFC were highest in the ethanol extract of UD, followed by CBP, and IR showed the lowest activity. All of the extracts revealed significant antioxidant capacities along a dosage gradient. Through a HPLC analysis at a wavelength of 280 nm, UD leaves demonstrated an intense peak of quercetin, and the peak for rutin was less intense. CBP (whole plant), instead, demonstrated a major yield of rutin, and a peak for quercetin was not observed in CBP. IR (rhizomes) showed both quercetin and rutin. All of the extracts were significantly cytotoxic to HepG2 cells after 48 h with the trend IR > UD > CBP. The outcomes of this study may be effective in the selection of specific plants as realistic sources of the bioactive components that might be useful in the nutraceutical progression and other biomedical efficacies.

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Section: Methodsmentioning

confidence: 99%

Appraisal of the Antioxidant Activity, Polyphenolic Content, and Characterization of Selected Himalayan Herbs: Anti-Proliferative Potential in HepG2 Cells

Yousuf

Shabir²,

Kauts³

et al. 2022

Molecules

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“…6,8,9,11 G5 was significantly different from G6 and G7 (p < 0.05). 7,10 G6 was significantly different from G7 and G8 (p < 0.05).…”

Section: Oxidative Stressmentioning

confidence: 80%

“…Furthermore, the application of MEL to lemons has been found to reduce the incidence of fungal and bacterial diseases in the fruit, maintaining its nutritional quality and prolonging shelf life [7][8][9]. Also, melatonin has been shown to have antioxidant and anti-inflammatory effects on human health that may help prevent cancer and other HPV-induced diseases [10]. In addition, exposure of SARS-CoV-infected cells to melatonin has been shown to inhibit the growth and proliferation of the virus [11].…”

Section: Introductionmentioning

confidence: 99%

The Effect of Lemon Juice (Citrus limon L.) Treated with Melatonin on the Health Status and Treatment of K14HPV16 Mice

Badiche-El Hilali,

Medeiros-Fonseca,

Silva

et al. 2024

Antioxidants

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Lemon is a fruit rich in antioxidant properties and has several health benefits, namely the reduction of skin edema and anticarcinogenic properties, which are due to its high content of bioactive compounds. Melatonin can improve and preserve the properties of lemon for longer and also has health benefits. The aim of this study was to evaluate the effects of oral administration of lemon juice after melatonin treatment on murinometric parameters of wild-type (WT) mice and transgenic mice carrying human papillomavirus (HPV). Two trials were performed for oral administration of the lemon extract compound: in drinking water and in diet. First of all, lemons were treated by immersion with melatonin at 10 mM. Then, lemons were squeezed, and the juice obtained was freeze-dried and stored to be subsequently added to drinking water or diet, according to the assay. Thus, mice were divided into eight groups in the drink assay (each with n = 5): group 1 (G1, WT, control), group 2 (G2, WT, 1 mL lemon), group 3 (G3, WT, 1.5 mL lemon), group 4 (G4, WT, 2 mL lemon), group 5 (G5, HPV16, control), group 6 (G6, HPV16, 1 mL lemon) group 7 (G6, HPV16, 1.5 mL lemon) and group 8 (G6, HPV16, 2 mL lemon). The diet assay was divided into four groups: group 1 (G1, WT, control), group 2 (G2, WT, 4 mL lemon), group 3 (G3, HPV16, control) and group 4 (G4, HPV16, 4 mL lemon). In the drink assay, the highest concentration of melatonin (308 ng/100 mL) was for groups 4 and 8, while in the food assay, there was only one concentration of melatonin (9.96 ng/g) for groups 2 and 4. Both trials lasted 30 days. During this time, body weight, food and water were recorded. Afterward, they were sacrificed, and samples were collected for different analyses. At the concentrations used, the lemon juice with melatonin had no adverse effects on the animals’ health and showed a positive outcome in modifying weight gain and enhancing antioxidant activity in mice. Moreover, a reduction in the incidence of histological lesions was observed in treated animals. Further research is needed to better understand the effects of lemon extract on health and treatment outcomes in this animal model.

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“…The cell viability assay was carried out to speculate the cytotoxicity of BBR and MSNs–BBR in the neuroblastoma cell line. 27,28 SH-SY-5Y cells were plated in a 96-well plate and treated with different concentrations of BBR and MSNs–BBR for 24 h. Thereafter, the cells were incubated with MTT (10 μL) for 2 h and subjected to DMSO (100 μL) in each well. Finally, the absorbance was recorded at 570 nm using a microplate reader.…”

Section: Methodsmentioning

confidence: 99%

In vitro profiling and molecular dynamics simulation studies of berberine loaded MCM-41 mesoporous silica nanoparticles to prevent neuronal apoptosis

Singh,

Minocha

et al. 2024

Nanoscale Adv.

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Melatonin induces apoptosis and cell cycle arrest in cervical cancer cells via inhibition of NF-κB pathway

Cited by 20 publications

References 50 publications

Appraisal of the Antioxidant Activity, Polyphenolic Content, and Characterization of Selected Himalayan Herbs: Anti-Proliferative Potential in HepG2 Cells

Appraisal of the Antioxidant Activity, Polyphenolic Content, and Characterization of Selected Himalayan Herbs: Anti-Proliferative Potential in HepG2 Cells

The Effect of Lemon Juice (Citrus limon L.) Treated with Melatonin on the Health Status and Treatment of K14HPV16 Mice

In vitro profiling and molecular dynamics simulation studies of berberine loaded MCM-41 mesoporous silica nanoparticles to prevent neuronal apoptosis

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