Melatonin inhibits nuclear factor kappa B activation and oxidative stress and protects against thioacetamide induced liver damage in rats

Bruck, Rafael; Aeed, Hussein; Avni, Yona; Shirin, Haim; Matas, Zipora; Shahmurov, Mark; Avinoach, Ilana; Zozulya, G.; Weizman, Noam; Hochman, Ayala

doi:10.1016/s0168-8278(03)00504-x

Cited by 163 publications

(121 citation statements)

References 49 publications

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“…Previous studies revealed that inhibition of NF-jB activity can improve TAAinduced acute liver damage in rats (Bruck et al 2002(Bruck et al , 2004Shapiro et al 2006). Administration of the iNOS inhibitor aminoguanidine also reduces the severity of damage in the liver and improves mortality after TAA intraperitoneal administration (Rahman & Hodgson 2003).…”

Section: Discussionmentioning

confidence: 98%

Single dose intravenous thioacetamide administration as a model of acute liver damage in rats

Chen

Subeq

Lee

et al. 2008

Int J Experimental Path

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1Contributed equally to this study. SummaryThioacetamide (TAA) has been used extensively in the development of animal models of acute liver injury. Frequently, TAA is administered intraperitoneally to induce liver damage under anaesthesia. However, it is rarely administered by intravenous injection in conscious rats. The experiments in this study were designed to induce acute liver damage by single intravenous injection of TAA (0, 70 and 280 mg ⁄ kg) in unrestrained rats. Biochemical parameters and cytokines measured during the 60-h period following TAA administration, included white blood cells (WBC), haemoglobulin (Hb), platelet, aspartate transferase (GOT), alanine transferase (GPT), total bilirubin (TBIL), direct bilirubin (DBI), albumin, ammonia (NH3), r-glutamyl transpeptidase (r-GT), tumour necrosis factor-a (TNF-a) and interleukin-6 (IL-6). Rats were sacrificed by decapitation 60 h after TAA administration and livers were removed immediately for pathology and immunohistochemical (IHC) examination. Another group of rats were sacrificed by decapitation 1, 6 and 24 h after TAA administration and livers were removed immediately for time course change of pathology and IHC examination. TAA significantly increased blood WBC, GOT, GPT, TBIL, DBIL, NH3, r-GT, TNF-a and IL-6 levels but decreased the blood Hb, platelet and albumin level. The levels of histopathological damage in the liver after intravenous TAA administration were also increased with a dose-dependent trend and more increased at 60 h after TAA administration. The levels of inducible nitric oxide synthase (iNOS) and nuclear factor-jB (NF-jB) detected by IHC in the liver after intravenous TAA administration were also increased with a dose-dependent trend and more increased at 1 h after TAA administration. Single intravenous TAA administration without anaesthesia is a restorable animal model which may be used to investigate acute liver damage.

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Section: Discussionmentioning

confidence: 98%

Single dose intravenous thioacetamide administration as a model of acute liver damage in rats

Chen

Subeq

Lee

et al. 2008

Int J Experimental Path

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“…Melatonin reduces nf-κB binding in spleen [89]. a number of laboratories report melatonin-induced inhibition of nK-κB activity or inhibition of nf-κB activation [90][91][92][93][94][95][96][97][98][99][100]. these results suggest an interaction of melatonin and the ubiquitin-proteasome system in the activation of nf-κB.…”

Section: Melatonin/ubiquitin Interaction In Brown Fatmentioning

confidence: 92%

Melatonin and ubiquitin: what’s the connection?

Vriend

Reíter

2014

Cell. Mol. Life Sci.

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“…Liver failure was induced by intraperitoneal (ip) injections of 300 mg/kg TAA (Merck, Darmstadt, Germany) daily for 2 days (11,12). Subcutaneous 5% dextrose (25 mL/kg) and 0.9% NaCl with potassium (20 mEq/L) were given every 6 h as supportive therapy to prevent weight loss, hypoglycemia, and renal failure as described previously (5).…”

Section: Induction Of Liver Failurementioning

confidence: 99%

Methylprednisolone prevents bacterial translocation in thioacetamide-induced liver failure in rats

Harputluoğlu

Temel²,

Demirel³

et al. 2017

Turk J Gastroenterol

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Melatonin inhibits nuclear factor kappa B activation and oxidative stress and protects against thioacetamide induced liver damage in rats

Cited by 163 publications

References 49 publications

Single dose intravenous thioacetamide administration as a model of acute liver damage in rats

Single dose intravenous thioacetamide administration as a model of acute liver damage in rats

Melatonin and ubiquitin: what’s the connection?

Methylprednisolone prevents bacterial translocation in thioacetamide-induced liver failure in rats

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