Melatonin-mediated effects on killifish reproductive axis

Lombardo, Francesco; Gioacchini, Giorgia; Fabbrocini, Adele; Candelma, Michela; D’Adamo, Raffaele; Giorgini, Elisabetta; Carnevali, Oliana

doi:10.1016/j.cbpa.2014.02.008

Cited by 27 publications

(17 citation statements)

References 40 publications

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“…While ovulation was found to occur within 12 h of ovaprim treatment, no signs of egg release were detected until 16 h after intramuscular injection of a single dose of melatonin, indicating that exogenous melatonin per se did not have any significant effects on the process of ovulation. Apparently, the findings on carp are not consistent with the reports on zebrafish (Carnevali et al 2011) and killifish (Lombardo et al 2014), where melatonin administration through water for 10 or 8 days caused an increase in the number of spawned eggs. However, it is notable that the dose, route and duration of melatonin treatment in the present and earlier studies were not identical.…”

Section: Discussioncontrasting

confidence: 93%

“…The likely scenario is that 2-h melatonin priming may be required for sensitizing signaling pathways for sGnRH and domperidone in the HP axis to enhance the effects of ovaprim. Thus, it is likely that ovaprim, as the substituted GnRH, acts on the hypothalamo-hypophyseal system (Williot et al 2002), while melatonin appears to work at both the central endocrine system and the ovary at the downstream of the reproductive axis to accelerate the process of oocyte maturation , Lombardo et al 2014. However, the mechanism of actions of the hormones/ drugs administered on the HPG axis, especially on the ovary, remains speculative and thereby warrants further study.…”

Section: Discussionmentioning

confidence: 99%

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Melatonin actions on ovaprim (synthetic GnRH and domperidone)-induced oocyte maturation in carp

Moniruzzaman¹,

Hasan²,

Maitra³

2016

Reproduction

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The major objective of the present study was to demonstrate the actions of exogenous melatonin on ovaprim (synthetic GnRH and domperidone)-induced final oocyte maturation focusing on the oxidative status of pre-ovulatory follicles in the carp Catla catla. Accordingly, gravid carp during the early spawning phase of the reproductive cycle were injected with melatonin and/or ovaprim at different time intervals or luzindole (a pharmacological blocker of melatonin receptors) before their administration. We studied their effects on the latency period, the rate of germinal vesicle breakdown (GVBD; a visual marker of final oocyte maturation) in oocytes, and the levels of maturation-promoting factor (MPF), as well as oxidative stress, different antioxidants, melatonin and MT1 melatonin receptor protein in the extracts of pre-ovulatory follicles. Notably, melatonin treatment 2 h before the injection of ovaprim resulted in the shortest latency period as well as the highest rate of GVBD and MPF formation. Exogenous melatonin, irrespective of the injection schedule, caused a significant reduction in intra-follicular oxidative stress and an increase in the levels of both enzymatic and nonenzymatic antioxidants, melatonin and its receptor protein. Concentrations of ovarian melatonin in each fish exhibited a significant negative correlation with the level of oxidative stress, but a positive correlation with the rate of GVBD and the activity/level of different antioxidants. However, no significant effects of melatonin and/or ovaprim were detected in luzindole-pretreated carp. Collectively, the present study provides the first evidence that melatonin pretreatment in carp ameliorates ovaprim actions on the process of final oocyte maturation by the formation of MPF and alleviates oxidative stress in pre-ovulatory follicles by stimulating different antioxidants.

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Section: Discussioncontrasting

confidence: 93%

Section: Discussionmentioning

confidence: 99%

Melatonin actions on ovaprim (synthetic GnRH and domperidone)-induced oocyte maturation in carp

Moniruzzaman¹,

Hasan²,

Maitra³

2016

Reproduction

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“…Lombardo et al. () exposed killifish to melatonin‐supplemented rearing water and observed a greater portion of motile sperm with significantly elevated VCL. They suggested that the melatonin acts as an antioxidant and thus may enhance motility (Lombardo et al., ).…”

Section: Discussionmentioning

confidence: 99%

“…() exposed killifish to melatonin‐supplemented rearing water and observed a greater portion of motile sperm with significantly elevated VCL. They suggested that the melatonin acts as an antioxidant and thus may enhance motility (Lombardo et al., ). Indeed, oxidative stress can have detrimental consequences for spermatozoa, such as DNA damage (Pérez‐Cerezales et al., ) or lipid peroxidation (Shaliutina, Hulak, Gazo, Linhartova, & Linhart, ).…”

Section: Discussionmentioning

confidence: 99%

“…There is evidence that melatonin can be beneficial to enhance or conserve sperm quality (motility and/or velocity), as observed in vivo in killifish Fundulus heteroclitus (Lombardo et al, 2014), in ram (Ashrafi, Kohram, Naijian, Bahreini, & Poorhamdollah, 2011) and post-thawed bull semen (Ashrafi, Kohram, & Ardabili, 2013), which was associated with a reduction of oxidative stress such as lipid peroxidation, as well as an increase in the total antioxidant capacity and antioxidant enzyme activity. Similarly, melatonin prevents sperm apoptosis in humans after ejaculation (Espino et al, 2010), which is already applied experimentally in assisted reproduction (Bejarano et al, 2014).…”

Section: Introductionmentioning

confidence: 98%

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Management of pikeperchSander lucioperca(Linnaeus, 1758) sperm quality after stripping

Schaefer

Overton²,

Bossuyt

et al. 2016

J. Appl. Ichthyol.

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Summary The aim of this study was to identify the potential for optimizing management of sperm quality during commercial reproduction of pikeperch Sander lucioperca. Sperm from different males is often pooled prior to fertilization or stored for short periods (hr) until ovulated eggs become available. A novel approach was applied to assess pooling effects by cross‐wise transfusion of sperm and seminal fluid (SF) of males with differing initial sperm quality. In addition, the effects of two different buffers (glucose and KCl) were tested, as well as a supplementation of melatonin and progesterone (1 mmol L−1) to maintain or improve the quality of freshly stripped and incubated (0.5, 1, 2, 4 and 24 hr) sperm. Sperm motility and curvilinear velocity (VCL) were measured by computer assisted sperm analysis (CASA). The VCL proved to be a more sensitive, reliable parameter compared to motility, since significant differences occurred up to 3.5 hr earlier. Transfusion of SF between low and high quality sperm resulted in a significant decrease in sperm with high initial VCL (seven out of 22 transfusions), whereas VCL of low quality sperm could not be improved. In only one case did a transfusion result in an increased VCL. No treatment prevented a significant quality loss over 24 hr or even enhanced sperm performance. Conclusively, pooling sperm of different qualities as well as short‐term storage has a significant, negative impact on overall sperm quality. Pooling should only be considered when the sperm quality is known.

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Melatonin and Seasonal Reproduction in Teleosts

Hafeez

Ahmad

2021

Recent Updates in Molecular Endocrinology and Reproductive Physiology of Fish

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Melatonin-mediated effects on killifish reproductive axis

Cited by 27 publications

References 40 publications

Melatonin actions on ovaprim (synthetic GnRH and domperidone)-induced oocyte maturation in carp

Melatonin actions on ovaprim (synthetic GnRH and domperidone)-induced oocyte maturation in carp

Management of pikeperchSander lucioperca(Linnaeus, 1758) sperm quality after stripping

Melatonin and Seasonal Reproduction in Teleosts

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