Melatonin modifies the expression of the genes for nuclear- and plastid-encoded chloroplast proteins in detached Arabidopsis leaves exposed to photooxidative stress

ROS and NO Regulation by Melatonin Under Abiotic Stress in Plants

“…Perhaps one of the most important functions of MET in plants is the improvement of the photosynthetic efficiency under most abiotic stresses such as salinity [48,107,108,135,136], high temperature [137], cold [36,54,95], drought [33,98,113,118], high light [102,138], waterlogging [119,122,139,140], S deficiency [141] and stress combination [10]. verified that MET improves the photosynthetic activities of tomato (Solanum lycopersicum cv.…”

“…Moreover, the analyses using metabolomics demonstrated that drought-stressed plants treated with MET showed differentially regulated key metabolic pathways such as the phenylpropanoid, Chl and carotenoid biosynthesis pathways, carbon fixation, and sugar metabolism [33]. Moreover, exogenous MET reduced the negative effect of excess light by increasing the efficiency of the photosystems and rearranging the expression of chloroplast-and nuclear-encoded genes (housekeeping genes involved in the maintenance of transcriptional activity and the functional state of chloroplasts) in detached A. thaliana leaves [138].…”

The melatonin receptor CAND2 is involved in the regulation of photosynthesis and chloroplast gene expression in Arabidopsis thaliana under photooxidative stress

“…Protein isolation and Western blot analysis: Protein extraction and Western blot analysis were performed according to our previous study (Bychkov et al 2019). In brief, the samples were blotted to PVDF membrane after SDS-PAGE electrophoresis and blots were incubated with anti-ELIP1 (Heddad et al 2006), anti-PsbD (PSII; AS06 146), anti-RbcL (Rubisco; AS03 037), anti-AtpB (ATP synthase; AS05 085), and anti-PsaB (PSI; AS10 695) primary antibodies (Agrisera, Sweden) overnight at 4°C followed by the secondary antibody (anti-rabbit IgG horseradish peroxidases conjugated from Agrisera, AS09 602) for 1 h at room temperature according to the manufacturer's instructions.…”

“…The activity of chloroplast genes, at least in part, may be maintained due to the elevated expression of genes for chloroplast RNA polymerases. Previously, we showed that under mild photooxidative stress melatonin induces the expression of genes for almost all elements of the chloroplast transcription machinery, including NEP, PEP, sigma factors, and PEP-associated proteins in detached Arabidopsis leaves (Bychkov et al 2019). In the described experimental design, melatonin rather maintained the activity of PEP and RPOTp under increased photodamage, although in the highly sensitive asmt mutant, it even induced RPOTp expression in stress conditions.…”

“…In previous work, we showed that exogenous melatonin increases the expression of the genes encoding chloroplast transcription machinery under mild photooxidative stress in Arabidopsis detached leaves (Bychkov et al 2019). This work is aimed to elucidate whether exogenous melatonin regulates chloroplast gene expression and photosynthesis in young intact Arabidopsis plants under severe HL stress and to unravel how melatonin deficiency and disruption of its putative signaling pathway affect molecular processes in chloroplasts.…”