Melatonin protects against acrylamideinduced oxidative tissue damage in rats

Abstract

INTRODUCTION Acrylamide (CH 2 =CHCONH 2 ) is an , -unsaturated carbonyl compound with a significantly high chemical activity (1). It is extensively used mainly in the manufacture of water-soluble polymers. These polymers are primarily employed by the wastewater, paper, mining, and oil industry (2). ACR does not occur naturally. It was found in various fried, deep-fried and oven-baked foods. It occurs in foods that are regularly consumed throughout the years, not only crisps and bread, but also biscuits, crackers and breakfast cereals. Recent studies demonstrates that acrylamide can also be formed at physiological conditions (37 degrees C, pH 7.4) when asparagine is incubated in the presence of hydrogen peroxide (H 2 O 2 ). Nevertheless, it is presumably not physiologically produced in toxic concentrations (1,3).Acrylamide is toxic and an irrritant. Cases of acrylamide poisoning show signs and symptoms of local effects due to irritation of the skin and mucous membranes and systemic effects due to the involvement of the central, peripheral, and autonomic nervous systems. Studies of neurotransmitter distribution and receptor binding in the brain of rats have revealed changes induced by acrylamide. In rats, changes in the concentration of neurotransmitters and in striatal dopamine receptor binding have been related to behavioural changes. Degenerative changes in renal convoluted tubular epithelium and glomeruli and fatty degeneration and necrosis of the liver have been seen in monkeys given large doses of acrylamide. In rats, impairment of hepatic porphyrin metabolism has been observed. The toxicity of ACR is at least in part related to free radicals and free radical-mediated oxidative stress. Thus, it would be of therapeutic benefit to develop new drugs that are capable of scavenging these free radicals in the treatment of acrylamide-induced damage (4). After decapitation, liver and kidney tissues were excised. Malondialdehyde (MDA), glutathione (GSH) levels, collagen contents and myeloperoxidase activity (MPO) were determined in the tissues, while enzyme activities and cytokine levels were assayed in blood samples. In the ACR treated group, GSH levels decreased significantly while the MDA levels, MPO activity and collagen content increased in the tissues suggesting oxidative organ damage. In the MEL treated ACR group, all of these oxidant responses were reversed significantly. Serum enzyme activities, cytokine levels and leukocyte apoptosis which increased significantly following ACR administration, decreased with MEL treatment. The results demonstrate the role of oxidative mechanisms in ACR-induced tissue damage, and melatonin, by its antioxidant properties, ameliorates oxidative organ injury due to acrylamide toxicity. ABSTRACT: Acrylamide (ACR), is a widely used industrial chemical which induces

Assessment the Protective Role of Quercetin on Acrylamide-Induced Oxidative Stress in Rats

“…TA transversions. Our results showed that 8-OHdG level in the ACR-treated group significantly increased compared with the control group, which is in agreement with the findings of Tozan-Beceren et al (2012). In the present study, increases in MDA, PC and 8-OHdG levels induced by ACR were prevented by quercetin treatment demonstrating that ACR caused oxidative organ injury in the liver, brain, testes and kidney tissues were Values are expressed as means 6 SEM (n 5 10).…”

“…Glutathione is a cysteine-containing tripeptide that plays a critical role in the protection of tissues by directly interacting with ROS as well as an important component in the metabolism of many xenobiotics, like ACR (Kurebayashi and Ohno, 2006;Siu et al 2013). It has been demonstrated that ACR caused depletion of GSH content (Tozan-Beceren et al 2012), which is in agreement with our results. Quercetin has been found to increase the expression of c-glutamylcysteine synthetase with a concomitant increase in the intracellular glutathione concentrations (Moskaug et al 2005).…”

The protective role of pycnogenol against oxidative stress induced by feeding young male rats on potato chips

“…Disturbances in the normal redox state by ACY can cause toxic effects through the production of cell damaging peroxides and free radicals. A decline in serum TAC coincided with the results obtained by Tozan-Beceren et al (2012).…”

Effects of Inositol Supplementation in Sperm Extender on the Quality of Cryopreserved Mesopotamian Catfish (Silurus triostegus, H. 1843) Sperm

“…The supposition that supplementation with a high dose of inositol to the extender decreased DNA damage was assessed by using 8-OHdG after freezing and thawing. Previous study results showed that acrylamide treatment resulted in a high plasma level of 8-OHdG, which was decreased when rats were treated with melatonin [ 76 ]. Similarly, Governini et al [ 54 ] measured the level of 8-OHdG in treated and untreated sperm with MI (20 mg/mL) to evaluate the ability of MI to prevent DNA damage.…”