Melatonin protects against defects induced by deoxynivalenol during mouse oocyte maturation

Lan, Mei; Han, Jun; Pan, Meng-Hao; Wan, Xiang; Pan, Zhen‐Nan; Sun, Shao‐Chen

doi:10.1111/jpi.12477

Cited by 88 publications

(70 citation statements)

References 40 publications

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“…Melatonin has important physiological functions, such as the control of circadian rhythms, decrease in oral temperature, regulation of follicular development and reproductive activity, and activation of the immune system . Previous research reports found that melatonin exerts a protective function in mouse oocytes and porcine oocytes . Our results agree with previous studies which suggest that melatonin plays an important role in steroid hormone synthesis of GCs, however, the mechanism underlying how melatonin affects steroid hormone synthesis in GCs is unclear.…”

Section: Discussionsupporting

confidence: 90%

Effects of melatonin on the synthesis of estradiol and gene expression in pig granulosa cells

Liu

Yang

et al. 2019

Journal of Pineal Research

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The interaction of granulosa cells (GCs) with oocytes is important to regulate follicle development. The exogenous melatonin promoting the maturation of oocytes by GCs has been approved in pig, however, the transcriptome profile and the functions of the genes regulated by melatonin in GCs have not yet to be fully characterized. In this study, we found melatonin could stimulate the synthesis of estradiol in pig GCs. The RNA‐seq was used to explore the effects of melatonin on gene expression, a total of 89 differentially expressed genes (DEGs) were identified. Gene ontology analysis showed DEGs which associated with regulation of cell proliferation, cell cycle, and anti‐apoptosis were significantly enriched. The functions of two DEGs, NOTCH2 and FILIP1L, were studied in pig GCs. The results showed that NOTCH2 inhibited the synthesis of estradiol, but FILIP1L promoted the synthesis of estradiol. Furthermore, inhibiting NOTCH2 in granulosa cells cocultured with cumulus‐oocyte‐complexes had no obvious effect on the maturation of pig oocyte, but could upregulate the cleavage rate of oocyte. We proved that FILIP1L had no effect on the maturation and cleavage of pig oocytes. Our work deepens the understanding of melatonin's effects on GCs and oocyte. The DEGs we found will be beneficial to reveal mechanisms of melatonin acting on GCs and oocytes and design the pharmacological interventions.

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Section: Discussionsupporting

confidence: 90%

Effects of melatonin on the synthesis of estradiol and gene expression in pig granulosa cells

Liu

Yang

et al. 2019

Journal of Pineal Research

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“…In the present study, melatonin increased the global H3K9ac levels, reduced the H3K9me3 levels, and did not affected DNA methylation and hydroxymethylation in oocytes. Lan et al (2018) and Y. Zhang et al (2018) also proved that melatonin could restore abnormal epigenetic alterations induced by deoxynivalenol and MEHP in mouse and porcine oocytes.…”

Section: Discussionmentioning

confidence: 83%

Melatonin supplementation during in vitro maturation of oocyte enhances subsequent development of bovine cloned embryos

Peng

Cheng

et al. 2019

Journal Cellular Physiology

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Oocyte quality, which is directly related to reprogramming competence, is a major important limiting factor in animal cloning efficiency. Compared with oocytes matured in vivo, in vitro matured oocytes exhibit lower oocyte quality and reprogramming competence primarily because of their higher levels of reactive oxygen species. In this study, we investigate whether supplementing the oocyte maturation medium with melatonin, a free radical scavenger, could improve oocyte quality and reprogramming competence. We found that 10−9 M melatonin effectively alleviated oxidative stress, markedly decreased early apoptosis levels, recovered the integrity of mitochondria, ameliorated the spindle assembly and chromosome alignment in oocytes, and significantly promoted subsequent cloned embryo development in vitro. We also analyzed the effects of melatonin on epigenetic modifications in bovine oocytes. Melatonin increased the global H3K9 acetylation levels, reduced the H3K9 methylation levels, and minimally affected DNA methylation and hydroxymethylation. Genome‐wide expression analysis of genes in melatonin‐treated and nontreated oocytes was also conducted by high‐throughput RNA sequencing. Our results indicated that melatonin ameliorates oocyte oxidative stress and improves subsequent in vitro development of bovine cloned embryos.

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“…A total of 2 μM DON was shown to affect the formation of the meiotic spindle in mouse oocytes ( Lan et al, 2018 ). While a recent study showed that 10 μM DON affected the morphology of pig ovaries with an ex vivo approach ( Gerez, Desto & Bracarense, 2017 ).…”

Section: Discussionmentioning

confidence: 99%

Comparison of the toxic effects of different mycotoxins on porcine and mouse oocyte meiosis

Zhang

Liu

et al. 2018

PeerJ

Self Cite

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BackgroundAflatoxin B1 (AFB1), deoxynivalenol (DON), HT-2, ochratoxin A (OTA), zearalenone (ZEA) are the most common mycotoxins that are found in corn-based animal feed which have multiple toxic effects on animals and humans. Previous studies reported that these mycotoxins impaired mammalian oocyte quality. However, the effective concentrations of mycotoxins to animal oocytes were different.MethodsIn this study we aimed to compare the sensitivity of mouse and porcine oocytes to AFB1, DON, HT-2, OTA, and ZEA for mycotoxin research. We adopted the polar body extrusion rate of mouse and porcine oocyte as the standard for the effects of mycotoxins on oocyte maturation.Results and DiscussionOur results showed that 10 μM AFB1 and 1 μM DON significantly affected porcine oocyte maturation compared with 50 μM AFB1 and 2 μM DON on mouse oocytes. However, 10 nM HT-2 significantly affected mouse oocyte maturation compared with 50 nM HT-2 on porcine oocytes. Moreover, 5 μM OTA and 10 μM ZEA significantly affected porcine oocyte maturation compared with 300 μM OTA and 50 μM ZEA on mouse oocytes. In summary, our results showed that porcine oocytes were more sensitive to AFB1, DON, OTA, and ZEA than mouse oocytes except HT-2 toxin.

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Melatonin protects against defects induced by deoxynivalenol during mouse oocyte maturation

Cited by 88 publications

References 40 publications

Effects of melatonin on the synthesis of estradiol and gene expression in pig granulosa cells

Effects of melatonin on the synthesis of estradiol and gene expression in pig granulosa cells

Melatonin supplementation during in vitro maturation of oocyte enhances subsequent development of bovine cloned embryos

Comparison of the toxic effects of different mycotoxins on porcine and mouse oocyte meiosis

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