Melittin – A bee venom component – Enhances muscle regeneration factors expression in a mouse model of skeletal muscle contusion

Lee, Jae Eun; Shah, Vikash Kumar; Lee, Eunjung; Oh, Minseok; Choi, Jeong June

doi:10.1016/j.jphs.2019.03.009

Cited by 16 publications

(10 citation statements)

References 21 publications

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“…Moreover, BV presents other biological activities such as anti-microbial [15], anti-apoptotic or anti-secretory activity [19]. One of the most attractive compounds in BV is the aforementioned melittin, which will be described later, and that exhibits anticancer effects [61] and enhances muscle regeneration [62]. Studies of the therapeutic effects of BV in diseases like rheumatoid arthritis (RA), Parkinson's disease (PD), multiple sclerosis (MS) and liver fibrosis (LF) have already been performed [3] and reflect a hopeful future for their treatments.…”

Section: Biological Activities Of Bee Venommentioning

confidence: 99%

Bee Venom: An Updating Review of Its Bioactive Molecules and Its Health Applications

2020

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Bee venom (BV) is usually associated with pain since, when humans are stung by bees, local inflammation and even an allergic reaction can be produced. BV has been traditionally used in ancient medicine and in acupuncture. It consists of a mixture of substances, principally of proteins and peptides, including enzymes as well as other types of molecules in a very low concentration. Melittin and phospholipase A2 (PLA2) are the most abundant and studied compounds of BV. Literature of the main biological activities exerted by BV shows that most studies focuses on the comprehension and test of anti-inflammatory effects and its mechanisms of action. Other properties such as antioxidant, antimicrobial, neuroprotective or antitumor effects have also been assessed, both in vitro and in vivo. Moreover, human trials are necessary to confirm those clinical applications. However, notwithstanding the therapeutic potential of BV, there are certain problems regarding its safety and the possible appearance of adverse effects. On this perspective, new approaches have been developed to avoid these complications. This manuscript is aimed at reviewing the actual knowledge on BV components and its associated biological activities as well as the latest advances on this subject.

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Section: Biological Activities Of Bee Venommentioning

confidence: 99%

Bee Venom: An Updating Review of Its Bioactive Molecules and Its Health Applications

2020

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“…Mito-DAMPs may be capable of initiating this pathway as stimulation of mouse splenocytes with cytochrome C in vitro activated NFκB/TNF/IL-6 and MCP-1 (4). In regard to cytokine therapeutics following skeletal muscle stressors, administration of melittin, and a component of bee venom, following muscle injury attenuates MCP-1 plasma levels as well as TNF-a and IL-6 levels at the foci of damage associated with concurrent upregulation of myofibril regeneration biomarkers and improved morphological recovery (40).…”

Section: Il-6 Signaling In Skeletal Musclementioning

confidence: 99%

Mitochondria-cytokine crosstalk following skeletal muscle injury and disuse: a mini-review

Qualls

Southern

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2021

American Journal of Physiology-Cell Physiology

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Skeletal muscle mitochondria are highly adaptable, highly dynamic organelles that maintain the functional integrity of the muscle fiber by providing ATP for contraction and cellular homeostasis (e.g., Na+/K+ ATPase). Emerging as early modulators of inflammation, mitochondria sense and respond to cellular stress. Mitochondria communicate with the environment, in part, by release of physical signals called mitochondrial-derived damage-associated molecular patterns (mito-DAMPs) and deviation from routine function (e.g. reduced ATP production, Ca2+ overload). When skeletal muscle is compromised, mitochondria contribute to an acute inflammatory response necessary for myofibril regeneration; however, exhaustive signaling associated with altered or reduced mitochondrial function can be detrimental to muscle outcomes. Here we describe changes in mitochondrial content, structure, and function following skeletal muscle injury and disuse and highlight the influence of mitochondrial-cytokine crosstalk on muscle regeneration and recovery. While the appropriate therapeutic modulation following muscle stressors remains unknown, retrospective gene expression analysis reveal interleukin-6 (IL-6), interleukin-1b (IL-1b), chemokine C-X-C motif ligand 1 (CXCL1), and monocyte chemoattractant protein 1 (MCP-1) are significantly upregulated following three unique muscle injuries. These cytokines modulate mitochondrial function and execute bona fide pleiotropic roles that can aid functional recovery of muscle; however, when aberrant, chronically disrupt healing partly by exacerbating mitochondrial dysfunction. Multidisciplinary efforts to delineate the opposing regulatory roles of inflammatory cytokines in the muscle-mitochondrial environment are required to modulate regenerative behavior following skeletal muscle injury or disuse. Future therapeutic directions to consider include quenching or limited release of mito-DAMPs and cytokines present in cytosol or circulation.

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“…HBV is under investigation for different diseases as a fundamental or adjuvant treatment, such as Parkinson’s disease, amyotrophic lateral sclerosis, multiple sclerosis, hepatic fibrosis, atherosclerosis, acne vulgaris, pain, lupus nephritis, periodontal diseases, and several cancers. HBV acts on many inflammatory pathways that can affect the body’s immune system and enhance the differentiation of human regulatory T cells, which play an essential role in controlling SARS-CoV infections [ 2 , 12 , 13 , 14 , 15 , 16 , 17 , 18 ]. The presence of high levels of melittin also potentiates the use of HBV as a fungicide, with a broad inhibition spectrum of the fungal genera, including Aspergillus, Botrytis, Candida, Colletotrichum, Fusarium, Malassezia, Neurospora, Penicillium, Saccharomyces, Trichoderma, Trichophyton, and Trichosporonains [ 19 ].…”

Section: Introductionmentioning

confidence: 99%

Chemical, Cytotoxic, and Anti-Inflammatory Assessment of Honey Bee Venom from Apis mellifera intermissa

et al. 2021

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The venom from Apis mellifera intermissa, the main honey bee prevailing in Morocco, has been scarcely studied, despite its known potential for pharmacological applications. In the present work, we investigated the composition, the anti-inflammatory activity, and the venom’s cytotoxic properties from fifteen honey bee venom (HBV) samples collected in three regions: northeast, central, and southern Morocco. The chemical assessment of honey bee venom was performed using LC-DAD/ESI/MSn, NIR spectroscopy and AAS spectroscopy. The antiproliferative effect was evaluated using human tumor cell lines, including breast adenocarcinoma, non-small cell lung carcinoma, cervical carcinoma, hepatocellular carcinoma, and malignant melanoma. Likewise, we assessed the anti-inflammatory activity using the murine macrophage cell line. The study provides information on the honey bee venom subspecies’ main components, such as melittin, apamin, and phospholipase A2, with compositional variation depending on the region of collection. Contents of toxic elements such as cadmium, chromium, and plumb were detected at a concentration below 5 ppm, which can be regarded as safe for pharmaceutical use. The data presented contribute to the first study in HBV from Apis mellifera intermissa and highlight the remarkable antiproliferative and anti-inflammatory effects of HBV, suggesting it to be a candidate natural medicine to explore.

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Melittin – A bee venom component – Enhances muscle regeneration factors expression in a mouse model of skeletal muscle contusion

Cited by 16 publications

References 21 publications

Bee Venom: An Updating Review of Its Bioactive Molecules and Its Health Applications

Bee Venom: An Updating Review of Its Bioactive Molecules and Its Health Applications

Mitochondria-cytokine crosstalk following skeletal muscle injury and disuse: a mini-review

Chemical, Cytotoxic, and Anti-Inflammatory Assessment of Honey Bee Venom from Apis mellifera intermissa

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