2015
DOI: 10.1007/s12562-015-0912-5
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Melon aroma-producing yeast isolated from coastal marine sediment in Maizuru Bay, Japan

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Cited by 5 publications
(2 citation statements)
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“…Strain DNA extraction was conducted as described by Doyle [21]. The 5.8S rDNA amplification was performed using Internal Transcribed Spacer (ITS), ITS1 () and ITS4 () [22]. PCR was then performed using Phusion high-fidelity PCR master mix (New England BioLabs, Inc., Massachusetts, USA).…”
Section: Methodsmentioning
confidence: 99%
“…Strain DNA extraction was conducted as described by Doyle [21]. The 5.8S rDNA amplification was performed using Internal Transcribed Spacer (ITS), ITS1 () and ITS4 () [22]. PCR was then performed using Phusion high-fidelity PCR master mix (New England BioLabs, Inc., Massachusetts, USA).…”
Section: Methodsmentioning
confidence: 99%
“…Jones [7] stated that studies on marine fungi should be mainly concentrated on 'unidentified species on a range of substrates, marine-derived fungi isolated from soils, sand, and water, plankton, deep-sea sediments, endo-biota of marine algae, uncultured fungi, and cryptic species'. Over the last two decades, several fungal culturebased studies have been conducted to reveal novel fungal taxa in marine environments and on other organisms, including mangrove substrates isolated from seaweed, algae, sponges, and the decaying wood of maritime salt marsh plants [41][42][43][44][45][46][47][48][49][50]. Culture-independent methodologies (CIM), which are mostly based on genome analysis, have been the mainstay of ecological research since 2005.…”
Section: Introductionmentioning
confidence: 99%