Membrane- and cell wall-associated heat shock proteins in two genotypes of barley seedlings

Leone, Antonella; Piro, Gabriella; Leucci, Maria Rosaria; Dalessandro, Giuseppe; Zacheo, G.

doi:10.1080/11263500012331358434

Cited by 13 publications

(12 citation statements)

References 38 publications

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“…HSPs are associated with protein folding, protein translocation across membranes, assembly of oligomeric proteins, modulation of receptor activities, mRNA protection, prevention of enzyme denaturation and their stress-induced aggregation, and with post-stress ubiquitin and chaperonin-aided repair. Based on these functions, HSPs have been termed ''molecular chaperones'' (Georgopoulos and Welch, 1993;Leone et al, 2000). Stem 28 kDa glycoprotein is also known as vegetative storage protein A.…”

Section: Functional Distribution Of Identified Proteinsmentioning

confidence: 99%

Separation and identification of soybean leaf proteins by two-dimensional gel electrophoresis and mass spectrometry

Garrett

Sullivan

et al. 2006

Phytochemistry

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Savithiry, "Separation and identification of soybean leaf proteins by two-dimensional gel electrophoresis and mass spectrometry" (2006 AbstractTo establish a proteomic reference map for soybean leaves, we separated and identified leaf proteins using two-dimensional polyacrylamide gel electrophoresis (2D-PAGE) and mass spectrometry (MS). Tryptic digests of 260 spots were subjected to peptide mass fingerprinting (PMF) by matrix-assisted laser desorption/ionization-time of flight (MALDI-TOF) MS. Fifty-three of these protein spots were identified by searching NCBInr and SwissProt databases using the Mascot search engine. Sixty-seven spots that were not identified by MALDI-TOF-MS analysis were analyzed with liquid chromatography tandem mass spectrometry (LC-MS/MS), and 66 of these spots were identified by searching against the NCBInr, SwissProt and expressed sequence tag (EST) databases. We have identified a total of 71 unique proteins. The majority of the identified leaf proteins are involved in energy metabolism. The results indicate that 2D-PAGE, combined with MALDI-TOF-MS and LC-MS/MS, is a sensitive and powerful technique for separation and identification of soybean leaf proteins. A summary of the identified proteins and their putative functions is discussed.

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Section: Functional Distribution Of Identified Proteinsmentioning

confidence: 99%

Separation and identification of soybean leaf proteins by two-dimensional gel electrophoresis and mass spectrometry

Garrett

Sullivan

et al. 2006

Phytochemistry

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“…Phosphoglycerate kinase (PGK) catalyzes the reversible phosphorylation of 3-phosphoglycerate to 1,3-diphosphoglycerate and functions in the photosynthetic carbon metabolism as well as in glycolysis and gluconeogenesis (McMorrow and Bradbeer 1990;Kopke-Secundo et al 1990). Other identified proteins such as HSPs are believed to be associated with protein translation, folding, protein translocation, assembly of oligomeric proteins, modulation of receptor activities, mRNA protection, prevention of enzyme denaturation and stress-induced aggregation (Leone et al 2000). Additional identified protein spots associated with redox maintenance include monodehydroascorbate reductase, anionic peroxidise and 2-cys peroxiredoxin-like protein.…”

Section: Discussionmentioning

confidence: 98%

Proteomic approach to study leaf proteins in a fast-growing tree species, Gmelina arborea Linn. Roxb

Rasineni

Chinnaboina

Reddy

2009

Trees

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Foliar proteome studies have become highly significant for a comprehensive understanding of complex processes associated with plant growth and development.In the present study, we present a proteomic approach to analyze leaf proteins in an important timber-yielding and fast-growing forest tree species, Gmelina arborea Linn. Roxb. (Verbanaceae). Foliar protein analysis involved protein extraction, two-dimensional electrophoresis (2-DE) and matrix-assisted laser desorption/ionization time of flight (MALDI-TOF-TOF). From the 2-DE protein profile of Gmelina leaves, we identified and isolated 150 well-separated protein spots; among these, 64 protein spots were identified by mass spectrometric (MS/MS) analysis. These proteins were classified according to their involvement in basic biological functions, such as photosynthesis, amino acid metabolism, cytoskeleton, cell wall metabolism, stress-related proteins, redox maintenance, electron transport chain, phytohormone metabolism and protein translation and folding. Analytical variance was determined for the protein spots of samples from different plants. The present study is believed to provide a foundation for the use of leaf proteomics in addressing fundamental physiological and biochemical processes associated with growth and productivity of tree species such as Gmelina arborea.

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“…Immunity and defense proteins are stress-related and are important in cell activities, including stabilizing protein folding in response to stimuli, preventing denaturation or refolding of denatured proteins, and regulating many signal transduction pathways (Georgopoulos and Welch, 1993;Leone et al, 2000;Muccilli et al, 2009).…”

Section: Discussionmentioning

confidence: 99%

Separation and identification of Musa acuminate Colla (banana) leaf proteins by two-dimensional gel electrophoresis and mass spectrometry

Lu¹,

Qi²,

Zhang³

et al. 2013

Genet. Mol. Res.

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ABSTRACT. To establish a proteomic reference map of Musa acuminate Colla (banana) leaf, we separated and identified leaf proteins using two-dimensional polyacrylamide gel electrophoresis (2D-PAGE) and mass spectrometry (MS). Tryptic digests of 44 spots were subjected to peptide mass fingerprinting (PMF) by matrixassisted laser desorption/ionization time-of-flight (MALDI-TOF) MS. Three spots that were not identified by MALDI-TOF MS analysis were identified by searching against the NCBInr, SwissProt, and expressed sequence tag (EST) databases. We identified 41 unique proteins. The majority of the identified leaf proteins were found to be involved in energy metabolism. The results indicate that 2D-PAGE is a sensitive and powerful technique for the separation and identification of Musa leaf proteins. A summary of the identified proteins and their putative functions is discussed.

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Membrane- and cell wall-associated heat shock proteins in two genotypes of barley seedlings

Cited by 13 publications

References 38 publications

Separation and identification of soybean leaf proteins by two-dimensional gel electrophoresis and mass spectrometry

Separation and identification of soybean leaf proteins by two-dimensional gel electrophoresis and mass spectrometry

Proteomic approach to study leaf proteins in a fast-growing tree species, Gmelina arborea Linn. Roxb

Separation and identification of Musa acuminate Colla (banana) leaf proteins by two-dimensional gel electrophoresis and mass spectrometry

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