Membrane chromatographic test system for the determination of bisphenol A in drinking water based on the use of an aptamer

Abstract

A membrane test system has been developed for the rapid determination of bisphenol A in drinking water, utilizing a conjugate of gold nanoparticles with an aptamer that specifically binds the target analyte, and a conjugate of mercaptosuccinic acid with a carrier protein impregnated in the test zone of the strip. The working principle of the test system is based on the binding of free gold nanoparticles in the test zone, which are formed as a result of the competitive interaction of the aptamer with bisphenol A and its release from the surface of the gold nanoparticles. Conjugates of gold nanoparticles with aptamers of different compositions were obtained and tested. Optimal conditions were selected to achieve a low detection limit for bisphenol A. The developed test system allows for the detection of bisphenol A within 15 minutes with a detection limit of 13.5 ng/mL. The suitability of the test system was confirmed by testing drinking water; the detection rate of bisphenol A ranged from 88.2 to 101.3%.