Membrane-Coating Granules

Hayward, A.F.

doi:10.1016/s0074-7696(08)61661-7

Cited by 72 publications

(22 citation statements)

References 122 publications

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“…9a-c). They resemble the type of MCG as described for the oral mucosa [Squier, 1977;Hayward, 1979;Squier and Hall, 1984], for the human cervix [Grubb et al, 1968], the nonhuman primate vagina [King, 1983[King, , 1985a and the shrew vagina [Kress and Milian, 1987]. King [1985a] and Grubb et al [1968] describe the presence of both types of MCG in some cervix and vagina cells.…”

Section: Discussionmentioning

confidence: 67%

Changes in the Epithelium of the Vaginal Complex during the Estrous Cycle of the Marsupial <i>Monodelphis domestica</i>

Regli

Kress²

2002

Cells Tissues Organs

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The vaginal complex of marsupials differs from that of eutherians. Cervices open separately in a sinus vaginalis or cul-de-sac. Two lateral vaginae adjoin the sinus vaginalis and fuse at the level of the urethra opening and form the sinus urogenitalis. During the estrous cycle the vaginal epithelium undergoes a number of specified morphological changes. This paper is the first to describe these changes on an ultrastructural level in a marsupial. Investigations in Monodelphis vagina reveal that a cyclic switch exists between a keratinized and a stratified nonkeratinized epithelium. Keratinization starts during proestrus and reaches its maximum during estrus. In the postestrus, desquamation of the stratum corneum takes place, mostly in two steps. In metestrus one to two additional layers of the now nonkeratinized surface cells are shed into the vaginal lumen. Typical cell structures, such as keratin filaments, keratohyalin and membrane-coating granules, are involved in the keratinization process. Keratohyalin is found in the cytoplasm as well as in the nucleus of stratum granulosum cells, a phenomenon which is known from other parakeratinized epithelia of rapid turnover. Membrane-coating granules, responsible for the permeability barrier between the epithelial cells, are of the nonlamellated type in the nonkeratinized epithelium and produce an amorphous material in the intercellular spaces after extrusion. At periods, however, when the epithelium is keratinized, membrane-coating granules are of the lamellated type and form a lamellated barrier structure after extrusion in the intercellular space. The loss of the protective keratinized layers asks for an additional defense mechanism for the epithelium. The migration of leukocytes through the epithelium predominantly during post- and metestrus and their presence in the vaginal lumen may play a protective role together with the bacterial content.

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Section: Discussionmentioning

confidence: 67%

Changes in the Epithelium of the Vaginal Complex during the Estrous Cycle of the Marsupial <i>Monodelphis domestica</i>

Regli

Kress²

2002

Cells Tissues Organs

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“…The observations that LBs contain lysosomal enzymes like cathepsin D (3,4) and vacuolar H ϩ -ATPase and have acidic contents (5) have led them to be considered as lysosomerelated organelles. They are produced in the keratinocytes of the upper stratum spinosum and are more abundant in the cytoplasm of the granular keratinocytes (6,7). They secrete their contents, lipids (glucosylceramides, sphingomyelin, phospholipids, and cholesterol) and lipid-processing enzymes (e.g.…”

mentioning

confidence: 99%

Lamellar Bodies of Human Epidermis

Raymond

Peredo

Stella

et al. 2008

Molecular & Cellular Proteomics

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“…Lamellar granules (LG), also known as keratinosomes, lamellar bodies, membrane-coating granules, and Odland bodies, are specialized secretory granules of the keratinizing stratified squamous epithelia and are thought to be essential in barrier formation and desquamation (Hayward, 1979;Odland and Holbrook, 1981). LG components include glucosylceramides (GlcCer) and other lipids (Madison et al, 1998), various hydrolytic enzymes, such as proteases, acid phosphatases, glucosidases, and lipases (Wolff and Schreiner, 1970;Hayward, 1979;Traczyk, 1983, 1985;Grayson et al, 1985;Madison et al, 1998), and other proteins including corneodesmosin (Cdsn) (Serre et al, 1991).…”

mentioning

confidence: 99%

Epidermal Lamellar Granules Transport Different Cargoes as Distinct Aggregates

Ishida‐Yamamoto

Simon

Kishibe

et al. 2004

Journal of Investigative Dermatology

138

113

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Lamellar granules (LG) of the epidermis appear as discrete round or oblong shaped granules in classical transmission electron micrographs, but a recent cryo-transmission electron microscopy study has claimed that LG are in fact branched tubular structures. LG contain various cargoes including lipids, hydrolytic enzymes, and several other proteins. It is not known whether there are any differences in the timing of expression among them and whether they are sorted into the granules individually or collectively. In order to address these questions, we studied the expression of glucosylceramides (GlcCer), cathepsin D (CatD), corneodesmosin (Cdsn), kallikrein (KLK)7, and KLK8 in normal human epidermis using confocal laser scanning microscopy and immunoelectron microscopy. The results were consistent with the model that LG are parts of a branched tubular structure. In this structure, all the components were shown to be distributed as separate aggregates. In the trans-Golgi network (TGN), bulbous protrusions containing GlcCer, Cdsn, KLK7 and KLK8, and small CatD-positive vesicles were observed. The molecules were shown to be delivered to the apical region of granular keratinocytes. This study provides strong evidence for the sequential synthesis and independent trafficking of various LG cargoes, including for the first time CatD and KLK8, from TGN.

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Membrane-Coating Granules

Cited by 72 publications

References 122 publications

Changes in the Epithelium of the Vaginal Complex during the Estrous Cycle of the Marsupial <i>Monodelphis domestica</i>

Changes in the Epithelium of the Vaginal Complex during the Estrous Cycle of the Marsupial <i>Monodelphis domestica</i>

Lamellar Bodies of Human Epidermis

Epidermal Lamellar Granules Transport Different Cargoes as Distinct Aggregates

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