Membrane-Cytoskeleton Dynamics in the Course of Mast Cell Activation

Abstract

Aggregation of the high-affinity IgE receptor (FcεRI) on the plasma membrane of mast cells and basophils initiates signaling events leading to a rapid release of preformed inflammatory mediators from secretory granules, and overall changes in cell morphology. Mast cell activation also causes reorganization of cytoskeletal components associated with membrane ruffling, spreading, and migration. Here we describe methods used for visualization of mast cell cytoskeleton, focusing on its two major components, microfilaments and microtubules, and their changes after cell triggering.

Kinesin-1 controls mast cell degranulation and anaphylaxis through PI3K-dependent recruitment to the granular Slp3/Rab27b complex

“…Degranulation is accompanied by the extensive reorganization of the cytoskeleton associated with membrane ruffling and spreading (Dráber and Dráber, 2015). The degranulation process also involves the anterograde movement of SGs toward Cross-linking of mast cell (MC) IgE receptors (FcεRI) triggers degranulation of secretory granules (SGs) and the release of many allergic and inflammatory mediators.…”

Long-Tailed Unconventional Class I Myosins in Health and Disease

“…These data point to a model where MYO1F could serve as a link between the actin cytoskeleton and the localization and function of integrin in the cell membrane after the activation of KIT receptors (Figure 2). We further hypothesize that MYO1F could modulate integrin by regulating the cortical actin mesh, on the basis of evidence that the cortical actin ring is necessary for mast cell migration [48]. Myo1E has not been reported in mast cells to date.…”