Membrane localization of a rice calcium-dependent protein kinase (CDPK) is mediated by myristoylation and palmitoylation

Martín, Mariana; Busconi, Liliana

doi:10.1046/j.1365-313x.2000.00889.x

Cited by 176 publications

(114 citation statements)

References 31 publications

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“…1 with underlines. The N-terminal putative myristoylation site (MGxxxSxx, PlantsP database) is conserved within plant CPKs superfamily [7][8][9][10], which may be required for the subcellular localization of NtCPK5.…”

Section: Molecular Cloning Of Ntcpk5mentioning

confidence: 99%

“…A putative myristoylation site (MGxxxSxx, PlantsP database) at the beginning of the NCPK5 coding region, which is also present in other plant CPKs [7][8][9][10], was presumed involving in the subcellular localization of NtCPK5.…”

Section: Molecular Cloning Of Ntcpk5mentioning

confidence: 99%

“…The N-terminal variable region has been shown essential for the subcellular localization of many CPKs [7][8][9][10]. (Fig.…”

Section: Subcellular Localization Of Ntcpk5mentioning

confidence: 99%

“…Except of the three highly conserved domains, CPKs also have an N-terminal variable region, which often contains N-terminal acylation (including myristoylation and palmitoylation) sites required for the subcellular localization of certain CPKs [7][8][9][10]. Myristoylation often occurs at Gly residue at position 2 and may or may not sufficient for membrane anchoring.…”

Section: Introductionmentioning

confidence: 99%

“…Myristoylation often occurs at Gly residue at position 2 and may or may not sufficient for membrane anchoring. Palmitoylation occurred at Cys residue at position 4 as a supplement can promote and stabilize the targeting of CPKs to plasma membrane [7][8][9]. In Arabidopsis, there are 34 CPK isoforms, among them, only five AtCPKs lack both Gly2 and Cys4.…”

Section: Introductionmentioning

confidence: 99%

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Cellular localization and biochemical characterization of a novel calcium-dependent protein kinase from tobacco

Wang

Zhang

et al. 2005

Cell Res

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By screening tobacco cDNA library with MCK1 as a probe, we isolated a cDNA clone NtCPK5 (accession number AY971376), which encodes a typical calcium-dependent protein kinase. Sequence analyses indicated that NtCPK5 is related to both CPKs and CRKs superfamilies and has all of the three conserved domains of CPKs. The biochemical activity of NtCPK5 was calcium-dependent. NtCPK5 had V max and K m of 526 nmol/min/mg and 210 µg/ml respectively with calf thymus histone (fraction III, abbreviated to histone IIIs) as substrate. For substrate syntide-2, NtCPK5 showed a higher V max of 2008 nmol/min/mg and a lower K m of 30 µM. The K 0.5 of calcium activation was 0.04 µM or 0.06 µM for histone IIIs or syntide-2 respectively. The putative myristoylation and palmitoylation consensus sequence of NtCPK5 suggests that it could be a membrane-anchoring protein. Indeed, our transient expression experiments with wild type and mutant forms of NtCPK5/GFP fusion proteins showed that NtCPK5 was localized to the plasma membrane of onion epidermal cells and that the localization required the N-terminal acylation sites of NtCPK5/GFP. Taking together, our data have demonstrated the biochemical characteristics of a novel protein NtCPK5 and its subcellular localization as a membrane-anchoring protein.

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Section: Molecular Cloning Of Ntcpk5mentioning

confidence: 99%

Section: Molecular Cloning Of Ntcpk5mentioning

confidence: 99%

“…The N-terminal variable region has been shown essential for the subcellular localization of many CPKs [7][8][9][10]. (Fig.…”

Section: Subcellular Localization Of Ntcpk5mentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Cellular localization and biochemical characterization of a novel calcium-dependent protein kinase from tobacco

Wang

Zhang

et al. 2005

Cell Res

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Molecular Bases of Plant Adaptation to Abiotic Stress and Approaches to Enhance Tolerance to Hostile Environments

Coraggio

Tuberosa

2004

Handbook of Plant Biotechnology

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Abiotic stress has become the greatest threat to global food production and security, particularly in developing countries. Progress in increasing yield and its stability under adverse conditions through a direct selection has been hampered by the low heritability of yield, especially under drought. A better knowledge of the molecular and genetic basis of the mechanisms promoting tolerance to abiotic stress will enhance our capacity to more appropriately manipulate the genes limiting crop yield under hostile environments. This review describes and critically analyses the major advances in our understanding of the complex mechanisms underlying the adaptive response of the plant to abiotic stress, starting with the perception of the stress at the cellular level, its transduction through a cascade of signalling molecules and ending with the induction of genes whose products mitigate the harmful effects of the stress. Molecular markers and the ‘omics’ platforms (e.g., transcriptomics, proteomics, metabolomics etc.) offer unprecedented opportunities to identify and clone the genes governing plant adaptation to environmental stress and limiting yield under such conditions. The dissection of the genetic basis of quantitative traits into their single components, the so‐called QTLs (quantitative trait loci), allows us to deploy marker‐assisted selection (MAS) for enhancing crop performance under adverse conditions. Additional opportunities are also offered by genetic engineering, particularly through the expression of transcription factors regulating a suite of genes whose product is crucial to mitigate the effects of stress. Although the applicative results so far achieved with non‐conventional approaches have been limited, their integration with conventional breeding methodologies and other interdisciplinary approaches will greatly enhance our capacity to more effectively tailor crop genome to withstand harsh environmental conditions and to improve their sustainability under marginal conditions.

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Paradigms and Networks for Intracellular Calcium Signaling in Plant Cells

Luan

2018

Annual Plant Reviews Online

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Calcium (Ca2+) is a ubiquitous second messenger in all eukaryotes. A challenging question is how the cation serves as a messenger for numerous signals and confers specificity to cellular responses. Recent studies have established a concept called “calcium signature” that specifies calcium changes triggered by each signal. Along with this concept comes the “decoding” of calcium signatures—how cells recognize and translate the signatures (codes) into the correct cellular responses. The initial step in the decoding process involves sensor proteins that bind calcium and change their conformation, leading to activation or inactivation of the downstream target molecules thereby regulating the specific biochemical and physiological processes. In this chapter, studies on the major groups of calcium sensors including calcium‐dependent protein kinases (CDPKs), calmodulin (CaM), and calcineurin B‐like proteins (CBLs) will be summarized and general paradigms and networks of calcium signaling processes will be discussed. I will particularly focus on the more recent studies that have advanced our understanding on calcium signal transduction in plants.

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Membrane localization of a rice calcium-dependent protein kinase (CDPK) is mediated by myristoylation and palmitoylation

Cited by 176 publications

References 31 publications

Cellular localization and biochemical characterization of a novel calcium-dependent protein kinase from tobacco

Cellular localization and biochemical characterization of a novel calcium-dependent protein kinase from tobacco

Molecular Bases of Plant Adaptation to Abiotic Stress and Approaches to Enhance Tolerance to Hostile Environments

Paradigms and Networks for Intracellular Calcium Signaling in Plant Cells

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