Membrane mixing and dynamics in hybrid POPC/poly(1,2-butadiene-<i>block</i>-ethylene oxide) (PBd-<i>b</i>-PEO) lipid/block co-polymer giant vesicles

Seneviratne, Rashmi; Catania, Rosa; Rappolt, Michael; Jeuken, Lars J. C.; Beales, Paul A.

doi:10.1039/d1sm01591e

Cited by 17 publications

(30 citation statements)

References 30 publications

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“…We have previously observed that hybrid giant unilamellar vesicles (GUVs) of PBd 22 - b -PEO 14 and 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine (POPC) are well-mixed and homogeneous with a similar molecular ordering and packing, but lower fluidity, than POPC lipid bilayers . Previous works have also shown that the area stretching moduli ( K a ) of polymersomes made of PBd- b -PEO polymers (90–130 mN/m , ) are much lower than the typical K a for phosphocholine liposomes (200–260 mN/m). , For HVs composed of PBd 22 - b -PEO 14 and 1,2-Dioleoyl-sn-glycero-3-phosphocholine (DOPC), or PBd 46 - b -PEO 30 mixed with POPC, the area stretching modulus lies intermediate between that of pure polymer and pure lipid vesicles. , While comparable data are not available for mixtures of E. coli polar lipid extract and PBd 22 - b -PEO 14 , we infer that the block copolymer will impart a similar reduction in the stretching modulus of vesicles in this work.…”

Section: Discussion and Conclusionmentioning

confidence: 99%

Detergent-Free Functionalization of Hybrid Vesicles with Membrane Proteins Using SMALPs

et al. 2022

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Hybrid vesicles (HVs) that consist of mixtures of block copolymers and lipids are robust biomimetics of liposomes, providing a valuable building block in bionanotechnology, catalysis, and synthetic biology. However, functionalization of HVs with membrane proteins remains laborious and expensive, creating a significant current challenge in the field. Here, using a new approach of extraction with styrene-maleic acid (SMA), we show that a membrane protein (cytochrome bo 3 ) directly transfers into HVs with an efficiency of 73.9 ± 13.5% without the requirement of detergent, long incubation times, or mechanical disruption. Direct transfer of membrane proteins using this approach was not possible into liposomes, suggesting that HVs are more amenable than liposomes to membrane protein incorporation from a SMA lipid particle system. Finally, we show that this transfer method is not limited to cytochrome bo 3 and can also be performed with complex membrane protein mixtures.

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Section: Discussion and Conclusionmentioning

confidence: 99%

Detergent-Free Functionalization of Hybrid Vesicles with Membrane Proteins Using SMALPs

et al. 2022

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“…The combined effect of the bending and Gaussian curvature moduli indicates that polymer-rich membranes result in more stable vesicles, as confirmed by the fluorescence leakage experiments under addition of detergent. Therefore, as noted in several publications, 13,[17][18][19]22,24,26 HVs are robust structures that can be used as effective alternatives to pure polymersomes and liposomes. To explore the interaction of HV with membrane proteins, the following section will focus on the dynamics of pure and hybrid vesicles with the WALP 23 peptide.…”

Section: Mechanical Behaviormentioning

confidence: 92%

“…This is an interesting observation given that earlier fluorescence studies have shown that POPC and PBd 22 -b-PEO 14 form well mixed and homogeneous vesicles containing both polymers and lipids in their composition. 26 We previously hypothesized that the co-existence of these two distinct membrane structures in otherwise homogeneously mixed membranes indicates the thin and thick structures have comparable free energies, while intermediate structures are energetically unfavorable. 22 The CG representation in the present study appears to accurately model the two distinct populations of HVs.…”

Section: Structural Propertiesmentioning

confidence: 99%

“…The individual diffusivity of POPC is higher than that of PBd-b-PEO, which is expected due to the reduced free volume required for lipids to hop laterally between sites in the membrane matrix. 26 Moreover, higher temperatures lead to more fluid membranes as a result of decreased viscosity and increased molecular mobility. 37 The simulated values are in good agreement with the diffusivity range measured experimentally for pure POPC membranes at room temperature using nuclear magnetic resonance (3.2 × 10 −8 -1.9 × 10 −7 cm 2 s −1 ).…”

Section: Structural Propertiesmentioning

confidence: 99%

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Unraveling the Phase Behavior, Mechanical Stability, and Protein Reconstitution Properties of Polymer-Lipid Hybrid Vesicles

Müller

Beales

Muniz

et al. 2023

Preprint

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Hybrid vesicles consisting of natural phospholipids and synthetic amphiphilic copoly- mers have shown remarkable material properties and potential for biotechnology, com- bining the robustness of polymers with the biocompatibility of phospholipid mem- branes. To predict and optimize the mixing behavior of lipids and copolymers, as well as understand the interaction between the hybrid membrane and macromolecules like membrane proteins, a comprehensive understanding at the molecular level is essen- tial. This can be achieved by a combination of molecular dynamics simulations and experiments. Here, simulations of POPC and PBd22-b-PEO14 hybrid membranes are shown, uncovering different copolymer configurations depending on the polymer-to- lipid ratio. High polymer concentrations created thicker membranes with an extended polymer conformation, while high lipid content led to the collapse of the polymer chain. High concentration of polymer further correlated with a decreased area com- pression modulus and altered lateral pressure profiles, hypothesized to result in the experimentally-observed improvement in membrane protein reconstitution and resis- tance towards destabilization by detergents. Finally, simulations of a WALP peptide embedded in the bilayer showed that only membranes with up to 50% polymer con- tent favored a transmembrane configuration. These simulations correlate with previous and new experimental results and provide a deeper understanding of the properties of lipid-copolymer hybrid membranes.

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“…When mixing the noncrystalline polymer PBd- b -PEO with the phospholipid POPC, no evidence for the formation of lipid-rich or polymer-rich domains was found. Membrane fluidity was shown to decrease with increasing polymer fraction by a factor of five to seven, depending on the polymer length [ 66 ]. While polymeric membranes are promising candidates for the creation of artificial cell membranes, using hybrid lipid–polymer cell membrane models combines the chemical versatility and robustness of polymers with the biocompatibility of lipids and allows for the formation of membrane domains for spatial organization.…”

Section: Applications Of Compartments In the Biomedical Fieldmentioning

confidence: 99%

Current Perspectives on Synthetic Compartments for Biomedical Applications

Heuberger

Korpidou

Eggenberger

et al. 2022

IJMS

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Nano- and micrometer-sized compartments composed of synthetic polymers are designed to mimic spatial and temporal divisions found in nature. Self-assembly of polymers into compartments such as polymersomes, giant unilamellar vesicles (GUVs), layer-by-layer (LbL) capsules, capsosomes, or polyion complex vesicles (PICsomes) allows for the separation of defined environments from the exterior. These compartments can be further engineered through the incorporation of (bio)molecules within the lumen or into the membrane, while the membrane can be decorated with functional moieties to produce catalytic compartments with defined structures and functions. Nanometer-sized compartments are used for imaging, theranostic, and therapeutic applications as a more mechanically stable alternative to liposomes, and through the encapsulation of catalytic molecules, i.e., enzymes, catalytic compartments can localize and act in vivo. On the micrometer scale, such biohybrid systems are used to encapsulate model proteins and form multicompartmentalized structures through the combination of multiple compartments, reaching closer to the creation of artificial organelles and cells. Significant progress in therapeutic applications and modeling strategies has been achieved through both the creation of polymers with tailored properties and functionalizations and novel techniques for their assembly.

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Membrane mixing and dynamics in hybrid POPC/poly(1,2-butadiene-block-ethylene oxide) (PBd-b-PEO) lipid/block co-polymer giant vesicles

Abstract: Homogeneous hybrid vesicles where lipid diffusion decreases with increasing polymer content but polymer diffusion is minimally affected by lipid composition.

Cited by 17 publications

References 30 publications

Detergent-Free Functionalization of Hybrid Vesicles with Membrane Proteins Using SMALPs

Detergent-Free Functionalization of Hybrid Vesicles with Membrane Proteins Using SMALPs

Unraveling the Phase Behavior, Mechanical Stability, and Protein Reconstitution Properties of Polymer-Lipid Hybrid Vesicles

Current Perspectives on Synthetic Compartments for Biomedical Applications

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