Membrane open-system cell for oxygen consumption measurement

Титовец, Э. П.

doi:10.1016/0003-2697(87)90548-3

Cited by 6 publications

(4 citation statements)

References 5 publications

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“…To determine the appropriate anticancer therapeutic in the case of hypoxic tumor environments, probes capable of reporting on the oxygen status within that tissue are essential. Several methods to detect hypoxia have been developed, such as blood flow velocity monitoring, [15] Clark electrode, [16] nuclear magnetic resonance (NMR), [17,18] electron paramagnetic resonance (EPR), [19] positron emission tomographic (PET) imaging, [20] and fluorescence imaging. Among them, fluorescence imaging based on specific probes for hypoxia markers is emerging as a powerful method for rapid visualization and real‐time monitoring of tumor hypoxia [2,21–24] …”

Section: Introductionmentioning

confidence: 99%

Small Molecule Fluorescent Probes for Sensing and Bioimaging of Nitroreductase

et al. 2022

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Hypoxia is a condition caused by a decrease in oxygen and is often seen in cases of cardiac ischemia, solid tumors, inflammation, and other diseases. Tumor hypoxia can result in cancer invasion, metastasis, decreased apoptosis, and resistance to various therapies (chemotherapy, immunotherapy, and radiotherapy), ultimately reducing overall survival rates. These effects of hypoxia underline the need to monitor hypoxia‐associated specific markers for more effective therapeutic intervention. Nitroreductase (NTR) is a common biomarker of hypoxia, via its reduction activity with nitroaromatic compounds to corresponding amino derivatives using NADH and NADPH as cofactor. The NTR expression level can be correlated to cellular hypoxia. Therefore, developing a diagnostic tool for measuring NTR activity is of immense importance for improving treatment efficacies. To leverage NTR activity as a proxy of hypoxia, small molecule based fluorescent probes have been developed as quantifiable probes. To date, several NTR‐sensitive fluorescent probes have been reported. This review is focuses on the advances in these fluorescent NTR probes over the last five years.

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Section: Introductionmentioning

confidence: 99%

Small Molecule Fluorescent Probes for Sensing and Bioimaging of Nitroreductase

et al. 2022

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“…3,4 Clark electrodes have been widely used to measure extracellular oxygen level in cell culture media. 1,5 However, when applied in intracellular studies, the electrodes may injure the cell due to the penetration. A Clark microelectrode can only measure the oxygen level in one cell at a time and is not suitable in applications that require fast cell screening.…”

Section: Introductionmentioning

confidence: 99%

Novel fluorescent oxygen indicator for intracellular oxygen measurements

Rosenzweig

Jones

et al. 2002

J. Biomed. Opt.

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Intracellular oxygen concentration is of primary importance in determining numerous physiological and pathological processes in biological systems. In this paper, we describe the application of the oxygen sensing indicator, ruthenium dibipyridine 4-(1"-pyrenyl)-2,2'-bipyridine chloride [Ru(bpy-pyr)(bpy)(2)], for molecular oxygen measurement in J774 murine macrophages. Ru(bpy-pyr)(bpy)(2) exhibits strong visible absorption, efficient fluorescence, long excited state lifetime, large Stokes shift, and high photo- and chemical stability. The fluorescence of Ru(bpy-pyr)(bpy)2 is efficiently quenched by molecular oxygen. It is 13 fold higher in a nitrogenated solution than in an oxygenated one. The dye passively permeates into cells and maintains its oxygen sensitivity for at least 5 h when the cells are stored in a phosphate buffered saline solution at pH 7.4. The oxygen sensitivity, photostability, and chemical stability of the indicator and the effect of hypoxia and hyperoxia on the intracellular oxygen level in single macrophages are discussed.

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“…8,9 Clark electrodes have been widely used to measure extracellular oxygen levels in cell culture media. 6,10 However, when applied to intracellular studies, the electrodes may cause structural damage to the cell membrane due to penetration. The use of oxygen microelectrodes only enables the measurement of molecular oxygen in one cell at a time and is not suitable for applications that require fast cell screening.…”

mentioning

confidence: 99%

“…Despite the significance of intracellular oxygen in various biochemical processes, there are surprisingly few analytical methods to measure its levels. , The role of oxygen in cellular processes is mostly assessed by indirect data derived from the measurement of extracellular oxygen concentration. This approach is ambiguous, as there is a difference between intracellular and extracellular oxygen concentrations. , Clark electrodes have been widely used to measure extracellular oxygen levels in cell culture media. , However, when applied to intracellular studies, the electrodes may cause structural damage to the cell membrane due to penetration. The use of oxygen microelectrodes only enables the measurement of molecular oxygen in one cell at a time and is not suitable for applications that require fast cell screening.…”

mentioning

confidence: 99%

Molecular Oxygen-Sensitive Fluorescent Lipobeads for Intracellular Oxygen Measurements in Murine Macrophages

Rosenzweig

Jones

et al. 2001

Anal. Chem.

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Intracellular oxygen concentration is of primary importance in determining numerous physiological and pathological processes in biological systems. This paper describes the development and application of micrometer-sized oxygen-sensitive fluorescence lipobeads for intracellular measurements of molecular oxygen in J774 murine macrophages. A ruthenium diimine complex [Ru(bpy-pyr)(bpy)2]C12 (bpy = 2,2'-bipyridine, bpy-pyr = 4-(1"-pyrenyl)-2,2'-bipyridine) is used as the oxygen indicator. The indicator exhibits high chemical and photostability and high sensitivity to oxygen. The indicator molecules are immobilized in a phospholipid membrane that coats polystyrene microparticles. The fluorescence of the lipobeads is effectively quenched by molecular oxygen. The fluorescence intensity of the oxygen-sensitive lipobeads is 3 times higher in a nitrogenated solution than in an oxygenated solution. The lipobeads are internalized by murine macrophages through phagocytosis. They maintain their spectral properties for 24 h in living cells when the cells are stored in phosphate-buffered saline at pH 7.4. The photostability, reversibility, and effect of hypoxia, hyperoxia, and oxidative stress on the intracellular level of oxygen in J774 murine macrophages are described.

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Membrane open-system cell for oxygen consumption measurement

Cited by 6 publications

References 5 publications

Small Molecule Fluorescent Probes for Sensing and Bioimaging of Nitroreductase

Small Molecule Fluorescent Probes for Sensing and Bioimaging of Nitroreductase

Novel fluorescent oxygen indicator for intracellular oxygen measurements

Molecular Oxygen-Sensitive Fluorescent Lipobeads for Intracellular Oxygen Measurements in Murine Macrophages

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