Membrane stabilizing activity — a possible mechanism of action for the anti-inflammatory activity of Cedrus deodara wood oil

Shinde, Ujwala A.; Phadke, A.S; Nair, Anupama; Mungantiwar, Ashish; Dikshit, V.J; Saraf, M. N.

doi:10.1016/s0367-326x(99)00030-1

Cited by 412 publications

(350 citation statements)

References 4 publications

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“…L flower (3.97 RE/100g DW) which was higher than those of Lawsonia inermis L. leaves extract (3.62 g RE/100g DW) and Cuminum cyminum seeds extract (0.52 g RE/100g DW). The result reported by (Tan et al, 2013) for Lawsonia inermis leaves (5.55 g GAE/100 g DW), by (Rebey et al, 2012) for Cuminum cyminum seeds (1.45-1.86 g GAE/100 g DW) and by (Bayram et al, 2015) for Rosa damascena flower (4.31 g GAE/100 g DW) are very close to our results. Several studies link the antioxidant activity of medicinal plant to their phenolics content due to their redox properties, which plays an important role in neutralizing and scavenging free radicals, quenching singlet and triplet oxygen, or decomposing peroxides (Ghasemzadeh et al, 2010).…”

Section: Total Phenolic and Flavonoid Contentsupporting

confidence: 80%

“…The membrane-stabilizing effect was established to increase with the increase drug and plant extract concentration. These plant extracts may act through a deformation of cells via their interaction with membrane proteins (Shinde et al, 1999) or other compound in the erythrocyte membranes, which provoke later alteration of the surface charges of the cells (Oyedapo et al, 2004). Knowing that changing in the form and volume of the cell is much related to the intracellular amount of calcium.…”

Section: The Membrane-stabilizing Potentialmentioning

confidence: 99%

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In vitro Antioxidant and anti-inflammatory properties of selected Moroccan medicinal plants

Bouhlali¹,

Sellam²,

Bammou³

et al. 2016

J App Pharm Sci

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The aim of the present study was to examine the antioxidant and anti-inflammatory activities of three Moroccan ethno medicinally important plants. The antioxidant activity was performed using β-Carotene bleaching assay, DPPH, ABTS and Ferric reducing assays. In vitro anti-inflammatory assays was also studied through the evaluation of membrane stabilization potential and inhibition of protein denaturation. Results revealed that Lawsonia inermis L leaves extract had significantly higher phenolics-content (5.23 g GAE/100g DW) and exhibited the highest antioxidant activity based on DPPH (IC50 = 18.26 µg/mL). The highest flavonoids content was found in Rosa damascena. L flower (3.97 RE/100g DW) which shows the highest antioxidant activity based on ABTS (30.39 mmol TE/100g DW), FRAP (38.54 mmol TE/100gDW) and β-Carotene bleaching assay (IC50 = 56.33 µg/mL). Concerning the anti-inflammatory potency, the highest membrane stabilization effect was found in Rosa damascena. L flower (IC50 = 125.02 µg/mL) and the highest anti-denaturation of protein effect was found in Lawsonia inermis L leaves extract (IC50 = 103.21 µg/mL). Consequently, to conclude flavonoids and related phenolics present in studied plant materials may possibly be responsible for the important antiinflammatory and antioxidant activities.

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Section: Total Phenolic and Flavonoid Contentsupporting

confidence: 80%

Section: The Membrane-stabilizing Potentialmentioning

confidence: 99%

In vitro Antioxidant and anti-inflammatory properties of selected Moroccan medicinal plants

Bouhlali¹,

Sellam²,

Bammou³

et al. 2016

J App Pharm Sci

View full text Add to dashboard Cite

show abstract

“…The method of Shinde et al [33] was used with modifications. MFGM and CFGM at various concentrations (25, 50, 75 and 100 µg) in 2.5 ml distilled water was mixed with equal volumes each of sodium phosphate buffer (0.2 M, pH 6.6) and potassium ferricyanide (1% (w/v) in water) in a test tube and incubated for 20 min at 50 0 C. The mixture was cooled using crushed ice and 0.5 ml of trichloroacetic acid (10% (v/v) in water) was added and the total reaction mixture was centrifuged for 10 min, 3000 rpm, room temperature.…”

Section: Reducing Power Assaymentioning

confidence: 99%

Buffalo (Bubalus bubilis) colostrum and milk fat globule membrane fractions are potent antioxidants

2017

J App Biol Biotech

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Colostrum is the first liquid food for the new born baby, secreted after the parturition. After few days of maturation the colostrum turns to milk. The cream or fat fraction of milk consists of fat droplets composed primarily of triacylglycerols that are secreted from the apical surface of the mammary cells surrounded by cellular thin membranes membrane, the fat globule membrane (FGM), formed by proteins which have been suggested to be cholesterolemia-lowering factors, inhibitors of cancer cell growth, vitamin binders, antioxidant, bactericidal, suppressors of multiple sclerosis. FGM was isolated from buffalo colostrum and milk, the fat content was > 2-fold higher in the former than in the latter. The colostrum fat globule membrane (CFGM) fraction also showed significantly higher protein content than milk fat globule membrane (MFGM) extract. Further, the SDS-PAGE separation of the total proteins from the CFGM and MFGM showed differential banding pattern. Antioxidative property of the two FGM extracts was deliberated based on their free radical scavenging ability and reducing power. CFGM was found to exhibit a relatively higher antioxidant and reductive capacity than the MFGM fractions. The results obtained in the present study showcases the superior beneficial health effects of the CFGM over the MFGM.

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“…40 The precise mechanism of this membrane stabilization is yet to be elucidated; it is possible that the T. ochracea extracts produced this effect surface area/volume ratio of the cells, which could be brought about by an expansion of membrane or the shrinkage of cells and an interaction with membrane proteins. 29 Proteinases have been implicated in arthritic reactions. Neutrophils are known to be a source of proteinase which carries in their lysosomal granules many serine proteinases.…”

Section: Anti-inflammatory Assaymentioning

confidence: 99%

“…29 Test samples were dissolved in 0.25 ml of 2 M borate buffer pH 9.0 and added 0.25 ml of lipoxidase enzyme solution (20,000 U/ ml) and incubated for 5 min at 25 0 C. After which, 1.0 ml of lenoleic acid solution (0.6 mM) was added, mixed well and absorbance was measured at 234 nm. Indomethcin was used as reference standard.…”

Section: Anti-lipoxygenase Activitymentioning

confidence: 99%

Phytochemical analysis and in vitro antioxidant, antimicrobial, anti-inflammatory and cytotoxicity activities of wood rotting fungi, Trametes ochracea

Govindappa¹,

Roshan²,

Nithi³

et al. 2015

Phcog J

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Objective: The present research was aimed to identify the metabolites in the methaolic and hexane extract of Trametes ochracea and evaluated these extracts to know their in vitro biological activities. Materials and methods: Two solvent extracts of T. ochracea was subjected to phytochemical analysis. The two solvent extracts were subjected to in vitro biological activity viz., antioxidant, anti-inflammatory and cytoxicity assays. Results: The methanol extract yielded important phytochemicals viz., saponins, flavonoids, alkaloids, steroids, phenols and tannins compared to hexane. The methanolic extract has shown strong antioxidant activity in all tested methods. The methanol extract was effectively inhibited the heat induced hemolysis, antilipoxygenase activity and also stabilized the membrane, avoided the membrane denaturation, proteinase and xanthine oxidase inhibition.The onion root meristametic cells were inhibited due to toxicity of methanol extract by possessing various cellular abnormalities in various stages of actively growing cells. The yeast cells were dead due to toxicity of methanol extract by possessing cell necrosis and also fragmented the cell DNA. Conclusion: The obtained results clearly indicates that Trametes ochracea methanol extract is having potent phytochemicals, which plays important role in antioxidant, anti-inflammatory, cytotoxicity assays. The further research is needed to identify the exact mechanism is by action of one or combination of active phytochemicals.

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Membrane stabilizing activity — a possible mechanism of action for the anti-inflammatory activity of Cedrus deodara wood oil

Cited by 412 publications

References 4 publications

In vitro Antioxidant and anti-inflammatory properties of selected Moroccan medicinal plants

In vitro Antioxidant and anti-inflammatory properties of selected Moroccan medicinal plants

Buffalo (Bubalus bubilis) colostrum and milk fat globule membrane fractions are potent antioxidants

Phytochemical analysis and in vitro antioxidant, antimicrobial, anti-inflammatory and cytotoxicity activities of wood rotting fungi, Trametes ochracea

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