Membrane Status and in Vitro Capacitation of Porcine Sperm Preserved in Long-Term Extender at 16°c

Conejo-Nava, J.; Fierro, Reyna; Gutiérrez, C. M.; Betancourt, Miguel

doi:10.1080/01485010390204931

Cited by 18 publications

(4 citation statements)

References 21 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…39,40 PIAR values are similar to those reported by other authors. 28,31,37,[41][42][43] With 12 mM FE, the proportion of capacitated sperm diminished significantly compared with controls, whereas the noncapacitated proportion did not change. This decrease corresponds to an increase in the sperm that developed SAR.…”

Section: Discussionmentioning

confidence: 88%

Effects of Atrazine and Fenoxaprop-Ethyl on Capacitation and the Acrosomal Reaction in Boar Sperm

et al. 2009

View full text Add to dashboard Cite

Atrazine is a herbicide of the chloro-s-triazine family. It inhibits photosynthesis in plants and is an endocrine disruptor, but its effects on human health are controversial. Fenoxaprop-ethyl, an aryloxy phenoxyalkanoic acid herbicide, inhibits the biosynthesis of fatty acids and provokes depolarization of membranes. The aim of this study is to evaluate the in vitro effects of both herbicides on capacitation, spontaneous acrosome reaction (SAR) and progesterone-induced acrosome reaction (PIAR) in boar sperm. Sperm capacitation is done in TALP-HEPES media for 4 hours. Capacitation and SAR are evaluated immediately; PIAR, 30 minutes later. LC50 for fenoxaprop-ethyl is 60 micromolar [corrected] and 40 micromolar [corrected] for atrazine. Fenoxaprop-ethyl induces capacitation at 60 micromolar [corrected] and SAR at all concentrations, also increases significantly PIAR. Atrazine decreased capacitation whereas increase significantly SAR and PIAR at all concentrations. It seems that fenoxaprop-ethyl and atrazine accelerate the capacitation and the acrosomal reaction, possibly via plasma membrane destabilization.

show abstract

Section: Discussionmentioning

confidence: 88%

Effects of Atrazine and Fenoxaprop-Ethyl on Capacitation and the Acrosomal Reaction in Boar Sperm

et al. 2009

View full text Add to dashboard Cite

show abstract

“…Spermatozoa with poor motility have a lower cholesterol content in the cell membrane in comparison with spermatozoa that exhibit higher motility (Am-in et al 2011). It has been demonstrated that semen cooling induces changes in the cell membrane of spermatozoa that affect their motility (Green and Watson 2001;Conejo-Nava et al 2003;Niżan´ski and Klimowicz 2005). Boar semen is particularly sensitive to thermal shock, thus its storage temperature plays an important role in artifical insemination (AI) protocols.…”

Section: Discussionmentioning

confidence: 96%

Assessment of changes in sperm cell membrane integrity occurring during the storage of semen from genetically different males using two diagnostic methods

Wysokińska

Kondracki

2014

Can. J. Anim. Sci.

View full text Add to dashboard Cite

Wysokin´ska, A. and Kondracki, S. 2014. Assessment of changes in sperm cell membrane integrity occurring during the storage of semen from genetically different males using two diagnostic methods. Can. J. Anim. Sci. 94: 601Á606. The present study was carried out to assess changes in sperm cell membrane integrity occurring during the storage of semen collected from genetically different domestic male pigs. The study was aimed at assessing differences in the course of changes in the integrity of cell membranes in spermatozoa produced by males with different degrees of genetic diversity (pure-bred males, two-breed hybrids and multi-breed crosses) and testing the usefulness of two methods of sperm cell membrane integrity evaluation, based on material collected from genetically different males. The experiments were conducted on 56 ejaculates collected from 28 domestic male pigs. The examination of sperm cell membrane integrity was performed three times for each ejaculate, i.e., after 1 h, after 24 h and after 48 h from collection. The preparations for analysing cell membrane integrity were made using two methods: the SYBR 14/PI method and the eosinÁnigrosin method. It was found that both SYBR 14/PI and eosinÁnigrosin staining methods make it possible to successfully assess the integrity of the plasma membrane of domestic pig sperm cells under in vitro conditions. Hybrid pig spermatozoa, especially those from multibreed crosses, better retain the integrity of their plasmalemmas than the spermatozoa of pure-bred boars. The ejaculates of Hypor cross-breed boars assessed after 1, 24 and 48 h of storage contain more spermatozoa with intact cell membranes than the ejaculates of pure-bred Duroc and Pietrain boars. The ejaculates of Hypor boars also show fewer decaying spermatozoa than those produced by pure-bred boars.

show abstract

“…Storage induces many age-related changes that disrupt metabolic processes, increase lipid peroxidation, and cause damage to sperm structures [ 14 , 28 , 36 , 37 ]. In the present study, the percentage of spermatozoa with normal morphology decreased over time, but the prevalence of morphological defects differed across storage variants.…”

Section: Discussionmentioning

confidence: 99%

Changes in the Morphology and Antioxidant Status of European Red Deer Sperm Stored in the Epididymides and in a Liquid State

Neuman,

Orzołek,

Steiner-Bogdaszewska

et al. 2024

Animals

View full text Add to dashboard Cite

The aim of this study was to evaluate the motility, morphology, and antioxidant status of European red deer sperm stored in a liquid state (variant I) and in the epididymides (variant II). Spermatozoa were harvested post-mortem from the cauda epididymis. Sperm samples in both variants were stored for up to six days (D6) at 5 °C. Spermatozoa were assessed for motility, viability, morphology, activity of antioxidant enzymes (superoxide dismutase, SOD; glutathione peroxidase, GPx; catalase, CAT), and lipid peroxidation (malondialdehyde, MDA, content). Sperm samples were analyzed on storage days 0, 2, 4, and 6 (D0-D6). Storage time and storage method significantly (p ≤ 0.05) influenced the examined variables. On D2, a decrease in motility and acrosomal integrity was observed in both storage variants, whereas a decrease in viability and an increase in MDA content were noted in spermatozoa stored in the epididymides. On D4, higher values of SOD and GPx activity and MDA content were noted in variant I than in variant II. Catalase activity was very low. GPx is the key enzyme that participates in the reduction of hydrogen peroxide in sperm cells. Spermatozoa stored in a liquid state were characterized by higher motility and viability, improved morphology and antioxidant status than those stored in the epididymides; therefore, liquid storage is more recommended for short-term preservation of epididymal spermatozoa.

show abstract

Membrane Status and in Vitro Capacitation of Porcine Sperm Preserved in Long-Term Extender at 16°c

Cited by 18 publications

References 21 publications

Effects of Atrazine and Fenoxaprop-Ethyl on Capacitation and the Acrosomal Reaction in Boar Sperm

Effects of Atrazine and Fenoxaprop-Ethyl on Capacitation and the Acrosomal Reaction in Boar Sperm

Assessment of changes in sperm cell membrane integrity occurring during the storage of semen from genetically different males using two diagnostic methods

Changes in the Morphology and Antioxidant Status of European Red Deer Sperm Stored in the Epididymides and in a Liquid State

Contact Info

Product

Resources

About