Memristive Biosensors Integration With Microfluidic Platform

Vallero, Alessandro; Tzouvadaki, Ioulia; Puppo, Francesca; Doucey, Marie‐Agnès; Delaloye, Jean-François; Micheli, Giovanni De; Carrara, Sandro

doi:10.1109/tcsi.2016.2608959

Cited by 14 publications

(7 citation statements)

References 21 publications

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“…Although nanowire-based detection of protein cancer markers has clearly evolved from the measurement of purified markers spiked into buffer to the measurement of markers spiked into healthy human serum, a very limited number of studies have quantified these markers in cancer patient blood-derived specimens [27,28]. Along the same lines, to date only two studies have reported the detection of a spiked antigen in cancer patient tissues using antibody-based FET [29,30] or memristor sensors [31]. In these Box 2.…”

Section: Protein Cancer Markersmentioning

confidence: 99%

“…studies, a spiked rabbit antigen was detected in the fM range in the presence of a 100 000-fold molar excess of non-specific human proteins [29][30][31]. The main limitations of nanowire sensors in detecting cancer markers in clinical specimens are the presence of salts that decrease the Debye length, the need for individual sensor calibration, and the multiplex capabilities of nanowire sensors which are so far limited to a maximum of three analytes.…”

Section: Protein Cancer Markersmentioning

confidence: 99%

“…The main limitations of nanowire sensors in detecting cancer markers in clinical specimens are the presence of salts that decrease the Debye length, the need for individual sensor calibration, and the multiplex capabilities of nanowire sensors which are so far limited to a maximum of three analytes. Recently, an alternative detection scheme using FET [29,32] and memristor [7,[10][11][12]31] sensors in ambient air significantly increased Debye length. Furthermore, an alternative method for internal calibration associated to a complementary metal-oxide semiconductor (CMOS)-compatible fabrication process yielded reproducible detections traceable to a calibration standard [33].…”

Section: Protein Cancer Markersmentioning

confidence: 99%

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Nanowire Sensors in Cancer

Doucey

Carrara

2019

Trends in Biotechnology

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In 2006, the group of Dr C.M. Lieber pioneered the field of nanowire sensors by fabricating devices for the ultra-sensitive label-free detection of biological macromolecules. Since then, nanowire sensors have demonstrated their ability to detect cancer-associated analytes in peripheral blood, tumor tissue, and the exhaled breath of cancer patients. These innovative developments have marked a new era with unprecedented detection performance, capable of addressing crucial needs such as cancer diagnosis and monitoring disease progression and patient response to therapy. The ability of nanowire sensors to identify molecular features of patient tumor represents a first step toward precision medicine, and their integration into portable devices has the potential to revolutionize cancer diagnosis and patient monitoring.

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Section: Protein Cancer Markersmentioning

confidence: 99%

Section: Protein Cancer Markersmentioning

confidence: 99%

Section: Protein Cancer Markersmentioning

confidence: 99%

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Nanowire Sensors in Cancer

Doucey

Carrara

2019

Trends in Biotechnology

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“…As a more extended version of this scheme, multiple biomarkers can be detected paving the way for multi-panel applications and simultaneous bio-detection of different biomarkers on the same chip. Having proven the multi-panel memristive bio-sensing paradigm, this scheme can be combined in a straight-forward way with the memristive biosensing board prototype [10] as well as with the microfluidic circuits especially designed for memristive biosensors [15], as illustrated in the complete scheme shown in Fig. 5.…”

Section: ) Label-free Sensingmentioning

confidence: 99%

Multi-Panel, On-Single-Chip Memristive Biosensing

Tzouvadaki¹,

Tuoheti

Lorrain

et al. 2019

IEEE Sensors J.

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Memristive biosensors have demonstrated excellent capabilities for ultrasensitive bio-detection. In the present work, memristive biosensing chips are designed, fabricated and implemented in a for the first time presented multi-panel on-chip detection for discrete sensing of a target molecule through separate functionalization of individual devices on the same chip. The biosensing scheme is validated by means of labeled (i.e. fluorescence) and label-free (i.e. electrical) characterization methods. This novel memristive multi-panel sensing paradigm paves the way for fast and ultrasensitive PoC (point-of-care) devices allowing the detection of specific targets in complex matrixes where non-specific molecules are present as well, and opening great potential for the application of memristive phenomena in multiplexed ultrasensitive bio-detection and theranostics.

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“…Typically poly-dimethyl-siloxane (PDMS) [11] is etched and bonded with the help of oxygen plasma [12] or di-rectly molded [13] on to Si circuit chips, in order to create microfluidic channels. Although these methods are fast and cost effective, the yield and the chemical stability against solvents which can be used in the microfluidic channels are the main drawbacks limiting the applications.…”

Section: Introductionmentioning

confidence: 99%

BiCMOS Integrated Microfluidic Packaging by Wafer Bonding for Lab-on-Chip Applications

Inac

2017

2017 IEEE 67th Electronic Components and Technology Conference (ECTC)

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In this paper, a fully BiCMOS integrated microfluidic technology platform for Lab-on Chip (LoC) applications is presented. Fusion bonding and adhesive bonding techniques are applied to realize a 3-wafer-stack integration. A glass wafer is used on top of the BiCMOS wafer and the Si channel wafer to enable simultaneous optical and electrical measurements. An alignment accuracy of less than 1 µm between BiCMOS and channel wafer is achieved while having channel heights between 30 and 100 µm. All the microfluidic process integration steps are kept below 300 • C. The microfluidic inlet and the outlet are positioned on the backside of the wafer stack to provide both the optical inspection and the electrical measurements from the wafer's front side. The developed wafer level integration technique provides high throughput solutions for emerging chemical and biological LoC applications.

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Memristive Biosensors Integration With Microfluidic Platform

Cited by 14 publications

References 21 publications

Nanowire Sensors in Cancer

Nanowire Sensors in Cancer

Multi-Panel, On-Single-Chip Memristive Biosensing

BiCMOS Integrated Microfluidic Packaging by Wafer Bonding for Lab-on-Chip Applications

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