MEMS-based confocal laser scanning microscope for in vivo imaging

Abstract

With a cardanically mounted micromirror a confocal laser scanning microscope for in vivo imaging was built. A resolution of 0.6 µm laterally and 10 µm axially allows to image tissue and cells in good quality. Samples of skin and adhered cells are imaged either in reflection or in fluorescence with an excitation wavelength of 682 nm. Fluorescence of Indocyanine Green is detected in the wavelength range above 730 nm.