Mercury-Mediated Epitaxial Accumulation of Au Atoms for Stained Hydrogel-Improved <i>On-Site</i> Mercury Monitoring

“…Nevertheless, fluorescence imaging and Coomassie brilliant blue dye staining cannot recognize proteins when protein concentrations are lower than 0.1 mg mL –1 (Figure S3), suggesting their low sensitivities. We were remarkably inspired by our previous researches about the incubation of active hydrogels in HAuCl 4 solutions that the free diffusion of HAuCl 4 from the solutions to the hydrogels enabled the in situ formation of hydrogel network-stabilized plasmonic Au nanoparticles. , After protein electrophoresis, MoS 2 /AG hydrogels were immediately immersed into the HAuCl 4 solutions. The color of the MoS 2 /AG hydrogel turned from green to pink after 20 min (Figure e).…”

Section: Resultsmentioning

confidence: 99%

Electrophoretic Microplate Protein Identification Based on Gold Staining of Molybdenum Disulfide Hydrogels

Zhang,

Luo,

Wang

et al. 2024

Anal. Chem.

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Numerous high-performance nanotechnologies have been developed, but their practical applications are largely restricted by the nanomaterials' low stabilities and high operation complexity in aqueous substrates. Herein, we develop a simple and high-reliability hydrogel-based nanotechnology based on the in situ formation of Au nanoparticles in molybdenum disulfide (MoS 2 )doped agarose (MoS 2 /AG) hydrogels for electrophoresis-integrated microplate protein recognition. After the incubation of MoS 2 /AG hydrogels in HAuCl 4 solutions, MoS 2 nanosheets spontaneously reduce Au ions, and the hydrogels are remarkably stained with the color of as-synthetic plasmonic Au hybrid nanomaterials (Au staining). Proteins can precisely mediate the morphologies and optical properties of Au/MoS 2 heterostructures in the hydrogels. Consequently, Au staining-based protein recognition is exhibited, and hydrogels ensure the comparable stabilities and sensitivities of protein analysis. In comparison to the fluorescence imaging and dye staining, enhanced sensitivity and recognition performances of proteins are implemented by Au staining. In Au staining, exfoliated MoS 2 semiconductors directly guide the oriented growth of plasmonic Au nanostructures in the presence of formaldehyde, showing environment-friendly features. The Au-stained hydrogels merge the synthesis and recognition applications of plasmonic Au nanomaterials. Significantly, the one-step incubation of the electrophoretic hydrogels leads to high simplicity of operation, largely challenging those multiple-step Ag staining routes which were performed with high complexity and formaldehyde toxicity. Due to its toxic-free, simple, and sensitive merits, the Au staining integrated with electrophoresis-based separation and microplate-based high-throughput measurements exhibits highly promising and improved practicality of those developing nanotechnologies and largely facilitates in-depth understanding of biological information.

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“…Their capability to discriminate between various analytes within complex sample matrices ensures the precision of the measurements. Moreover, their compatibility with miniaturization and automation contributes to their versatility, allowing seamless integration into portable devices. , In addition to their characteristic high sensitivity, fluorescence-based sensing methods offer the advantage of rapid, real-time data acquisition, enabling continuous monitoring across a broad spectrum of industrial and healthcare applications. Consequently, these methods have garnered significant attention among researchers.…”

Section: Introductionmentioning

confidence: 99%

Triazine-Adenine Anchored Porous Organic Polymer: An Integrated Approach as a Fluorescent Sensor and Molecular Sieve for Mercury Ions

Vijayan,

Ramakrishnan,

Elambalassery

2024

ACS Appl. Polym. Mater.

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The current scenario of environmental pollution attributed to industrial ecotoxicants envisages the imperative necessity not just to detect highly toxic heavy metal ions but also to concurrently eliminate them, to ensure the sustainable safeguard of the environment. However, the contemporaneous detection and removal of mercury ions is seldom reported. Herein, a triazinebased porous organic polymer, POP CCAD, consisting of nitrogen-rich binding sites, has been designed and synthesized via a simple nucleophilic substitution reaction using cyanuric chloride and adenine as the starting materials. POP CCAD could achieve its application as a bifunctional material for the fluorescence sensing of Hg 2+ with a detection limit of 8.4 nM and a removal capacity of 460 mg g −1 . This work reveals the capability of porous organic polymers, serving not only as fluorescent sensors for mercury ions but also as molecular sieves for their separation.

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Mercury-Mediated Epitaxial Accumulation of Au Atoms for Stained Hydrogel-Improved On-Site Mercury Monitoring

Cited by 11 publications

References 41 publications

Paper-based colorimetric hyperammonemia sensing by controlled oxidation of plasmonic silver nanoparticles

Paper-based colorimetric hyperammonemia sensing by controlled oxidation of plasmonic silver nanoparticles

Electrophoretic Microplate Protein Identification Based on Gold Staining of Molybdenum Disulfide Hydrogels

Triazine-Adenine Anchored Porous Organic Polymer: An Integrated Approach as a Fluorescent Sensor and Molecular Sieve for Mercury Ions

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