Mesenchymal stem cell‐conditioned media: A novel alternative of stem cell therapy for quality wound healing

Abstract

Mesenchymal stem cells‐conditioned media (MSCs‐CM) contains several growth factors and cytokines, thus may be used as a better alternative to stem cell therapy, which needs to be elucidated. The present study was conducted to evaluate the therapeutic potential of caprine, canine, and guinea pig bone marrow‐derived MSCs‐CM in excision wound healing in a guinea pig model. MSCs were obtained from bone marrow, expanded ex vivo and characterized as per ISCT criteria. CM was collected assayed by western blot to ascertain the presence of important secretory biomolecules. Quantitative estimation by enzyme‐linked immunosorbent assay was done for a vascular epidermal growth factor (VEGF) and interleukin‐6 (IL‐6) in caprine MSCs‐CM and optimum time for collection of CM was decided as 72 hr. CM from all the species was lyophilized by freeze‐drying method. Full‐thickness (2 × 2 cm2) excision skin wounds were created in guinea pigs (six animals in each group) and respective lyophilized CM mixed with laminin gel was applied topically at weekly interval. On Day 28, histopathological examinations of healed skin were done by hemotoxylin and eosin staining. MSCs were found to secrete important growth factors and cytokines (i.e., VEGF, transforming growth factor‐β1, fibroblast growth factor‐2, insulin‐like growth factor‐1, stem cell factor, and IL‐6) as demonstrated by immunohistochemistry and western blot assay. It was found that allogenic and xenogenic application of CM significantly improved quality wound healing with minimal scar formation. Thus, MSCs‐CM can be used allogenically as well as xenogenically for quality wound healing.

The Potential of a Hair Follicle Mesenchymal Stem Cell-Conditioned Medium for Wound Healing and Hair Follicle Regeneration

“…This feature has been indicated in the previous research that found that CM obtained from BM-MSC significantly accelerated scratch wound closure in L929 fibroblasts and HaCaT keratinocytes, supporting the application of MSC-CM as a therapeutic agent in cutaneous wound healing [26]. Accordingly, the latest study by Joseph et al also found that MSC-CM can be used to improve the quality of wound healing with minimal scar formation [2]. Using CM from MSC culture increases fibroblast and basal cell proliferation, collagen synthesis, and improve vascularization in the process of skin wound healing [9,11].…”

“…Skin injuries are the most commonly found injury caused by blisters, burns, cuts, and ulcers of various severities [1]. If left untreated, any skin injury can interfere with its protective function and cause body tissues to be easily exposed to mechanical damages and microbial infections [2]. Other problems that may accompany wound healing are excessive healing, delayed wound healing, or lack of skin appendages [1].…”

“…Recently, the increasing demand for quality improvement of damaged skin has led to the shifting focus of wound repair to the restoration of anatomy and function of the skin [3]. Thus, identifying an effective approach to enhance the wound healing process is indispensable [1][2][3][4].…”

“…Traditionally, the main purpose of wound care is to obtain wound closure at the earliest time [1]. Nowadays, the use of stem cell-based regenerative therapy to renew physiological tissue and promote the regeneration of various types of wounds is increasingly popular [2,5]. Mesenchymal stem cells (MSCs) have been reported to have therapeutic potential after skin injury and extensive proliferation and differentiation capacity that have the ability to suppress the stimulation of immune cells and participate in tissue repair and regeneration [5][6][7][8].…”

Mesenchymal Stem Cells and Their Derived Conditioned Media as Crucial Cell Candidates for Wound Healing: A Letter

“…research study performed by Joseph et al reported that even allogenic and xenogenic use of MSC-derived conditioned media from caprine, canine, and guinea pig bone marrow could be a suitable option for healing wound defects with decreased scar production(1). The study by Joseph demonstrated that MSC-derived conditioned media may be considered an alternative for stem cell-based therapy to facilitate quality wound healing(1).…”